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311()Vol.31No.120103JournalofYangzhouUniversity(AgriculturalandLifeScienceEdition)Mar.20102史岩,秦爱建*(,225009):2(PCV2)PCV2,PCV2,;PCV22,ORF2,PCV2b1486TcAaacCCCGG,PCV2aAcCaacAAAAT;PCV2,PCV2,,,PCV2:2;;:S852.65+9.2:A:1671-4652(2010)01-0010-06Progressinbiologicalandpathogeniccharacteristicsofporcinecircovirustype2SHIYan,QINAijian(KeyLabofJiangsuProvVetMed,YangzhouUniv,Yangzhou225009,China)ABSTRACT:Thisreviewsummarizedthepathogenbiologicalandclinicalpathogenstudyinrecentyears.Thepathogenbiologicalstudyoftheporcinecircovirustype2(PCV2)indicatedthatPCV2couldinfectdifferentcellsindifferentagesofpig,anditcouldregulatethecytokinesecretioninmonocytesandmacrophagesalso.PCV2hadtwogenotypeswhichcouldbedistinguishedbythespecificnucleotidessequenceintheORF2frame.ThespecificnucleotidesinPCV2bwere1486TcAaacCCCGG,andAcCaacAAAATinPCV2agenotype.TherewasnohighidentitybetweendomesticandabroadisolatesbysequenceanalysiswhichsuggestedthatthePCV2hadexistedforalongtimeinourcountryandprobablyithadmutatedinsomenucleotides.Inthepathogenicstudy,thePCV2couldcausemultisystemicwastingsyndrome,sowreproductivefailureandporcinerespiratorydiseasecomplexbycoinfectionwithotherpathogens.KEYWORDS:porcinecircovirustype2;biologicalcharacteristic;pathogeniccharacteristicsTischer[1]1974PK15ATCCCCL31,,(CPE),,1982,Tischer[2],S1,,DNA,PCV(Porcinecircovirus),,Ellis[3](PMWS),PK15Allan[4]2(PCV2),PK151(PCV1)PMWS1986,1991[5],,,:2009-11-16:(07KJA23021):(1966-),,,,*,Email:aijian@yzu.edu.cnPMWS,PCV2,PMWS,PCV2(RF)(PRDC)(PNP),(PCVAD),,PCV21PCV2,;PCV;,,PCV2,77%~95%,26%~55%,Edwards(1994),44,86%PCV95%,5%~15%,40%,[6]7()22559,ELISA201~2,429%,PMWS[79],PCV,PCV2,,2PCV2PCV2,20,,17nm,CsCl137gcm-3ICTV(InternationalCommitteeoftheTaxonomyofviruses)6,PCV(CAV)(PBFDAV)31,PCV,7015min,56,PCV(PPV),PCV1PCV23PCV2PCVDNAPCV11759bp,7PCV23,176817671766bpPCV1PCV268%[10],PCV295%,PCV1PCV2,PCV2PCV22PCV2a(PCV21)PCV2b(PCV22)[11],95%,ORF290%;ORF2,PCV2b1486TcAaacCCCGG,PCV2aAcCaacAAAATPCV211,ORF1~ORF11,ORF1ORF5ORF7ORF105!3!,ORF2ORF3ORF4ORF6ORF8ORF9ORF115!3!,,PCV2ORF1945,314,PCV2ORF2705,234,ORF13,ORF2ORF4111ORF,ORF1ORF2,ORF2PCV2ORF2,[12][13],PCV2ORF2,ORF2400PCV2,PCV2111:265~87113~139157~183193~207C4[14],,,,B133PCV1PCV2[15]PCV1ORF2PCV2ORF2,ORF2PCV2Liu[16]ORF2MBPHis(8)tag3!,ORF2,PCV2,ORF2PCV1PCV2TruongORF2PCV,PCV2,PCV2ORF2,Cap12~1834~41[17],ORF2,ORF2[18]PCV2,PCV2PK15120,PCV2328(C328G)573(A573C)C328G(P110A),A573C(R191S),2,PK15[19]4PCV,PCV2,(DCs),80%~90%DCsPCV2,,,[20]PCV2IgMCD8CD4/CD8(DP)CD8CD4/CD8,CD8,DP,CD21CD3PCV227d;PCV2CD4CD8DPDP,NK,PCV221d,Shibahara[21]PCVB,,BCD8T,PCV2PCV2,IL10mRNA,IFNmRNA,IL2IL4IL12p40IFNIL10mRNA,IL8mRNA,PBMC,PCV2,IFNIL10[22]PCV2PBMC,PHAIL2IFNPBMC,mRNA,IL10mRNA,PCV2,,PCV2,,PCV2:Romeo[23],,PCV2;,;,,PCV2PCV212()31RF3:∀,;#;∃PCV2,PCV2PCV23:∀;#,;∃PCV23ISH,PCV2,,,5PCV251PMWSPCV2PCV2PPV,SPPV,PPVDNA,PCV2;2,PPV,,,PCV2PPVPRRSVPMWS,PCV2PRRSVPCV2PMWS,,,PCV2,PCV2/PRRSVPCV2,PRRSV,,PRRSVPCV2PCV2/PRRSV,,,PRRSVPCV2,NK,T,PCV2PRRSV,PCV2B,,;CSFV(),,,,,PMWS,PCV2PCV2,,PCV2PMWS,(PPVPRRSVCSFV)[24]52PCV2PCV2RF,PPVPRRSVCSFV(EMCV)RF:(),,30%~40%;RFCSFVPRRSVPPVPRVEMCV(JEV),,,6RF,PRVPCV2PPV[25]PCV2PPVPRRSVCSFVPRV4,PCV2EMCV,,,PCV2,,,PCV2[26]131:253PCV2PRDC,PCV2PRRSV(SIV)(PRV)PRDC8~26,,PCV2PRDC,PCV2,SIVPRRSVPRV(ISH)PRDCPCV2,PRRSVSIVPRV(PNP),PCV2(851%),PRRSV(446%),40%PRRSVPCV2SIVPRVPNP,PCV2[27]PRDC,IHC,PCV2,PRRSVPCV2,,PCV2,PRDC19%PCV2,PCV2355%,PRRSV/PCV2(519%)[28]Thacker[28],PCV2,,PRDC,,,PCV2PCV2,PCV253PCV2PCV2,E(HEV)(PEDV)PCV2(SAMS)PRRSV,,,PCV2PCV2/PPVPCV2/PRRSV,,,PCV2HEVPRVJung[29],PCV2PEDV,PCV2PEDV,,PCV2,PEDV,PEDV,PEDV,PEDV2,PCV2/PEDV,,(MFIN),Martnez[30],PCV2PRRSVPPV,,PCV2:[1]Tischer,RaschR,TochternannG,etal.Characterizationofpapovavirusandpieornavimslikeparticlesinpermanentpigkidneycelllines[J].AbtOrigA,1974,226:153167.[2]TischerI,GelderblomH,VettermannW.AverysmallporcineviruswithacircularsinglestrandedDNA[J].Nature,1982,295:6466.[3]EllisJL,HassardE,ClarkJ,etal.Isolationofcircovirusfromlesionsofpigswithpostweaningmultisystemicwastingsyndrome[J].CanVetJ,1998,39:4451.[4]AllanGM,MeehanD,ToddS,etal.Novelporcinecircovirusesfrompigswithwastingdiseasesyndromes[J].VetRec,1998,142:467468.[5]ChaeC.Areviewofporcinecircovirus2associatedsyndromesanddiseases[J].VetJ,2005,169:326336.[6],,,.[J].,2000(3):35.14()31[7],,,.2PCR[J].,2001,20(1):5356.[8],,,.SYBRGreenIPCR2[J].:,2007,28(3):1317.[9],,,.PCV2Cap[J].:,2008,29(2):15.[10]MamkertzA,CaliskanR,HattermannK,eta1.Molecularbiologyporcinecircovirus:analysesgeneexpressionandviraldiplication[J].VeterinaryMicrobiology,2004,98:8188.[11]OlveraA,CorteyM,SegalsJ,Molecularevolutionofporcinecircovirustype2genomes:phylogenyandclonality[J].Virology,2007,35(7):175185.[12]LiuQ,WillsonP,AttohpokuS,eta1.BacterialexpressionofanimmunologicallyreactivePCV2ORF2fusionprotein[J].ProExpPurif,2001,21:115120.[13]YunaK,JinhyunK.Characterizationoftherecombinantproteinofporcinecircovirustype2fieldisolateexpressedinthebaculovirussystem[J].VetSci,2002,3(1):1923.[14]HamelAL,LinLL,NayarGP.Nucleotidesequenceofporcinecircovirusassociatedwithpostwemfingmultisystemicwastingsyndromeinpigs[J].JViro1,1998,72:5262.[15]MaheD