石油微生物特性的实验研究

:1000-2634(2007)06-0058-053,,,,(,610500):,:,(›5)(œ75),,;;,,,,,,:;;;;:TE357.9:A(MEOR),,MEOR,,(),[1],,,[2],,,,MEOR,2[1,3],,;,()()[4]12550,(75)2,75,2,(1),(PAM)1.1,,,(),296Vol.29No.6200712JournalofSouthwestPetroleumUniversityDec.20073:2006-10-19:(20040615002);(SZD0414):(1961-),(),,,,11.2/:[4],,3:[5](1102mN/m),(10-1102mN/m),,1.3,,,,,01003,,,200,20,70%95[6],,4152530405060748,ABC123458,(),,pH8,,24h,,,1.4Collins(1970),(mg/L)Li+400Ba2+670B3+450Br-6000I-1400,,90%[7],22.1956:[7]1,8QZ22,C30,QZ22Dg-1,,,,4B;,,,,Dg-1QZ22112345ABC5%++++++++++++++++++QZ22+++++++++++++Dg-1----+++-C30--------++-+-++++:+++,++,+,-2.1.1B,BQZ22,2%+5%QZ22,,22/h246810121416182022242630/OD0.280.350.450.570.660.770.890.951.021.101.131.141.141.15/ml0.01.03.08.017.027.035.043.048.052.055.056.056.556.5:120ml+2%+5%QZ22+2mlB,=100%,2%+5%QZ22,B47.08%2h,1018h,26h,,2.1.2B,,,,33+/%63.087.6627.290.000.110.600.400.400.150.270.12,,N2O2CO278.00%21.00%0.03%0.07%;,,;,;0.03%33.29%;,062007[6],,[8],CO2;C10,(36.1)(29.9)[9],,,,,,,,CO2,,2.24,5,18.22%31.09%,1,2,,,,135BC412345/%76.777.277.577.377.677.3/61.461.860.959.960.260.8/142.341.541.842.641.341.931.09264.364.065.464.365.264.6-6.25342.344.343.344.443.643.628.29463.362.562.062.162.962.6-2.96551.749.549.148.749.249.618.22A61.459.060.061.159.460.20.99B48.347.346.946.846.347.122.53C45.544.644.944.845.145.025.99:=(m-n)/m,,m,mNm-1;n,mNm-12.322,,,,,;224,,B13,,24,8355///A51028405060B4825435060C10153542546011520354865702202530405560361035506065406253850605101535455060166:2.433ABC13NaCl2%8%,02%8%12%,NaCl12%,;C4,NaCl012%,NaCl12%,;5,NaCl2%8%,8%,33(1),;(2),,CO2;18%31%,;(3);,,,;;(4)NaCl:[,:95()(J1405)];,!:[1].[R].:,1989.[2],.[J].,2000,22(3):44-46.[3],,,.[J].,1996,13(2):157-161.[4]EC.[M].,.:,1995.[5].[M].:,1994.[6].[M].:,1992.[7]DuvnjalZ,CooperDG,LosaricN.EffectofnitrogensourcesonsurfactantproductionbyArthrobacterparaffineusATCC19558[C]//MicrobialEnhancedOilRecovery,1983:66-72.[8][]RM.[M]..:,1991.[9]HeinritzBM,Gehrhardt,BaumannF,etal.Biomasspro2ductionbythermophilicmicroorganismssimultaneouslyusingreactionheat[J].ChemistryTechologyBiotechol,1990,49:285-295.(::)262007natedcoccolithlimestoneandmudstonecanreach16.0%,thepermeabilityis(1100)10-3m2.So,coccolithlime2stoneisofgoodreservoirproperty.Theinterlaminatedcoccolithlimestoneandmudstoneispotentialoilreservoir.Keywords:QikouSag;Es1Formation;interlaminatedcoccolithlimestoneandmudstone;coccolithlimestone;reser2voircapacityEXPERIMENTSTUDYONCHARACTERISTICSOFOILMICROBESZHANGTingOshan,CHENXiaoOhui,JIANGZhaoOyong,LANGuangOzhi,HUANGShiOwei(FacultyofResourcesandEnvironment,SouthwestPetroleumUniversity,ChengduSichuan610500,China).JOURNALOFSOUTHWESTPETROLEUMUNIVERSITY,VOL.29,NO.6,58-62,2007(ISSN1000-2634,INCHINESE)Abstract:Themicrobeassociationgasgeneratingability,systemsurfacetensionofsurfactantgeneratedbymicrobe,heatOresistanceandsalyOtoleranceareanalyzed,theassociationisreperatedandselectedfromoilcontainingpollu2tedwaterfromQinhai,Dagang,Shengli,Liaohe,XinjiangandNanchongrefinery.Itisrecognizedthattemperatureinfluencesthemicrobegrowingandthemetablismofmicrobegroupgreatly,undertheconditionofextremetempera2tureoflowtemperature(5)andhightemperature(75),themicrobegroupislessactive,thegasgeneratingmicrobegeneratesnogas.Thechangeoftemperatureinfluencealsotheshape,sizeanddistributionofsinglemi2crobe,inhighsalinity,somemicrobecangrow.Theinfluenceofrelativemicrobecontentandinternalcompositionisanalyzed.Resultshowsunderacertaincondition,themicrobeassociationisofstrongergasOgeneratingability,itcandecreasesurfacetensionofthesystem,heatOresistanceandsaltOtolerance.Thesortedoilmicrobecanfairlya2dopttoreservoirconditionandreduceoilviscosity.Themicrobeassociationisofsuperiorityinoilrecovery.Keywords:characteristicsofoilmicrobes;heatOresistance;saltOtolerance;surfacetension;MEORMUDSTONECAPROCKSSEALINGMECHANISMOFBIOGENETICGASRESERVOIROFQUA2TERNARYINQAIDAMBASINWANGJinOpeng1,PENGShiOmi1,GUANZhiOqiang2,JIANGGuiOfeng2,LIUYuOkui3(1.FacultyofResourcesandInformationTechnology,ChinaUniversityofPetroleum,ChangpingBeijing102249,China;2.ResearchInstituteofExplorationandDevelopmentofQinghaiOilfieldCompanyofPetrochina,DunhuangGansu736202,China;3.Explo2rationDepartmentofTarimOilfieldCompanyofPetrochina,KuerleXinjiang841000,China).JOURNALOFSOUTHWESTPETROLEUMUNIVERSITY,VOL.29,NO.6,63-67,2007(ISSN1000-2634,INCHINESE)Abstract:ThemudstonecaprocksofbiogeneticgasreservoirofQuaternaryinQaidamBasinisintheearlystageofdiagenesis,withthecharacteristicsofpoorconsolidation,relativelyhighporosity,bigporediameterandrelativelylowerbreakthroughpressure,whichresultsintheweaksealingofbiogeneticgasreservoirs.TheremustbespecialsealingmechanismtoformlargescalegasObearingprovince.ThroughanalysisofpetrologyandmicroOstructurecharacteristicsofmudstone,itisindicatedinthispaperthatthecaprockqualityisthemainreasonthatformsweaksealingstateiftherearesufficientgasresourceandnofaultdestruction.Inthiskindofgeologiccondition,itcanbeconcludedthatthephysicalpropertiesbetweenmudstoneandsandstonearethefundamentalsealingconditiontopreventandalleviatebiogeneticg