杉木人工林不同发育阶段根际与非根际土壤微生物变化趋势

:1997209203:(100091):,,,;,,,,,;,,,,,,;,,,,:,,,THECHANGESOFTHESOILMICROORGANISMINRHIZOSPHEREANDOUTSIDEINDIFFERENTDEVELOPINGSTAGESOFTHECHINESEFIRPLANTATIONJiaoRuzhenYangChengdong(TheResearchInstituteofForestry,CAFBeijing100091)Abstract:TheisolationsofsoilmicroorganismindifferentdevelopingstagesoftheChinesefirplantationwereinvestigated.Itwasfoundthattheamountsofrhizospherebacteriaandthebacteriainjuvenileandmaturestandswerehigherthanthoseofnon2rhizosphereandthebacteriainmediumstands.Theamountsofactinomyceteshavenosignificantchangeamongrhizosphereandnon2rhizospheresoils.TheisolationsweremoreabundantinjuvenileandmaturestandsthanthatinmediumstandsinwhichonlyAlbosporus,Flavus,Griseofuscuswerefound.Theamountsoffungisignificantlydeclinedfromjuveniletomediumstandsandincreasedtomaturestands.ThepercentageofPenicilliuminmediumrhizospheresoilishigherthanthoseinthejuvenileandmaturestands.Theresultssuggestedthatthecomparisonoftherhizosphereandnon2rhizospheresoilsisusefultoshowtherelationshipbetweenthechangeofsoilcharactersandtheChinesefirgrowth,controllingsoildegradationandimprovingproductionoftheplantation.Keywords:Chinesefirplantation,Developingstage,Microorganismofrhizosphere,Soilmicroorganism,17,,,,,,60,,,,,,,,,,,,35119991SCIENTIASILVAESINICAEVol135,No11Jan.,1999,,1,,2730N,11430E,,,,,,,1715,159317mm,50%46,268d,,,,,,,,22.119965(16)()3,(,1985)[1],,040cm2.2(,1985)[1]:,10-3;,10-2;,10-2,3,2528,1836h35d710d33,,(RE,NE,1984)[2]33.11,,,;,;,,,1,,,7%NaCl50pH5.732(,1978)[3],()2,23.33,,,;,;,,,,,,45351Tab.1Thenumberofrhizospheresoilmicrobeandnon2rhizospheresoilmicrobeindifferentdevelopingstagesoftheChinesefirplantationPlaceofselectedsamplesDevelopingstagesAgeofstandSpotofselectedsamplesMoisturecoefficient(%)1Amoutofbacteriain1gramsoil(Individual/g)1Amoutoffungiin1gramsoil(Individual/g)1Amoutofactinomycetein1gramsoil(Individual/g)NianzhuforestfarmJuvenile4RhizosphereNon2rhizosphere77117418171991061317810641210431910401261040140104NianzhuforestfarmMedium16RhizosphereNon2rhizosphere6912711141781062142106212104214104116104814104NianzhuforestfarmMature26RhizosphereNon2rhizosphere7013711751161062161106317104219104710104711104ShangcunforestfarmJuvenile7RhizosphereNon2rhizosphere7512801681821062188106212104119104418104416104ShangcunforestfarmMedium18RhizosphereNon2rhizosphere7313761431751062151106118104116104715104615104ShangcunforestfarmMature25RhizosphereNon2rhizosphere80147911416310631841063101047161042161044161043.22Tab.2ThemorphologicalandbiochemicalcharacteristicsofcultureandcellofsoilbacteriaindifferentdevelopingstagesNum2berThemorphologicalcharacteristicsofcultureandcellHy2drolyzestarchV2PAcetyl2methyl2carbinoltestM2RMethylredtestGlucoseoxidizeGlucoseleavenGelatinliquefyCatalaseNameofbacteria9501,,Flatandcrude,rods,gemmaincentre+++++++(Bacillussub2tilis)9502(),,Largeandsmooth,rods,gemmaincentre+---+++(Bacillusmegaterium)9503,,G2smallandfluoresceblue,rods,Gram2nega2tive-+--+++(AgrobacteriumConn)9504,G+,Yellow,Gram2positive,rodsinirregularform+--++++(CellulomonasBergey)551:29505,G+,,Rhizamorphoid,Gram2positiverods,havinggem2ma++++++-(Bacillusmy2coides)9506,G+,Orange2coloured,Gram2positive,coccoidform+--++-+(Mi2crococcusCohn)9507,,G2violet,coccobacillus,Gram2negative---++-+(Chromobac2terium)9508,,G+Yellow,coccoid,Gram2positive----+++(Staphyloco2ccus)9509,Brown,bigcellwiththicksporo2derm-+--+++(Azo2tobacterBeijer2inck)G+,G2;V2P,M2R.G+isGram-positive,G-isGramnegative;V2PisatestaboutAcetyl-mathyl-carbinol,M2RisatestaboutMethylredtest3.3Tab.3Thepercentageofallsortsbacteriainrhizosphereandnon2rhizospheresoils.PlaceofselectedsamplesDevel2opingstagesSpotofselectedsamplesNianzhuforestfarmJuvenileRhizosphereNon2rhizosphere151316101112131970107510214018017114015014214NianzhuforestfarmMediumRhizosphereNon2rhizosphere13139191415191566126816112217112013018118NianzhuforestfarmMatureRhizosphereNon2rhizosphere13197172214411052174119311314410015413314117ShangcunforestfarmJuvenileRhizosphereNon2rhizosphere181621111916511954171910115216410017013213317ShangcunforestfarmmatureRhizosphereNon2rhizosphere141412102419181856136112014410512218ShagcunforestfarmMediumRhizosphereNon2rhizosphere201326102715371442133117615119015011015214219,,,,6535,,,,,,,(De2mass,1967),,,3.4,(Penicillium)(Mucor)(Trichoderma)(Aspergillus),scytalidiumlignicolaDichlaenaLentisci;Mon2tagueetDurieTrimmatostromasalicis,,4(,1973)[7].4Tab.4Thepercentageoffungiinrhizosphereandnon-rhizosoilspheresoilsPlaceofselectedsamplesDevelopingstagesAgeofstandSpotofselectedsamplesPercentageofvariouskindsoffungiPenicillumMucorAspergillusTrichodermaNianzhuforestfarmJuvenile4RhizosphereNon2rhizosphere6410421518181013201712151318NianzhuforestfarmMedium16RhizosphereNon2rhizosphere801050101010201020101210NianzhuforestfarmMature26RhizosphereNon2rhizosphere5619481519122132010319915ShangcuforestfarmJavenile7RhizosphereNon2rhizosphere701045121210181731013103101311ShangcunforestfarmMedium18RhizosphereNon2rhizosphere8017461215147117121815ShagcunforestfarmMature25RhizosphereNon2rhizosphere58131111161744141111251033134,,