1211*1*1.2100962.210008Q331A1005-7021200504-0077-05ApplicationofCapillaryElectrophoresisinMicrobeSeparationandDetectionYANGYu-zhi12ZHANGChun-xiu1TANGZu-ming1LUZu-hong11.KeyLabofMoleculeandBiomoleculeunderMinist.ofEducat.Dept.ofBiomedicalEngin.SEUniv.Nanjing2100962.NanjingGulouHosp.Nanjing210008AbstractCapillaryelectrophoresiscanbeusedtoisolateandanalyzeawidevarietyofinorganicandorganicsmallmoleculesbio-macro-moleculessuchasproteinsnucleicacidsandmicrobesbacteriaviruses.Basicprincipleearlyexplorationtheexistingproblemsandthenewestprogressofitinmicrobeseparationanddetectionwereem-phasized.Keywordscapillaryelectrophoresismicrobebacteriavirus1980CapillaryElec-trophoresisCE1CZECGECITPCIEFACE12004-11-16602230028632002AA2Z20412004AA30207*7720057254JOURNALOFMICROBIOLOGYJuly2005Vol.25No.4191987HjertenTobaccomosaicvirusTMVLactobacilliuscasei2G-1~23G-G+15~50G+PI2~3G-PI4~5pH≥5G-G+3pH4Schnabel5μZetaξEηrμEξE1.5ηfkrfkr1~1.5rpH21.5nm10μmpHpHpHpH631987Hjerten2TMVL.caseipH8.5100μm260nmTMV220nmL.casei4min872519897CE14min26.6min28.3min32.8minCE1993EbersoleMcCormick8EnterococcusfaecalisStreptococcuspyogenesStreptococ-cusagalactiaeStreptococcuspneu-moniaeStaphylococcusau-reus90%100μm250cm211997PfetschWelsch3PseudomonasputidaPseudomonasspeciesAlcaligeneseutrophus9EbersoleMcCormick250μm250cmpHpH9.62~10mmolL20~30m2vs-141999ArmstrongcapillaryzoneelectrophoresisCZEp0.025%100μm27cm10minM.luteusE.aerogenesP.fluorescensS.cerevisiaeArmstrongpH6Shiniani0.01%S.enteritidisS.typhimurium10Girod11CCD974pHJ.Zheng12CCD0≠90≠51987HjertenTMVTMV2GrossmanSoaneTMV13KenndlerhumanrhinovirusHRV1996HRV14HRV153.6minHRVHRVRNA3.6min4.8minRNARNA3.6min4.8minRNA3.6minHRVHRV3.6min3.6minHRVOkunACEHRVmAb8F516HRVmAb8F5mAb8F5mAb8F5HRV14mAb17-IAHRVmAb3B10HRV2HRV2CEmAb3B10mAbHRV2Mann1757cm25mmolL9~10minpH7.025mmolL66.1PCRELISAPCRELISA2h3d20038Byoung-geounMoonYongseongKimCE182100μm40cm214nm30min08256.2E.coliS.saprophyticus2Armstrong219pH9.010000071.M.1996.2HjertenSElenbringKKilarF.etal.Carrier-freezoneelectrophoresisdisplacementelectrophoresisandisoelectricfo-cusinginahigh-performanceelectrophoresisapparatusJ.Chromatogr198740347-614300-4303.3.2M.1999.4D.W.ArmstrongL.He.RapiddeterminationofcellviabilityinsingleormixedsamplesusingcapillaryelectrophoresisLIFmicrofluidicsystemsJ.AnalChem2001734551-4557.5U.SchnabelC.H.FischerE.Kenndler.CharacterizationofcolloidalgoldnanoparticlesaccordingtosizebycapillaryzoneelectrophoresisJ.J.Microcol.Separ19979529-534.6D.W.ArmstrongG.SchulteJ.M.Schneiderheinzeetal.Separatingmicrobesinthemannerofmolecules.1.CapillaryelectrokineticapproachesJ.AnalChem1999715465-5469.7A.ZhuY.Chen.High-voltagecapillaryzoneelectrophoresisofredbloodcellsJ.J.Chromatogr1989470251-260.8R.C.EbersoleR.M.McCormick.SeparationandisolationofviablebacteriabycapillaryzoneelectrophoresisJ.Biotechnol-ogy1993111278-1282.9A.PfetschT.Welsch..Determinationoftheelectrophoreticmobilityofbacteriaandtheirseparationbycapillaryzoneelec-trophoresisJ.FreseniusJ.AnalChem1997359198-201.10T.ShinitaniK.YamadaM.Torimura.OptimizationofarapidandsensitiveidentificationsystemforSalmonellaenteritidisbycapillaryelectrophoresiswithlaser-inducedfluorescenceJ.FEMSMirobio.Lett2002210245.11M.GirodD.W.Armstrong.Monitoringthemigrationbehavioroflivingmicroorganismsincapillaryelectrophoresisusinglaser-inducedfluorescencedetectionwithacharge-coupleddeviceimagingsystemJ.Electrophoresis2002232048.12J.ZhengE.S.Yeung.MechanismofMicrobialAggregationduringCapillaryElectrophoresisJ.Anal.Chem200375818.13P.D.GrossmanD.S.Soane.Orientationeffectsontheelec-trophoreticmobilityofrod-shapedmoleculesinfreesolutionJ.Anal.Chem.1990621592.14U.ScnabelF.GroissD.Blaasetal.DeterminationofthepIofHumanRhinovirusSerotype2byCapillaryIsoelectricFocusingJ.AnalChem1996684300.15V.OkunB.RonacherD.Blaas.etal.Analysisofcommoncoldvirushumanrhinovirusserotype2bycapillaryzoneelectrophoresistheproblemofpeakidentificationJ.AnalChem1999712028.16V.OkunB.RonacherD.Blaas.etal.Affinitycapillaryelec-trophoresisfortheassessmentofcomplexformationbetweenvirusesandmonoclonalantibodiesJ.AnalChem2000724634.17B.MannJ.A.TrainaC.Soderblom.etal.CapillaryzoneelectrophoresisofarecombinantadenovirusJ.ChromatogrA2000895329.18Byoung-geounMoonYongseongKim.AnalysisofHealth-relat-edMicrobesbyCapillaryElectrophoresisJ.Bull.KoreanChem.Soc20032481203.19D.W.ArmstrongJ.M.Schneiderheinze.RapidIdentificationoftheBacterialPathogensResponsibleforUrinaryTractInfec-tionsUsingDirectInjectionCEJ.AnalChem2000724474.184