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46820108SCIENTIASILVAESINICAEVol.46No.8Aug.2010*1321131111.4101282.4101283.410128‘’3、、。1‘’3、、、233、44353、2。S722.3+1A1001－7488201008－0056－062009－08－102009－11－01。04JJ302407CY001。*。BiologicalCharacteristicsofVariegatedBudSportsofLoropetalumchinensevar.rubrumLiYanlin13XiongXingyao2YuXiaoying1HeChangzheng13LüChangping1YuanFeirong1ZhuJiehui11.CollegeofHorticultureandLandscapeHunanAgriculturalUniversityChangsha4101282.ProvincialKeyLaboratoryofCropGermplasmInnovationandUtilizationHunanAgriculturalUniversityChangsha4101283.HunanProvincialEngineeringResearchCenterforPotatoesChangsha410128AbstractTheappearanceofvariednessandbiologicalcharacteristicsofthreevariegatedsportvarietiesandtheirmotherplantofLoropetalumchinensevar.rubrum‘MizhiMeihong’werecomparedwithvirologicalidentificationanatomyofbladecontentofpigmentsandphotosynthetictraits.TheleafextractsfromtheplantswereusedtoinoculateGlycinemaxCucumissativusCapsicumannuumSolanumlycopersicumL.chinensevar.rubrum.Theresultsshowedthattheinoculatedplantsgrewwellanddidnotexhibitanyvirussymptom.NoviralgranulewasdetectedbyTEM.ThebudsportvarietieswereabletomaintaintheirtraitofvariegatedcolorbycuttingpropagationandtheyhadhigherresistancetoplantdiseasesandinsectpestsandweresuitabletogrowinginfieldinHunanProvince.Therewasremarkabledifferenceinthicknessofleavesupperepidermisandlowerepidermisamong4plantspecimens.Thecontentsofcarotenoidandanthocyaninofvariegatedsportleavesweredistinctdissimilaramong3sportvarietiesandthenetphotosyntheticrateswerehigherthantheirmotherplants.Thesportvarietieshadsignificantdistinctflowercolorsaswellasleafcolorsfromtheirmotherplant.ThevarietieswereelitegermplasmresourceforbreedingofL.chinensevar.rubrum.EspeciallytheNo.2sportvarietycouldbeaprospectivecommercialproduct.KeywordsLoropetalumchinensevar.rubrumbudsportleafvariegationLoropetalumchinensevar.rubrumHamamelidaceae2002、、2009。1.58%Loropetalumchinense1983。214182009。2006‘’3。3。1999。。Hirokazuetal.2004、Mandaletal.2000Michael2001Wataruetal.2004、Berettaetal.1997、Lee1988。、、222007。‘’32、‘’。11.1200692‘’3。‘’1‘’2‘’3Ⅰ。‘’1‘’212CK。Glycinemax、Cucumissativus、Capsicumannuum、Solanumlycopersicum5SolanumtuberosumXpotatoXvirusPVX。1Tab.1NamesandcodesonvariegatedbudsportsandmaternalplantsofL.chinensevar.rubrumCodesNamesM‘’‘MizhiMeihong’M1‘’1Variegatedbudsportof‘MizhiMeihong’No.1M2‘’2Variegatedbudsportof‘MizhiMeihong’No.2M3‘’3Variegatedbudsportof‘MizhiMeihong’No.31.21.2.12008、PVX10g75%10s4100.01mol·L－1pH7.04℃5000g2min。、、、、、5mL、5mL1。15。