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·2010254107-1102010－07－2670441975－。12121211231．4530032．4530033．1500015。10SPF。3H3N2。EID500－9.56/0.2mL10－8.56/0.2mL10－8.44/0.2mLLD5010－1.56/0.1mL10－1.45/0.1mL10－1.50/0.1mL1%、、、、、、、、O。。S432.4+1A1000－7091201004－0107－04IsolationIdentificationandBiologicalCharacterizationofSwineInfluenzaVirusesinHenanZHAOPu12ZHENGYu-shu12JIABei-bei12LIULi-yan1LIUXing-you12LIHai-yan31．DepartmentofAnimalScienceandTechnologyHenanInstituteofScienceandTechnologyXinxiang453003China2．ResearchCenterforAnimalDiseasesControlandResiduesSupervisionXinxiang453003China3．StateKeylaboratoryofVeterinaryBiotechnologyAnimalInfluenzaLaboratoryofMinistryofAgricultureHarbinVeterinaryResearchInstituteofChineseAcademyofAgricultureSciencesHarbin150001ChinaAbstractSampleswerecollectedbycottonswabsandlungtissuesfrompigswithrespiratorysystemproblemson5farmsinHenanprovince．Sampleswereinoculatedinto10-day-oldSPF-embryonatedeggsviatheallantoicrouteaf-terbeingprocessed．ThreeisolateswereobtainedandidentifiedasinfluenzaAvirussubtypeH3N2byHAandHI．Somebiologicalpropertiesofthemwerestudied．50%egginfectivedosesEID50were10－9.56/0.2mL10－8.56/0.2mLand10－8.44/0.2mL.50%lethaldosesLD50tosmallwhitemiceofthestrainwere10－1.56/0.1mL10－1.45/0.1mLand10－1.50/0.1mLrespectively．Hemagglutininsofthestrainswereunstabletoheat．RedbloodcellsofhorsecattlepigdonkeypigsheepcockrabbitguineapigsmallwhitemousebigwhitemouseandhumanO-typecouldbeagglutinatedbytheisolate．ItwilllayafoundationforunderstandinginfluenzavirusescirculatinginswineherdsinHenanprovince．KeywordsSwineinfluenzavirusIsolationIdentificationBiologicalpropertiesSwineinfluenzaSISwineinfluenzavirusSIV、。、、、1。SA-α-26GalAvianinfluenzavirusAIVSA-α-23GalAIV-“”23H1N14。10825。、。11．11．1．1H1、H3、H5H9、NDN1、N2。1．1．2SPFSPF。1．1．352320%～50%2000U、200μg、500μg、1000U、100U2。1．21．2．1SIV1．2．1．11000g10min1∶1031000g15min、1000U/mL4℃2h2－20℃。1．2．1．21．2．1．110SPF40.2mL/35℃4d。24h24～96h4℃－20℃1h1%3。1．2．21．2．2．110×10－4～10－9410SPF0.1mL/35℃4d24h。3。1．2．2．21．2．2．110－4510SPF0.2mL/35℃4d24h。1%8log2－70℃。1．2．2．3HI5100%。3log2。SIV。1．2．2．4NISIVNDVN1、N2NI637℃。50μL50μL、N1、N2PBS30min0.1mL37℃。0.1mL20min1mL2.5mL15min10min。1．2．3SIV1．2．3．1EID505SIV10×10－7～10－11410SPF0.2mL35℃4dReed-Muench7EID50。1．2．3．2LD501．2．3．110－1～10－550.1mL/1424hReed-Muench7LD50。1．2．3．34109110.5mL/56℃051015306090120150180210min4℃5～10min。1．2．3．4SIV1%、、、、、、、、、O。4log2“+”4log28“－”。22．12．1．1511%211ND。2．1．2H3H1、H2、H5、H9H3SIV。2．1．3N1、N2N2N1、PBSN2N1N2。2．22．2．1EID505SIV10×10SPFSWHN1SWHN2SWHN3EID5010－9.56/0.2mL10－8.56/0.2mL10－8.44/0.2mL。2．2．2LD505SIV10×SWHN1SWHN2SWHN3LD5010－1.56/0.1mL10－1.45/0.1mL10－1.50/0.1mL。2．2．356℃18。156℃Tab．1Thetiterofstraintobetestedunder56℃Strainname56℃/minWaterbathtimeunder56℃051015306090120150180210ThermalstabilitypeSWHN11085654442332SWHN21199887654220SWHN39766544543202．2．42。2、、、、、、、、O。2Tab．2ResultofagglutinationspectrumStrainname1%HorseCattleDonkeyPigRabbitCockSheepGuineapigSmallwhitemouseBigwhtiemouseOHumanO-typeSWHN1+++++++++++SWHN2+++++++++++SWHN3++++++++++++．1%－．1%。Note+．Agglutination1%RBCoftheanimal－．Notagglutination1%RBCoftheanimal．33．1。、“”、、、。。2d－70℃。。11H3N2SIV。110253．2。SIVAIV。SIV。EID50LD50。EID50、LD5014H3。3．3。。8。3．4SIV。SIV、、、、、、、、、O。1．———J．200844954－56．2ItoTCouceiroJKelmSetal．MolecularbasisforthegenerationinpigsofinfluenzaAviruswithpandemicpo-tentialJ．JVirol19987297367－7373．3KarasinAICarmanSOlsenCW．Identificationofhu-manH1N2andhuman-swinereassortantH1N2andH1N1influenzaAvirusesamongpigsinOntarioCanada2003to2005J．JClinMicrobiol20064431123－1126．4NaffakhNvanderWerfS．April2009Anoutbreakofswine-origininfluenzaAH1N1viruswithevidenceforhuman-to-humantransmissionJ．MicrobesInfect2009118/9725－728．5OlsenCWSCareyLHinshawetal．Virologicandse-rologicsurveillanceforhumanswineandavianinfluenzavirusinfectionsamongpigsinthenorth-centralUnitedStatesJ．ArchVirol200014571399－1419．6BrownIHChakravertyPHarrisPAetal．Diseaseout-breaksinpigsinGreatBritainduetoaninfluenzaAvirusofH1N2subtypeJ．VetRec199513613328－3297．M．1997109－119．8．SIVD．2002．