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3(,100084)(BOD),BOD5d,,BODBOD,BOD(BODst)BOD5BOD,,2003207218;2004206203(No.50327802)1,,,,(biochemicaloxygendemand,BOD),,1936,[13]BOD55d,,BODBOD,,BOD[48]BOD2BOD2.1(IUPAC):(),,,,1956Clark[9],1975YellowSpring,40,,,,12.2BOD,BOD,1977Karube[10]BOD,BOD3320053(FENXIHUAXUE)ChineseJournalofAnalyticalChemistry34054101Fig.1Schematicdiagramofbiosensorbasedondifferentprinci2plesBOD23[11],,Teflon,I0;,,,Teflon,,I1,,(I0),()BOD,BODBODI(I=I1-I0)BODst1520min,1560min,,(I/t),,1530s,2BOD[12]Fig.2SchematicdiagramofBODsensor10min,GGA(150mL/L150mL/L,20010mg/LBOD5)r=0.998,;[11]Liu[12],GGA,0.9811.0810.881,,2.3BOD2.3.1BOD(Arxualadeninivorans,BacilluspolymyxaD212,Bacillussubtillis,Hansenulaanomala,KlebsiellaoxytocaAS1,Pseudomonasputida,SerratiamarcescensLSY4,Toru2lopsiscandida,Trichosporoncutaneum),(BacillussubtilisBacilluslicheniformis7B,RhodococcuserythropolisIssatchenkiaorientalis),,,(),,BOD,,Tan[13,14]Gewaesserschntz[15],Bacillussubtilisg2802.5min604333BOD[13]Fig.3ResponsecurveofBODsensor,,2.3.2()BOD,(opticalfiber)(surfacepho2tovoltagedevice,SPV)Clark[9]Clark,,,,BOD,BODYang[16,17]Clark(15mm2mm0.4mm);(26mm76mm0.9mm)Chan[18](59mm7.5mm),3,PtAg/AgClPtBODPreininger[19](Tris(4,72diphenyl21,102phenanthroline)rutheniumCperorate)Li[20]BOD5Chee[21]Koenig[22]BODBODMurakami[23],BOD3BOD3.1BODBODBOD,BOD[24],,(),,,3.2BODBOD,Karube[10,25]BOD,(clostridiumbutyricum)(photobacteriumphosphoreum)BOD[25,26]Gewaesserschutz[15]Hassapis[27]BOD,(BIOX21010)STIPIsco7043:,BODpH(pH2ISFET)[28](SPV)[23][29]25min,14,5BODBOD,0100mg/L,,pH,pH,BOD5BODBOD,,,,Yoshida[30][HCFD],BOD15200mg/L,15minTrosok[31]BOD,BOD[3234]4BODBOD1983(NisshinDenki(Electric))BOD,1BOD1BODTable1Comparisonoftheanalyticalpropertiesforsomebiochemicaloxygendemand(BOD)devicesModelBOD22000BOD23000BODypointBIOX21010cRODTOX2000QBODmetre&EZ2BODmetreeRACODTMmetreManufacturerJapanNisshinDenki&CentalkagakuCo.Berlin,GermanyAucoteamGmbHGermanySTIPIsoGmbHGroB2UmstadtBelgiumKelmaUSABiosensorInc.USAUSFiltervinelandConfigurationBofilmflow2throughBofilmflow2throughBioreactorDynamicdilutionRespirometerBODBODandtoxicityanalyzerRespirometerRespirometer()(bioreactor)MicroorganismsT.cutaneumT.cutaneumC.parapsilosisBacteriaisolatedfromwastewaterActivatedsludgeActivatedsludgeActivatedsludge(min)Measuringtime20401315204020d,1560c10H30L(mg/LBOD)Measuringranges010002000500a31000b550051500,2015002010000005000000.53000.5500d1004000H0100L(d)Workingstability30a,b30146090d(%)Precision3a,b103510((low))d,5((high))dStandardsolutionGGAGGAsolutionGlucosesolutionEffluentfromwastewatertreatmentplantReferences[37,38][37,38][31][39]()()a.BOD22000;b.BOD23000;c.(measuringprinciplebasedondynamicdilution);d.OBOD(metre);e.EZ2BOD(metre);H:(highconcentrationrange);L:(lowconcentrationrange),SXI2VBOD,BODBOD5,,BOD80433BOD5[3840]BOD,,5BOD,BOD,BOD,BOD,,BOD,,:(1);(2);(3);(4);(5),BODst,,BODBOD,,BOD,BODReferences1WangJianlong(),ZhangYue(),ShiHanchang(),QianYi().BiotechnologyBulletin(),2000,3:13192WangJianlong(),WenXianghua().ModernEnvironmentalBiotechnology().Beijing(),TsinghuaUniversityPress(),20013WangJianlong().MicrobialImmobilizationTechniquesandWaterPollutionControl().Beijing(),SciencePress(),20024ZhangYue(),WangJianlong(),LiHuazi().HighTechnologyLetter(),2001,8:37395LiHuazi(),WangJianlong(),ZhangYue().HighTechnologyLetter(),2002,3:88906ZhangYue(),WangJianlong(),LiHuazi().HighTechnologyLetter(),2001,8:55577ZhangYue(),WangJianlong(),LiHuazi().MarineEnvironmentalScience(),2001,20(1):51548WangJianlong().ProgressinBiochemistryandBiophysics(),2001,28(1):1251289ClarkLC.Jnr.Trans.Am.Soc.Artif.Intern.Organs.,1956,2:414810KarubeI,MatsunagaT,MitsudaS,SuzukiS.Biotechnol.Bioeng.,1977,19(10):1535134711YangZ,SasakiS,KarubeI,SuzukiH.Anal.Chim.Acta,1997,357(122):414912LiuJ,MattiassonB.WaterResearch,2002,36:3786380213TanTC,QianZR.SensorsAcutatorsB,1997,40:657014QianZ,TanTC.WaterRes.,1998,32(3):80180715GewaesserschutzTM,Wasser,Abwasser,1988,105:18319516YangZ,SuzukiH,SasakiS,KarubeI.Appl.Microbiol.Biotechnol.,1996,46(1):101417YangZ,SuzukiH,SasakiS,McNivenS,KarubeI.SensorsAcutatorsB,1997,45:21722218ChanC,LehmannM,ChanK,ChanP,ChanC,GruendigB,KunzeG,RennebergR.Biosens.Bioelectron.,2000,15:34335319PreiningerC,KlimantI,WolfbeisOS.Anal.Chem.,1994,66(11):1841184620LiXM,RuanFC,NgWY,WongKY.SensorsAcutatorsB,1994,21:14314921CheeG2J,NomuraY,IkebukuroK,KarubeI.Biosens.Bioelectron.,2000,15:37137622KoenigA,BachmannTT,MetzgerJW,SchmidRD.Appl.Microbiol.Biotechnol.,1999,51:11211723MurakamiY,KikuchiT,YamamuraA,SakaguchiT,YokoyamaK,ItoY,TakiueM,UchidaH,KatsubeT,TamiyaE.SensorsActuatorsB,1998,53:1631729043:24SpanjersH,VanrolleghemP,OlssonG,DoldP.WaterSci.Technol,1996,34(324):11712625KarubeI,MatsunagaT,TsuruS,SuzukiS.Biotechnol.Bioeng.,1977,19:1727173326KarubeI,MatsunagaT,SuzukiS.J.FermentTechnol.,1977,55(3):24324827HassapisG.IEEETrans.Instrum.Meas.,1991,40(6):1015102028MitsubishiElectricCorp,Japan.Japanesepatent:BODMeasuringApparatus.JP58221158,198329IranpourR,StraubB,JugoT.J.Environ.Eng.,1997,123(2):15415930YoshidaN,YanoK,MoritaT,McNi