活性污泥中微生物种群特性及对底物降解的影响研究

***2004-11-302004-12-31***421101214036PCRERICDGGETGGEFISHT-RFLPPopulationcharacterizationofLicroorganisLinactivatedsludgeanditsinfluenceonsubstratedegradation.NIEYan-QiuDepartmentofArchitectureEngineeringHunanInstituteofTechnologyHengyang421101ChinaZHOUQingTheKeyLab.ofIndustrialBiotechnologyMinistryofEducationSouthernYangtzeUniversityWuxi214036ChinaCJEA200513425~28AbstractThechoiceofculturemediumofmicrobialisolationandmeasuringtechnologiesincludingPCRERICDGGETGGEFISHandT-RFLPareintroducedbriefly.Thenthemicrobialpopulationcharacterizationsintheactivatedsludgei.e.microbialdiversitymicrobialcommunitystructurestabilityanditsfunctionstabilityarestated.Finallyitispointedoutthatthemicrobialdiversityintheactivatedsludgeisthebaseofitscommunitystructurestabilitywhichisthepreconditionofitsfunctionstabilitybeingtheguaranteeofeffectivesubstratedegradation.Inreversethevarietyofsub-strateandenvironmentfactorwillalsoaffectthestabilityofcommunitystructureandfunctionandmaymakethepopula-tiondiversitychange.Thechangeofthemicrobialcommunitystructurestabilitydoesn'tkeepinstepwiththatofitsfunc-tionstability.KeywordsWastewatertreatmentPopulationcharacterMeasurementmethodReceivedNov.302004revisedDec.3120041OperationaltaxonomicunitOTUDiversityindexHPairwisesimilaritycoefficientCsHCs6HE-∑niNlnniN1CsE2ja+b*1002niNabDNAj21.1134Vol.13No.4200510ChineseJournalofEco-AgricultureOct.20051430.8%~42.9%230.2%0.1%0.2%pH7.0~7.527Poly-merasechainreactionPCREnterobacterialrepetitiveintergenicconsensusERIC126bp44bpDNAERIC-PCR3Denaturinggradientgelelec-trophoresisDGGETemperaturegradientgelelectrophoresisTGGEDNADNATGGEDGGE4FluorescenceinsituhybridizationFISHDNA5TerminalrestrictionfragmentlengthpolymorphismT-RFLPTerminalrestrictionfragmentanalysisTRFADNAT-RFLPPCRDNAT-RFLP151.22PCRrDNAAmplifiedrDNArestrictionanalysisARDRA144TGGE94TGGE3297%16MartinEschenhagena8FISHT-RFLPPP1Tetrasphaeraspp.PPMicrolunatusRhodocyclusMartinEschenhagena82P4TGGE216SrDNAV3-PCRTGGE100%TGGETGGE83.3%3AB2CODcrCODcrERIC-PCRStamperDavidM.9MembranebioreactorMBR0%~25%261390%ForneyL.J.10-35dDGGETRFA16SrDNAKlattChristianG.112DGGE7d50%CarolinaCasserly6UpflowanaerobicsludgebedUASBCH416SrRNA16SrDNAAnnetteF.Muttray12Abi-etaniphilaBleachedkraftmilleffluentBKME-9rRNArDNABKME-9BKME-9rRNArDNABKME-9rRNArDNApHBKME-9GavinCollins13VolatilefatacidVFACOD5kgm3d18࠷90d2KlattChristianG.112CODGhyootWouter142NP1NP2NPCODcrLuxmyB.S.15pHE61000~2000mL2pH57LuxmyB.S.161μm10μmpHCH4CH4BOD42731..2003432264~2702..2003433366~3773.ERIC-PCR.2003236705~7104.TGGE.200323101963~19695..2003.204~4036CarolinaCasserlyLeonardoErijman.MolecularmonitoringofmicrobialdiversityinanUASBreactor.InternationalBiodeterioration&Biodegradation2003527~127AllisonE.McCaigA.SusanJ.etal.Impactofcultivationoncharacterisationofspeciescompositionofsoilbacteriacommunities.FEMSMicrobiolEcol.20013537~488MartinEschenhagenaMarkusSchupplerbIsoldeRoskea.Molecularcharacterizationofthemicrobialcommunitystructureintwoactivatedsludgesystemsfortheadvancedtreatmentofdomesticeffluents.WaterResearch2003373224~32329StamperDavidM.WalchMarianneJacobsRachelN.Bacterialpopulationchangesinamembranebioreactorforgraywatertreatmentmon-itoredbydenaturinggradientgelelectrophoreticanalysisof16SrRNAgenefragments.AppliedandEnvironmentalMicrobiology2003692852~86010ForneyL.J.LiuJ.B.etal.StructureofmicrobialcommunitiesinactivatedsludgePotentialimplicationsforassessingthebiodegradabilityofchemicals.EcotoxicologyandEnvironmentalSafety20014940~5311KlattChristianG.LaParaTimothyM.Aerobicbiologicaltreatmentofsyntheticmunicipalwastewaterinmembrane-coupledbioreactors.BiotechnologyandBioengineering2003823313~32012AnnetteF.MuttrayZhontangYuWilliamW.Mohn.PopulationdynamicsandmetabolicactivityofPseudomonasabietaniphilaBKME-9withinpulpmillwastewatermicrobialcommunitiesassayedbycompetitivePCRandRT-PCR.FEMSMicrobiologyEcology2001382113GavinCollinsAdeleWoodsSharonMcHughetal.Microbialcommunitystructureandmethanogenicactivityduringstart-upofpsy-chrophilicanaerobicdigesterstreatingsyntheticindustrialwastewaters.FEMSMicrobiologyEcology200346159~17014GhyootWouterVerstraeteWilly.Reducedsludgeproductioninatwo-stagemembrane-assistedbioreactor.WaterResearch2000341205~21515LuxmyB.S.KuboT.YamamotoK.Sludgereductionpotentialofmetazoainmembranebioreactors.WaterScienceandTechnology20014410197~20216LuxmyB.S.NakajimaF.YamamotoK.Predatorgrazingeffectonbacterialsizedistributionandflocsizevariationinmembrane-separa-tionactivatedsludge.WaterScienceandTechnology2000423211~2172813