离子束对生物体的作用原理及应用

2010294761767GenomicsandAppliedBiology,2010,Vol.29,No.4,761767ReviewandProgress1*21341,,010010;2,,017000;3,,010020;4,,021400*,[e-mail]4、。80。、。,,,TheActionPrincipleofOrganismsbyIonBeamandTheirApplicationXieJihong1*BaoJingang2XuZhu1YangKai3YinJun41GrasslandResearchInstitute,ChineseAcademyofAgriculturalScience,Huhhot,010010;2ErdosForestProjectStation,Erdos,017000;3InnerMongoliaGrasslandManagementStation,Huhhot,010020;4ManzhouliManagementBureauofMunlclpalPublicUtilities,Manzhouli,021400*Correspondingauthor,[e-mail]:10.3969/gab.029.000761AbstractIonbeammeansabunchofelectricallychargedionbeamwhichhasthemassnumberlessthanorequalto4withsomeenergy.Ionbeamimplantationtechnique,abiophysicaltechnology,hasthecharacteristicsofslightphys-iologyinjury,widemutationspectrum,highmutationfrequencyandsoon.Theinteractionbetweenionbeamandor-ganismisabiophysicaltechnologypossessingaindependentcopy-rightedproductinChina,theChinesescientistshavediscoveredthebiologicaleffectofionbeamimplantationinthe1980s,andappliedthisprincipleintoplantmuta-tionbreeding.Inthisarticle,wemainlysummarizedtheactionprincipleoforganismsbyionbeamanditsapplication,includingonplantbreeding,microorganismvarietiesimprovementandgeneticmodification,inconclusion,westillsumeduptheproblemsexistinginthisinvestigationfieldbyionbeamimplantationtechniqueandpointedoutout-looksforitsfuturedevelopmentorientation.KeywordsIonbeam,Biologicaleffect,Geneticmodification,Application()(2006104)“”“”(2008BADB3B04)4。、、、、。1986、、(,2004)、、“”()(,2005,,20(6):520-523)。20、、、、(Yu,2000)GenomicsandAppliedBiology(,1999)。、。1。(Bragg)、(LET)、。、、LETBragg。、、。、、4、、10-19~l0-16s(,2004)。。Huang(1998)N+α。(,2009)。(,2004)。。。。。。(1998)、(2003)α30keVN+1017ions/cm27.5~9.1μm。3。。。、(,2001)。(,2009)。、。、。。。。DNA、、、、、(,2009)。、DNA。(WuandYu,2001)。3(,1998)。22.13000、、、、(,1998)。1986(,2007)7S9042、D9055、63、M3122、14、48M1148。、4(,1994)2。(1993)、、。(1994)30KeV60CoγM1、M2。M1N+4×106ions/cm2、、。(HIRFL)、、、M920、、2(,2005,,19(1):80)“2”、、、、、667m2600kg20061.3×105hm2(,2008)。2006C、24%667m210tM2。(1995)。M1M1。(1994,:,6:22-24)M1(56.55%)(37.35%)19.20%。(2000,,(4):8-11)18.4%、。(2005)、、。(2006)、、、。15keVN＋44.1%(P＜0.01)(r=0.4537,P＜0.1)。20keVN＋31.8%(P＜0.01)。2.2。1994(,2007)。TheActionPrincipleofOrganismsbyIonBeamandTheirApplication763GenomicsandAppliedBiology。(1998)55%~60%19983。(1995)、。(2001)N+27.39%。(1999)H+、N+Ar+VcH+、N+Ar+3Vc4()80%94%。、。(2003a;2003b)(AA)33.8%AA52.36%126.2%2.5。2.32080、、(Yu,2006)。。、、、、。、。DNA。(2001)(1)。DNA。(2)。。(3)DNA。DNA。(4)。DNADNADNA。(5)。。(6)DNA。DNADNA(,2007)。。3、DNA(Yu,2006)。。Yu(1993)GUSPCRSouthernGUS。(1994)(2001)31%~2%。DNADNA。(2003)DNA、DNA18%M1、M2M3。(2003)(Lansberg)、、DNA(Columbia)2。(2000)DNA25%。(2001,,(2):2-3)DNA。32080、、。。、、、。BianP.,SuM.J.,QinG.Y.,YuZ.L.,andHuoY.P.,2003,ThemolecularanalysisofcabbagetotalDNAtransformationin-toA.thalianamediatedbykeyionbeam,ShengwuWuliXuebao(ActaBiophysicaSinica),19(3):327-332(,,,,,2003,DNA,,19(3):327-332)ChengB.J.,LiZ.,WangG.M.,LinY.,YuZ.L.,andHeJ.J.,1993,Themutageniceffectsofnitrogenionimplantationoncottonseed,HeNongxuebao(ActaAgriculturalNucleataeSinica),7(2):73-80(,,,,,,1993,,7(2):73-80)ChengB.J.,LiZ.,TianQ.Y.,WangG.M.,LinY.,YuZ.L.,andHeJ.J.,1994,Effectofnitrogenionimplantationonmor-phology,viabilityandfertilityofcottonpollen,XibeiZhiwuXuebao(ActaBotanicaBoreali-OccidentaliaSinica),14(2):85-89(,,,,,,,1994,,,14(2):85-89)HanJ.W.,andYuZ.L.,1998,Studyonetchingandpenetratingoflowenergyionsintotomatopeel,ShengwuWuliXuebao(ActaBiophysicaSinica),14(4):757-761(,,1998,,,14(4):757-761)HuangW.D.,YuZ.L.,andZhangY.H.,1998,ReactionsofsolidglycineincludedbykeVionirradiation,ChemicalPhysics,237(1-2):223-23lHuangG.W.,MaoP.H.,JinX.,andLvJ.,2009,Principleofex-ogenousMega-DNAtransformationmediatedbylow—en-ergyionimplantationandtheirapplications,JiyinzuxueYuYingyongShengwuxue(GenomicsandAppliedBiology),28(4):815-820(,,,,2009,DNA,,28(4):815-820)LiuS.Q.,JiaJ.M.,CaoP.,YangJ.C.,ZhangB.S.,LiuJ.R.,GaoP.,andShiW.D.,1995,Preliminarystudyoneffectsofni-trogenionimplantationoncorn(Zeamays)somaticcellcul-ture,HeJishu(NuclearTechniques),18(7):410-414(,,,,,,,,1995,,,18(7):410-414)LiH.,WuL.F.,andYuZ.L.,2001,Studyonricetransformationmediatedbylowenergyionbeamimplantation,HeNongx-uebao(ActaAgriculturaeNucleataeSinica),15(4):199-206(,,,2001,,,15(4):199-206)LiR.M.,WangJ.F.,andLiW.J.,2007,Applicationofheavyionbeamstomicrobialmutationbreedingandexploitationofbiologicalenergy,YuanziheWuliPinglun(NuclearPhysicsReview),24(3):234-237(,,,2007,,,24(3):234-237)MaH.P.,ZhaoZ.W.,JiangZ.K.,andKongC.J.,2003,Analysisofqualityandagronomiccharactersofwheatoffspringde-TheActionPrincipleofOrganismsbyIonBeamandTheirApplication765GenomicsandAppliedBiologyvelopedbyionbeammediatedtransferringsoybeanDNAintowheat,HenanNongyeKexue(JournalofHenanAgri-culturalSciences),(7):4-6(,,,,2003,DNA,,(7):4-6)QuY.,LiW.J.,ZhouL.B.,WangZ.Z.,DongX.C.,YuL.X.,LiuQ.F.,andHeJ.Y.,2007,Researchandapplicationofmutageniceff