马尔尼菲青霉的分子生物学研究进展

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··510120【】HIV。。、。【】【】R379.9【】B【】1673-3827201003-0188-05PenicilliposismarneffeiPSMPenicilliummarneffeiPM1。PSM、、、、2。PSMAIDS、3HIV4。PM25℃37℃5。。PM、、2。PM、5。PM2。PSMPM6。PSM、PM。8151008901000073..E-maildomy2008@qq.comE-mailjunminmx@163.com1PM、。1996VanittanakomRFLPHaeIIIPSM46PMDNAⅠⅡ。PSM221672.7%Ⅰ627.3%ⅡⅠPSM7。Hsueh24PSMPM8。2001TrewatcharegonNotIPF-GE69PMMPIMPII9MPIatoMPIfMPIIatoMPIIcVanittanakom。9。2000PankornGA-CA4M13ChiangRaiBangkok30PMPCRA、B、C、DPSM10。2004LaskerRanPMMPSM。35PM22D=0.956。·881·2010653ChinJMycolJune2010Vol5No.3PM11。FisherMLMTPM。。12。MLMTPMPasqualottoPCR。PCR13。2。。。BornemanPMabaAPM1030。25℃PM。37℃。。abaAPMabaA14。Borne-manPMSTE12stlA。PMstlA、。PMstlAsteA15。BornemanPMAPSESstuA。PM。PMstuAstuA。PM16。G。ZuberPMGαgasAfadA。PMgasAgasAPM。gasA。PM37℃17。ZuberPMGαgasCαα。gasCgasCgasCPM。gasC25℃PMgasA18。BoycePMRaccflBCdc42cflA。cflBcflAG。clfB。clfB。clfAPM。clfB。PM19。BoycecflAcflBRasGra-sA。rasAclfACdc42clfAPMrasAclfA。cflACdc42cflBRacPMcf-lAcflB20。。BoycePMpakAGp21。p2137℃·981·2010653ChinJMycolJune2010Vol5No.3PM。25℃PSM。cflAp21。PM。PM21。37℃。Boycep21pakB。PMpakB37℃。PM37℃。25℃pakBPM。PM22。PongsunkxyltbpAtbpA。TbpAPM25℃37℃。TbpAPMPM23。3。LobuglioPMrDNAPMDNA24。LindsleyITS4ITS5PM、、25。VanittanakomPCRPCRPM1.0pg/μL1.8fg/μL。13、121400bp、DNADNA。2dPCR7hPCR10h4~7d26。PrariyachatigulPCRPM10pgDNA250DNA27。PCRPCRPM。Tsune-miPSMDNAPCRPSM28。Zeng14PSM10DNAPCRPCR14pg/μL14fg/μL29。PSM。PMrDNA12PM。12。PM30。HsiuerDNAITS1ITS2116PM100%16~24h31。PongpomPCRPMDNAPCR15304pg/μl0.4fg/μl。PSM352468.6%32。PornprasertTaqManPCRPM5.8SrRNADNA。10PM33。4HIVPSM。PMPSMPSM。1SegretainG.Penicilliummarneffein.sp.agentofamycosisofthereticuloendothelialsystemJ.Mycopathologia19593011327-353.2VanittanakomNCooperCRJrFisherMCetal.Penicillium·091·2010653ChinJMycolJune2010Vol5No.3marneffeiinfectionandrecentadvancesintheepidemiologyandmolecularbiologyaspectsJ.ClinMicrobiolRev200619195-110.3SupparatpinyoKKhamwanCBaosoungVetal.DisseminatedPenicilliummarneffeiinfectioninsoutheastAsiaJ.Lancet19943448915110-113.4LiPCTsuiMCMaKF.Penicilliummarneffeiindicatordis-easeforAIDSinSouthEastAsiaJ.AIDS199262240-241.5.J.200515182743-2745.6.J.200725312-314.7VanittanakomNCooperCRJrChariyalertsakSetal.Restric-tionendonucleaseanalysisofPenicilliummarneffeiJ.JClinMicrobiol19963471834-1836.8HsuehPRTengLJHungCCetal.MolecularevidenceforstraindisseminationofPenicilliummarneffeianemergingpathogeninTaiwanJ.JInfectDis200018151706-1712.9TrewatcharegonSSirisinhaSRomsaiAetal.MoleculartypingofPenicilliummarneffeiisolatesfromThailandbyNotImacrore-strictionandpulsed-fieldgelelectrophoresisJ.JClinMicrobi-ol200139124544-4548.10ImwidthayaPThipsuvanKChaiprasertAetal.PenicilliummarneffeitypesanddrugsusceptibilityJ.Mycopathologia20011493109-115.11LaskerBARanY.Analysisofpolymorphicmicrosatellitemark-ersfortypingPenicilliummarneffeiisolatesJ.JClinMicrobi-ol20044241483-1490.12FisherMCAanensenDdeHoogSetal.Multilocusmicrosatel-litetypingsystemforPenicilliummarneffeirevealsspatiallystructuredpopulationsJ.JClinMicrobiol200442115065-5069.13PasqualottoACDenningDWAndersonMJ.AcautionarytalelackofconsistencyinallelesizesbetweentwolaboratoriesforapublishedmultilocusmicrosatellitetypingsystemJ.JClinMi-crobiol2007452522-528.14BornemanARHynesMJAndrianopoulosA.TheabaAhomo-logueofPenicilliummarneffeiparticipatesintwodevelopmentalprogrammesconidiationanddimorphicgrowthJ.MolMicro-biol20003851034-1047.15BornemanARHynesMJAndrianopoulosA.AnSTE12homo-logfromtheasexualdimorphicfungusPenicilliummarneffeicomplementsthedefectinsexualdevelopmentofanAspergillusnidulanssteAmutantJ.Genetics200115731003-1014.16BornemanARHynesMJAndrianopoulosA.Abasichelix-loop-helixproteinwithsimilaritytothefungalmorphologicalregula-torsPhd1pEfg1pandStuAcontrolsconidiationbutnotdimor-phicgrowthinPenicilliummarneffeiJ.MolMicrobiol2002443621-631.17ZuberSHynesMJAndrianopoulosA.G-proteinsignalingme-diatesasexualdevelopmentat25degreesCbuthasnoeffectonyeast-likegrowthat37degreesCinthedimorphicfungusPenicilliummameffeiJ.EukaryotCell200213440-447.18ZuberSHynesMJAndrianopoulosA.TheG-proteinalpha-subunitGasCplaysamajorroleingerminationinthedimorphicfungusPenicilliummarneffeiJ.Genetics20031642487-499.19BoyceKJHynesMJAndrianopoulosA.Controlofmorphogene-sisandactinlocalizationbythePenicilliummarneffeiRAChomologJ.JCellSci200311671249-1260.20BoyceKJHynesMJAndrianopoulosA.TheRasandRhoGT-Pasesgeneticallyinteracttoco-ordinatelyregulatecellpolarityduringdevelopmentinPenicilliummarneffeiJ.MolMicrobi-ol20055551487-1501.21BoyceKJAndrianopoulosA.Ap21-activatedkinaseisrequiredforconidialgerminationinPenicilliummarneffeiJ.PLoSPathog2007311162.22BoyceKJSchreiderLAndrianopoulosA.Invivoyeastcellmor-phogenesisisregulatedbyap21-activatedkinaseinthehumanpathogenPenicilliummarneffeiJ.PLoSPathog2009511e1000678.23PongsunkSAndrianopoulosAChaiyarojSC.ConditionallethaldisruptionofTATA-bindingproteingeneinPenicilliummarneffeiJ.FungalGenetBiol20054211893-903.24LoBuglioKFTaylorJW.PhylogenyandPCRidentificationofthehumanpathogenicfungusPenicilliummarneffeiJ.JClinMicrobiol199533185-89.25LindsleyMDHurstSFIqbalNJetal.Rapididentificationofdimorph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