1.2.219935mLⅠ1、235mL1∶1∶1ⅡPVX5mLⅢ。5min3。2%UAUO2CH3COO2·2H2OpH4.0～5.275465min3。30minMSCKAF10001。1.2.32007—2009‘’1、2、3125、250、35025cm×25cm、40cm×40cm、50cm×50cm。2008—2009、、1/15hm2‘’。1.2.43～4。12MoticSMZ-143。Motic3。1、Fig.1TheresultsofTEMviewonL.chinensevar.rubrumvariegatedbudsportsofL.chinensevar.rubrumandS.tuberosumⅠ‘’Ⅱ‘’1、2、3ⅢPVX。ⅠTheresultsofTEMviewonthecrudesapfromtissueof‘MizhiMeihong’ⅡTheresultsofTEMviewonthemixedcrudesapfromtissueofvariegatedbudsportsof‘MizhiMeihong’No.1No.2andNo.3ⅢTheresultsofTEMviewonthecrudesapfromtissueofpotatoinfectedPVXvirus.1.2.520081127a、b、、2006。1.2.612008111415900—1130Li-6400-02BPDF0～2000μmol·m－2s－115020508010020030040070080010001200150018002000μmol·m－2s－1Pn。32±1℃CO2400μmol·m－2s－180%±1%。Walker1989。220081116Li-CORLi-6400Li-6400-02BPDF400μmol·m－2s－1PnCond、Tr、CO2ci。Ls=1－ci/caWUE=Pn/Tr。caCO2400±10μmol·mol－1。3。1.2.7Excel2003SPSS13.0。22.1、、、‘’、‘’1、23、。PVX。。8581－ⅠⅡ。PVX515nm13.6nm1－Ⅲ。2.22008—2009、、‘’3。。2.32‘’3、、、。。2①Tab.2ComparisonoflaminaanatomicalcharactersbetweenvariegatedsportsandmaternalplantsofL.chinensevar.rubrumμmMaterialBTUETPTTSTTLETM58.79±5.67b7.23±1.22c28.13±4.81b20.49±1.43b5.60±1.09bM159.77±9.91c5.44±1.46a28.27±8.50b20.71±3.57b6.04±1.38cM246.13±7.31a5.63±1.11a20.37±4.50a16.59±3.87a4.31±1.14aM359.84±5.08c7.07±1.14bc29.57±2.76c20.49±3.70b8.07±1.87d①BTUETPTTSTTLET。P0.05。。BTLeafthicknessUETUpperepidermisthicknessPTTPalisadetissuethicknessSTTCavernoustissuethicknessLETLowerepidermisthickness.Valuesinacolumnbythedifferentsmalllettersaresignificantlydifferentatthe0.05level.Thesamebelow.2.42009。3‘’3a、b、a+b。33。33。3①Tab.3ComparisonoflaminapigmentcontentsbetweenvariegatedsportsandmaternalplantsofL.chinensevar.rubrummg·g－1MaterialaChlabChlba+bChla+bCarotenoidAnthocyaninM1.12±0.11b0.97±0.15c2.09±0.08b1.05±0.06c7.50±0.09dM10.84±0.13a0.75±0.14b1.59±0.03a0.75±0.05ab2.10±0.01bM20.73±0.04a0.79±0.02b1.52±0.12a0.66±0.07a1.53±0.12aM30.90±0.03a0.43±0.01a1.33±0.11a0.79±0.09b4.08±0.05c①2008210—2009331。Thetimeofobservationwasamong10thFebruary2008to31thMarch2009.2.5。4‘’3、、。‘’213.51μmol·m－2s－1‘’1369.83μmol·m－2s－1。5‘’3、、CO2、、。322。‘’1。95464①Tab.4Comparisonofphotosyntheticparametersoflight-responsecurvebetweenvariegatedbudsportsandmaternalplantsofL.chinensevar.rubrumμmol·m－2s－1MaterialPnmaxRdLCPLSPM14.35±2.01a1.84±0.45a20.58±0.34b296.58±3.12cM120.94±0.67b2.17±0.34b20.90±1.09b369.83±0.94dM214.33±0.88a2.11±0.81b13.51±0.99a283.87±1.05aM314.76±0.91a1.81±0.64a22.82±2.08c291.11±0.92b①PnmaxRdLCPLSP。PnmaxMaximumnetphotosyntheticrateRdDarkrespirationrateLCPLightcompensationpointLSPLightsaturationpoint.5①T