玉米小斑病菌生物学特性研究

玉米小斑病菌生物学特性研究谢红辉收稿日期:2010-09-03(广西壮族自治区亚热带作物研究所,南宁530001):通过研究不同培养温度不同培养pH值条件及不同营养条件对玉米小斑病病菌菌丝生长量及产孢量的影响结果表明:以PDA+VB1培养基培养的菌丝其生长速率及产孢量最大;菌丝在30!下生长速度快,生长量大,病菌孢子在25!下萌发速度最快;∀病菌菌丝在pH值为8时,生长速度最快,生长量也最大:玉米小斑病生物学特性(ZeamaysL),,,(Cornsouthernleafblight),,,,15%~20%,50%[1],1970,T,T,,165kg,15111A:PDA5mgVB1B:PDA,()200g20g20g,dH2O1000mLC:,30g20g,dH2O1000mLD:,100g17g,dH2O1000mLE:(),10g5g(MgSO4#7H2O)25g(FeCl3)002g50g20g,dH2O1000mLF:(),2g1g05g(MgSO4#7H2O)05g(FeSO4)001g30g20g,dH2O1000mL121216,5,5(90mm,)10mL,(05cm,)30!,,3d1,,10mL,(Neubauer),3122,PDA5!10!15!20!25!28!30!32!35!40!5,3d1,,120106(131)5L1∃105/mL10!15!20!25!28!30!32!35!40!45!,3h13,100,,,[2]123pH1mol/LHClNaOH(PDA,)pH2030405060708090100110120,250mL,100mL,121!20min,,4()30!110rpm7dpH3,7d,(),,:=--=/72211,ABCD4EF2A,PDA,,PDAVB11(d)(cm)ABCDEF337%006a34%005a31%013b32%010b12%004c13%005c679%006a67%015b60%023c62%007bc44%014d38%017e983%003a80%004b78%006c74%006c65%012d60%010e注:表中数据采用Duncan新复极差法进行统计检验表2表3同1,A,26∃104/mLB,EF,03∃104/mL07∃104/mL22,40!5!10~35!,25~35!,30!(9d84cm)9d,28~32!,,1cm,30!22,15~40!,25~35!,25!,9h62%,,,35!25!35!,,10!45!23pH3,pHpH8,,1840g0263g;1795g0257gPDA,pH65~80,2GUANGXITROPICALAGRICULTURE(GeneralSerialNo131)No6Nov2010122(d)(cm)10!15!20!25!28!30!32!35!306%005h12%004g16%002f24%005d35%008c40%004a35%009b17%005e609%011g21%005f27%006e40%007d64%023b75%033a66%023b47%008c910%014e29%009d39%006c68%013b83%004a84%007a82%012a69%022b3pHpH(g)(g)pH(g)(g)20069%0010e0485%0005e80263%0005a1840%0040a30075%0005e0525%0005e90128%0010b0895%0005b40121%0015d0845%0005d100079%0000d0556%0005d50125%0010d0875%0025d110073%0005e0510%0010e60127%0005c0890%0040c120006%0005f0045%0005f70176%0005b1235%0005b///3pHPDA,(2003)PDA,(,,;)[3],,PDA+VB1PDA2,,,PDA+VB1,&∋,320106(131)25~32!10~35!,28~30!;5~42!,26~32![3],,30!,,25!pHpH26~109[4]pH,pH8,,[1]陈利峰农业植物病理学[M]北京:中国农业出版社,2002:189[2]方中达植病研究法(第三版)[M]北京:中国农业出版社,1998[3]陈颖,郝丽梅,王立安不同培养基对玉米小斑病菌生长繁殖及致病力的影响[J]河北师范大学学报(自然科学版),2003,27(2):4143[4]赖传雅农业植物病理学(华南本)[M]北京:科学出版社,2003:9StudyontheBiologicalCharacteristicsofBipolarismaydisXieHonghui(SubtropicalCropsResearchInstituteofGuangxiZhuangAutonomousRegion,Nanning530001,China)Abstract:Theinfluenceofdifferentculturetemperature,differentpHvalueanddifferentnutrientsonthemyceliumgrowthandsporulationofBipolarismaydiswasstudiedinthispaperTheresultsshowedthat:OnPDA+VB1culturemedium,thegrowthrateandsporulationofmyceliumaremaximum;themyceliumhasfastgrowthrate,largegrowthincrementat30!,andthesporehasthefastestgerminationspeedat25!;∀themyceliumhasthehighestgrowthrateandthelargestgrowthincrementwhenitspHvalueis8Keywords:Bipolarismaydis;biologicalcharacteristics本刊重要启事经新闻出版总署批准(新出审字(2010)601号),∗广西热带农业+自2011年1月第1期(总第132期)起更名为∗农业研究与应用+新刊号为:CN45-1380/S,其他登记项目不变报道内容包括农业基础研究应用开发和理论探讨,涵盖选育种生物技术生态资源耕作植保土肥畜牧园林农产品检测加工贮藏农业经济等专业学科欢迎订阅!欢迎投稿!编辑部地址:广西南宁市邕武路22号,广西亚热带作物研究所内邮编:530001联系电话:0771-33488503348689投稿信箱:GXRZ@chinajournalnetcn二,一,年十一月十日4GUANGXITROPICALAGRICULTURE(GeneralSerialNo131)No6Nov2010