4葡聚糖硫酸酯的制备及其抗凝血活性

β-1,4-葡聚糖硫酸酯的制备及其抗凝血活性王兆梅，李琳，郭祀远，肖凯军(510640)摘要：对微晶纤维素（MCC）进行硫酸酯化修饰，得到多种β-1,4-葡聚糖硫酸酯（GS），其硫酸取代度（Ds）在1.10～1.70范围内。选择Ds为1.70的GS用于结构分析和抗凝血活性研究。IR分析表明，MCC通过硫酸化反应引入了硫酸酯基13CNMR揭示，硫酸酯化主要发生在C6，部分在C2，而C3位不发生硫酸酯化反应。凝血分析表明，0.2mg/L的GS即可显著延长血浆的活化凝血活酶时间(tAPTT)和凝血酶时间(tTT)，使血浆tAPTT延长两倍，所需GS的剂量为0.7mg/L，低于活性为150IU/mg的肝素，在一定质量浓度范围内，GS的体外抗凝血活性与肝素相当。显色分析揭示，GS的抗凝血机制主要在于通过抗凝血酶AT-Ⅲ的调节作用抑制凝血因子Ⅱa和Xa的活性。关键词：葡聚糖硫酸酯；抗凝血；机制中图分类号：TQ35;O614文献标识码：A文章编号：0253-2417(2006)04-0001-05PreparationandAnticoagulationActivityofβ­1,4­GlucanSulfateWANGZhao­mei,LILin,GUOSi­yuan,XIAOKai­jun(ResearchInstituteofLightIndustryandChemicalEngineering,SouthChinaUniversityofTechnolage,Guangzhou510640,China)Abstract:Variousβ­1,4­glucansulfates(GS)weresynthesizedbysulfateofmicrocrystallinecellulose.Theirsulfatesubstitutedegree(Ds)variedintherangeof1.10to1.70.GSwithDsof1.70wasselectedforstructuralanalysisandanticoagulationinvestigation.IRspectrashowedtheintroductionofsulfategroupsbysulfation.13CNMRindicatedthatsulfationoccurredmainlyatC6,partiallyatC2andnosulfatesubstitutionoccurredatC3.Coagulationassaysshowedthat0.2mg/LGScouldsignificantlyprolongtAPTTandtTTinplasma.ThedosageofGSrequiredtodoubletAPTTofnormalhumanplasmawas0.7mg/L,lowerthanthatofheparinwiththeanticoagulationactivityof150IU/mg.Itspotentofanticoagulationinvitroreachedtheefficacyofheparinindefiniterangeofconcentration.ChromogenicanalysisrevealedthattheanticoagulationmechanismofGSwasmainlyduetotheinhibitionofthecoagulationfactorsⅡa&XaactivitiesmediatedbyantithrombinAT­Ⅲ.Keywords:glucansulfate;anticoagulation;mechanismE1级胶合板用脲醛树脂的13CNMR定量分析金立维，王春鹏，周道兵，储富祥*，赵临五林产化学工程重点开放性实验210042）摘要：采用13CNMR定量分析了由选定碱-酸-碱工艺所合成的E1级胶合板用脲醛树脂。结果表明：在初始碱性阶段有70.27%的甲醛分子转化成了羟甲基脲，24.83%的甲醛分子转化成了亚甲基醚键。在随后的酸性缩聚阶段中，64%的羟甲基脲发生断裂，放出甲醛，缩聚成亚甲基键及亚甲基醚键。在酸性阶段加入的第二批尿素与树脂中的游离甲醛形成羟甲基脲。超过一半的亚甲基醚键在最后的碱性保温阶段发生分解，并与此时加入的第三批尿素生成羟甲基脲。关键词：脲醛树脂；羟甲基脲；亚甲基醚；胶合板中图分类号：TQ323；TQ433文献标识码：A文章编号：0253-2417(2006)04-0006-05Quantitative13CNMRAnalysisofUrea-formaldehydeResinforE1-typePlywoodJINLi­wei,WANGChun­peng,ZHOUDao­bing,CHUFu­xiang,ZHAOLin­wu(InstituteofChemicalIndustryofForestProducts,CAF;KeyandOpenLab.onForestChemicalEngineering,SFA,Nanjing210042,China)Abstract:Theurea­formaldehyde(UF)resinsforE1­typeplywoodsynthesizedbyspecificprocedurewerequantitativelyexaminedbythe13CNMRspectroscopy.Itwasprovedthat70.27%offormaldehydemoleculesweretransformedintohydroxymethylureaand24.83%formaldehydemoleculesweretransformedintomethylene­etherbondduringtheinitialalkalinereactionstage（pHvalue7.2-7.8）.About64%ofhydroxymethylgroupsformedintheinitialalkalinereactionstagewerepolymerizedduringthesubsequentacidicreactionstage（pHvalue5.4-5.6）byformingmethyleneormethylene­etherbondsandreleasingsomeformaldehyde.Thefreeformaldehydeofresincouldreactwiththeseconddosageofureaduringthenextacidicreactionstage（pHvalue6.5-6.9）toformhydroxymethylureas.Morethanhalfofmethylene­etherbondsweredecomposedduringthelastalkalinecoolingstage（pHvalue7.5-8.0）andreactedwiththethirddosageofureatoformhydroxymethylurea.Keywords:urea­formaldehyderesin;hydroxymethylurea;methylene­ether;plywoodAH型非贵金属催化松香歧化反应动力学研究王亚明，王路辉，蒋丽红，王震(650224)摘要：用AH型非贵金属催化剂催化松香歧化反应，考察了搅拌速率、催化剂粒度、反应温度、反应时间对反应的影响，建立了动力学方程。确认在240～270℃范围反应为一级,求出在240、250、260和270℃时的反应速率常数分别为2.9×10-3、3.1×10-3、1.42×10-2和2.51×10-2min-1。表观活化能E为184.80×103kJ/mol，频率因子A=1.453×1016。关键词：AH型非贵金属催化剂；松香歧化反应；动力学方程中图分类号：TQ351.47；TQ426文献标识码：A文章编号：0253-2417(2006)04-0011-03ResearchonReactionKineticsofDisproportionationofGumRosinCatalyzedbyAHNon­nobleMetalCatalystWANGYa­ming,WANGLu­hui,JIANGLi­hong,WANGZhen(InstituteofBiologicalandChemicalEngineering,KunmingUniversityofScienceandTechnology,Kunming650224,China)Abstract:ThereactionofdisproportionationofgumrosincatalyzedbyAHnonnoblemetalcatalystwasstudied,andtheeffectsofreactiontemperature,reactiontime,particlesizeofcatalyst,andstirspeedwereinvestigated.Itwasfoundthatthereactionwasofthefirstorderat240-270℃.Reactionrateconstantswere2.9×10-3,3.1×10-3,1.42×10-2,2.51×10-2min-1at240,250,260and270℃,respectively.ApparentactivationenergyEwas184.80×103kJ/mol,frequencyfactorAwas1.453×1016.Keywords:AHnon­noblemetalcatalyst；disproportionationofgumrosin；kineticequationFolin­Ciocalteu比色法测定橄榄中多酚含量的研究何志勇，夏文水*214036）摘要：采用Folin­Ciocalteu比色法研究了橄榄多酚的定量测定方法。结果表明：橄榄多酚提取液在加入Folin­Ciocalteu试剂1.5mL、10%Na2CO36mL、反应时间2h、温度30℃的条件下进行显色，并于765nm处测定其吸光值，多酚质量浓度在7～70mg/L范围内与吸光值有良好的线性关系，根据此线性回归方程进行橄榄多酚的定量测定，该方法简单方便、稳定性好、准确度高。关键词：橄榄；多酚；比色法中图分类号：TQ351.014文献标识码：A文章编号：0253-2417(2006)04-0015-04StudyonDeterminationofPolyphenolContentinCanariumalbum(Lour.)Rauesch.byFolin­CiocalteuColorimetryHEZhi­yong,XIAWen­shui(SchoolofFoodScienceandTechnology,SouthernYangtzeUniversity,Wuxi214036,China)Abstract:AquantitativedeterminationmethodofpolyphenolfromCanariumalbum(Lour.)Rauesch.wasstudiedbyFolin­Ciocalteucolorimetry.TheresultsshowedthatpolyphenolcontentofC.albumcouldbewellcalculatedaccordingtotheircolorime­tricabsorptionat765nmbyapplyingFolin­Ciocalteureagent1.5mLand10%Na2CO36mL,2hat30℃forcolorimetricreaction,andthelinearrangeofstandardcurvewas7-70mg/L.Themethodestablishedwasveryc