白头翁黑粉病菌生物学特性研究

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:2010-01-22:(sk2007005):(1958-),,,,。,2010-04,41(2):203-205JournalofShenyangAgriculturalUniversity,2010-04,41(2):203-205,,,,,,(,,110866):[Pulsitillachinensis(Bge.)Regel]。(Urocystispulsatillae(F.Bubak)Moesz)。:,。20~25℃,pH4~7,,56℃。:;;:S435.672:A:1000-1700(2010)02-0203-03BiologicalCharacteristicsoftheWindflowerSmut(Urocystispulsatillae)FUJun-fan,SUDan,ZHOURu-jun,YUShu-yi,YANXue-rui,LINXiao-yue,SHIJian-hua(CollegeofPlantProtection,ShenyangAgriculturalUniversity,Shenyang110866,China)Abstract:WindflowersmutcausedbyUrocystispulsatillaehasbecomemoreprevalentthroughoutthehillwindflowergrowingareaintheeastofLiaoningprovince.ThestudyonthebiologicalcharacteristicsofthepathogenshowedthattheoptimaltemperatureandpHforitsteliosporesgerminationwere20~25℃and4~7,respectively.Sucroseandasparatatewerethebestcarbonandnitrogensourcesforteliosporesgermination.Thelethaltemperatureofteliosporeswas56℃.Keywords:windflowersmut;Urocystispulsatillae;biologicalcharacteristics[Pulsitillachinensis(Bge.)Regel],、、,,,,,[1]。,,,。、,、,,,。(2007)[2]。KALMANVANKY[Urocystispulsatillae(F.Bubak)Moesz][3],。,,,。11.1。1.2,,。15050。,1/2[4]。1.31.3.1,10,15,20,25,28,30℃,1,2,3d,3。1.3.2pH1mol·L-11mol·L-1pH2,3,4,5,6,7,8,9,10,4111,12,KH2PO4,25℃,48h,,3。1.3.37(1%、1%、1%、1%D-、1%D-、1%、1%D-)7(1%、1%、1%、1%、1%、1%、1%),,25℃。48h,3。1.3.41%、、、、,,25℃,24h,3。1.3.520073,,,2009620,,。1%,25℃,24h,3。1.3.650g·L-12mL,50,51,52,53,54,55,56,57,58,59,60℃10min,24h,。22.11~6,,,,(1)。(Urocystispulsatillae),,。2.22:。5~30℃,;5~25℃,;25~30℃,。20~25℃,25℃,31%。2.3pH1,pH,。pH2~10,pH10,。pH2~6,pH;pH6~102Figure2Effectoftemperatureonteliosporesgermination1(A)(B)Figure1Morphologyofteliospores(A)andgerminatingteliospores(B)ofUrocystispulsatillaeAB204··2:,pH。pH6,14%,,。2.42,,16%,;、、D-。,27%,;,,。2.53,、、,,44%,。2.6(4),10d3%,,,2,24%。3Table3EffectofchemicalsonteliosporesgerminationChemicalsAscorbicIAAGACKNaOHH2O2/%Germinationrate44.0aA26.0bB14.6cC7.0dD0.0eE0.0eE/y-m-dCollectiontime2007-08-192008-05-132008-10-142009-05-102009-06-10/dTCT603393270399/%Germinationrate24.0aA20.7aA12.3bB8.3cB3.0dC4Table4Thegerminationpercentageofteliosporesindifferentperiods2.750~55℃10min,56~60℃10min。,56℃。3,20~25℃,pH4~7,,,,。30d,3%。,,,。:[1],,,.[J].,2004,13(4):160-164.[2],,,.2007[C]//:,2007:222-224.[3]KALMANVANKY.EuropeanSmutFungi[M].NewYork:FischerVerlagStuttgart,1994.[4]..[M].:,1979.[]pH234567/%Germinationrate1.63.49.610.814.39.60.05gecbac0.01GECBACpH89101112/%Germinationrate8.02.30.6000.05dfhii0.01DFHIISignificanttestSignificanttest1pHTable1EffectofpHonteliosporesgerminationCarbonsourceSucroseLactoseD-D-GalactoseD-D-MannoseD-D-XyloseAmylaseCKGlucose/%Germinationrate16.0a13.0ab10.6bc10.0bc8.3c7.3c7.3c7.0cNitrogensourceAspartatePeptoneCornflourAmmoniumoxalateAmmoniumchlorideUreaCKTyrosine/%Germinationrate27.0a18.6b17.3bc15.6bc14.6cd11.3de9.6ef6.6f2、Table2Effectofdifferentcarbonandnitrogensourcesonteliosporesgermination205··

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