烟田杂草内生真菌的分离鉴定及生物学特性的初步研究

:2010-05-13;:2010-07-08:(2009009):(1980-),,,:*:,,,:E-mai:[e-mail]*张秀伟,桑维钧*,谢鑫,王莉爽,桂泽文(,550025):从烟田杂草中分离筛选出对烟草病原真菌具有拮抗作用的内生真菌,通过对峙试验,对筛选出的拮抗作用较强的4种内生真菌进行了鉴定和生物学特性研究,确定了不同内生真菌生长的最适培养基碳源温度和pH值,为更好地利用它们提供了最佳的培养条件:烟田杂草;内生真菌;生物学特性:S476+.9:A:1008-0457(2010)05-0461-05TheIsolationandCharacterizationofEndophyticFungifromWeedsinTobaccoFieldZHANGXiuwei,SANGWeijun*,XIEXin,WANGLishuang,GUIZewen(GuizhouKeyLaboratoryforPlantPestManagementofMountainousRegion,GuizhouUniversity,GuiyangGuizhou550025,China)Abstract:Endophyticfung,iwhichdemonstratedantagonismontobaccopathogenicfung,iwereseparatedfromtheweedsintobaccofields.Byconfrontationtests,thescreenedfourendophyticfung,iwhichshowedstrongantagonism,wereidentifiedandtheirbiologicalcharacteristicswerestudied.Thegrowthconditionsoftheendophyticfung,iincludingdifferentmedium,carbonsource,temperature,andpHvalueetc.werefurtheroptmiized.Keywords:weedsintobaccofields;Endophyte;biologicalcharacteristics,,,[1],,44Alternariaalternata(Fries)KeisslerThielaviopsisbasicola(Brek.etBr.)Fusariumoxysporum(Schlecht).fsp.nicotianae(Johns.)SnyderetHansen,4,11.1Conyzacanadensis(L.)CronqChrysanthemumindicumL.Galinsogaparvi山地农业生物学报29(5):461~465,2010JournalofMountainAgricultureandBiologyflora(Cav.)Camelliajaponica;-1.21.2.1内生真菌的分离,75%4min,3,2.5%3min,3,0.5mLPDA,,25[2]3~4d,,!∀[3]1.2.2拮抗作用内生真菌的生物学特性研究()()3d,pH,,3d[4-6]322.14,20(1),4,,1Tab.1Theendophyticfungiseparatedfromdifferentweedsintobaccofields83X1;X2;#X8X9;X10;X1132M1;M2;M3M4;M52L1;L22Y1;Y22.24!∀,4,22Tab.2Theidentificationresultsofendophyticfungiofweedsintobaccofields,,(AlternariaNeesexWallr.)X3,,,,,,;,,,,,,M3,,,(PenicilliumLK.exFries)L2,,,,,,,,,1,,,,,,,,,,,,(ArachniotusSchroter)Y1,,,,,(3.2-4∃2-2.5um):4462山地农业生物学报20102.32.3.1不同培养基对4种内生真菌生长速度和长势的影响3,4:X34,4:,1%3,;M3,,,,3;L2,1.55cm/d,3,,,;Y14,,1.421.37125cm/d,,,334Tab.3Theeffectsofdifferentmediaonthegrowthoffourendophyticfungi(cm/d)5%1%4X3,,,3.00aA,2.39bB,1.67cC,1.00dD4M31.38aA,,1.33aA,1.21aA,0.83bB4L2,,1.55aA,,1.28bB,,1.24bB,,1.07cB4Y1,,1.42aA,,1.37aA,,1.25aA,0.87bB2.3.2不同碳源对4种内生真菌生长速率和长势的影响4,4:X3,2.39cm/d1%,X3,;M3,3;L2,41%,,,3;Y1,2.3.3不同温度pH值对4种内生真菌生长的影响5,pH4:10,X3,25,,pH5~9,pH69;M310,,,302.06cm/d,pH4~10,pH7,1.34cm/d;L210,,25,pH4L2,5~9,pH7;Y110~30,25,pH69,5~10pH,6,4635,:44Tab.4Theeffectsofdifferentcarbonsourcesonthegrowthoffourendophyticfungi(cm/d)5%1%4X3,2.39aA,1.83bB,,1.44cC,1.42cC4M30.90aA0.66aAB0.71aAB0.47bB4L2,1.33aA,1.25abA,1.19bA,1.22bA4Y1,1.33aA,1.25abA,1.19bA,1.22bA54Tab.5Theeffectsofdifferenttemperaturesonthegrowthoffourendophyticfungi10(cm/d)15(cm/d)20(cm/d)25(cm/d)30(cm/d)X3%0.891.792.832.67M3%0.651.181.782.06L2%0.841.331.731.59Y10.451.041.281.681.616pH4Tab.6TheeffectsofdifferentpHvaluesonthegrowthoffourendophyticfungi4(cm/d)5(cm/d)6(cm/d)7(cm/d)8(cm/d)9(cm/d)10(cm/d)X30.420.820.940.760.580.930.73M30.611.251.281.341.081.360.98L2%0.540.420.660.310.10%Y1%0.631.040.990.841.141.0033.1,,,4,,;,;,,;M3,,3.24,4pH,:X3,pH6925;M3,pH7930;L2,pH925;Y1,4,pH9253.3,PDA,464山地农业生物学报2010;,,,,,:[1].Fusariumsp.Y2[J].,2010,269(4):249-253.[2],,.[J].,2007,19(1):98-100.[3],,,.[J].,2009,30(15):108-183.[4].[M].:,1974:405-602.[5],,.[J].,2007(1):47-49.[6],.[J].,2005,24(5):438-441.(455),,4.2,,,4.3,,,,:[1],,,.GIS[J].,2001(4):44-47,51.[2],,.GIS[J].:,2003,26(3):294-297.[3],,,.GIS%%%[J].,2006,15(3):325-329.4655,: