生物化学知识点习题答案

20R20R20RR8R7R2R3pKD-L2L280nm280nm12SangerNH22,4DNB3EdmanNH2PITSangerEdmenN231010NC,3.60.54nm,0.35nm,RR1162C321.1248mol/L2O2RpHpIpHpIpHpI12SDS-SDSSDSSDS3SSS)V1(DRTSM-=1dipolarion2essentialaminoacid3(isoelectricpoint,pI)4(rareaminoacid)5(nonproteinaminoacid)6(configuration)7(proteinprimarystructure)8(conformation)9(proteinsecondarystructure)10(domain)11(proteintertiarystructure)12(hydrogenbond)13(proteinquaternarystructure)14(ionicbond)15(super-secondarystructure)16(hydrophobicbond)19(saltingin)20(denaturation)21(renaturation)22(precipitation)23gelelectrophoresis24chromatography1__________2___%110mg,____%320_________________2___________________________.4______________________________3280nm5pI10.76,pH7_______620____________________________7___________________________8________________________,___________________9________10____________11______________12________________________________________________________________.13________________________________________14GSH__________________________________________15________________________________,_______,_____________16pH__________________________________________17________18_________________________19_______________________________________208.04.510.0,pH8.0______________________21pH=pI_____pHpI_______22pK1(-COOH)2.19,pK2(-NH+3)=9.67,pKR(R)=4.25,__________23_____24a90000b45000c110000_____________250.34%______.0.34%4________.26180__________.1pHABCDE2ABCDE3280nmABCD44ABCD5ABCD6ABC3.6D0.15nm.7ABCD8ALeuBAlaCGlyDSerEVal9ABCD10ABCD11ApHBCD12ABCD13ABCDE14ABCD0.35nm15ABCD16ABCDE17ABCD18ABCD19ABCDpH20ABCDE21ABCABDE22ABCDA,BC()1()2()3()4()5DL()6()720()8()9pICOOHNH2()10()11()12()13.FDNBEdmanN-()1420-()15R()16()17()18()19()20()21()22()23()24123456789CNBr6mol/LHCl-12344510151015Ile-Ala-His-Thr-Tyr-Gly-Pro-Glu-Ala-Ala-Met-Cys-Lys-Try-Glu-Ala-Gln-202527Pro-Asp-Gly-Met-Glu-Cys-Ala-Phe-His-Arg1-23123.pHpI42056789101112N-HO-H13141516171819202122232412166.25345678C=ON=H0.54nm3.60.15nm9101112131415161718FDNB2,4Edman19SDSSDS-PAGE2021223.222324Cab251667266687265027nm1D59.74,pH2B83A280nm540120,215nm,280nm215nm4D4R3R5B6B3.60.54nm,0.15nm,B7C8C-429B-NH10D11ApHpH12A13E,C=O-;14CC0.35nm,15C16E22217A18A19ApH20B21EpH22BB120.132nmCNCN34CC520LD2622788-R9pK1011124413EdmanNNEdmanNFDNBSangerNFDNB2,4DNPNNDNP-DNP-N1415RH+,H+,RH+16171819202122232412313.60.54nm,0.15nm.2NHCO3-4120.35nm0.325nm35612378123456789109abc-deCNBr;f101719213243AspGlnLysTryLeuValThrDNARNADNADNARNARNARNADNA2RNADNAcAMPcGMPDNA1953WatsonCrickDNAChargaffDNAA=TG¡ÔCDNAX-DNA3,5Watson-CrickDNA2nm0.34nm3610A=TG¡ÔCDNADNABDNAAZABDNADNAH-DNARNARNAtRNALmRNADNARNA2’-3’-DNA260nm260nm280nmA260TmDNAG+CTmGCATGCDNAATDNAxG+C=Tm-69.32.44DNATmG+CG+CTmDNADNADNARNARNADNADNADNADNADNA[]NADNADNADNADNADNADNA8H2AH2BH3H4DNA146bp1(mononucleotide)2phosphodiesterbonds3dissymmetryratio4(complementarybasepairing)5anticodon6cistron7denaturationrenaturation8annealing9hyperchromiceffect10hypochromiceffect11phage12hairpinstructure13DNAmeltingtemperatureTm14molecularhybridization15(cyclicnucleotide)1DNA_____________2_____3______4DNA____RNA____5_______________6____7C-C______8DNA____RNA_____9DNA____RNA_____10DNA______113H_______DNARNA12BDNA_________13DNAG-C___Tm___14___15____RNA_____RNA16DNA__________17DNA____________18_________nm19DNApH2pH12OD260______DNAOD260______20DNA______21DNA______DNA__________mol/LNaCI22mRNA___RNA________________23DNA_____________________24DNA_____DNA________________25mRNA______3______26_______________-OH27mRNA___________________28DNADNA____DNA___1ATPAR-A-P-P-PBA-R-P-P-PCP-A-R-P-PDP-R-A-P-P2hnRNARNAAtRNABrRNACmRNADSnRNA3tRNAA–XCCA3`BTCCDHUDEWatson-CrickDNA:A25400B2540C29411D2941E35055A23B24C25D34E356TAGApAAGATpBATCTpCTCTApDUAUAp7ADNABDNACtRNADmRNA8mRNAAm7APPPNmPNmPBm7GPPPNmPNmPCm7UPPPNmPNmPDm7CPPPNmPNmPEm7TPPPNmPNmP9DNAA260nmBCD10DNATmAA+GBC+TCA+TDG+CEA+C11GAACAUBUGCCCGUDUACE12mRNAm7GA2-5B3-5C3-3D5-5E3-313pH3.5AAMPBGMPCCMPDUMP14AcAMPcGMPBcAMPcGMPCcAMPDcAMP15DNAAH1H2H3H4BH1AH1BH2BH2ACH2AH2BH3AH3BDH2AH2BH3H4EH2AH2BH4AH4B1DNARNA23’3DNA4pH5DNA6tRNA7DNATmATATTm8mRNA5`A9DNATmA+T/G+C10B-DNADNAAZ11DNATm20122’3’-13DNA14mRNARNA15tRNAtRNA16DNAOD260/OD2801.8RNA1718ABAOD260/OD280BOD260/OD280AB19DNA20mRNA3’-OH1?DNARNA21T7DNA2.5107DNA6502130106DNA388tRNA4C10459.6mRNA1203DNAA+G/T+C=0.71A+G/T+C=2DNAA+G/T+C=3A+T/G+C=0.7A+T/G+C=4DNAA+T/G+C=4DNATm5pH7.00.165mol/LNaClDNATm89.36DNA12Tm2DNA78DNA9DNA10tRNA111mol/LKOHDNARNA12DNARNA13pH7.01.0cm260nmAAMPA260=15400GMPA260=11700CMPA260=7500UMPA260=9900dTMPA260=9200132mol/LAMP247.5mol/LCMP36.0mol/LUMP448mol/LAMP32mol/LUMPA260(5)A260=0.325GMP(/pH7.0)(6)A260=0.090dTMP(/pH7.0)141014DNA6.4109DNA-2.210915pH2.5pH3.5pH6pH8pH11.41pH2pH3pH1.(mononucleotide)2.phosphodiesterbonds3.dissymmetryratioA+T/G+C4.(complementarybasepairing)GCCGATTA5.anticodontRNAmRNA6.cistron:7.denaturationrenaturationDNADNADNADNA8.annealingDNA9.hyperchromiceffectDNA260nm10.hypochromiceffectDNA260nmDNA260nm35%~40%,11.phage12.hairpinstructureRNARNA13.DNATmDNATm14.molecularhybridizationDNADNARNA15.(cyclicnucleotide)3’-OH5’-OH1.Watson-Crick;19532.3.2’4.5.6.7.8.9.10.11.12