鸡白细胞介素_2分子生物学和疾病防治应用研究进展

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20103213ChinaPoultryVol.32,No.13.2010-2(interleukin-2,IL-2)T,。,T,,[1]。,。,,、,。IL-2,、。,IL-2。IL-2。1IL-2Sundick[2]ConAcDNA,COS-7,,IL-2。IL-2mRNA747bp,343bp,143。,IL-2IL-15,mRNA5′(untranslatedregion,UTR),mRNAT。,22,IL-2,IL-15。4,2,2,1。,3′ATTTA,,IL-2mRNA。Kaiser[3]RACEIL-2。,,,IL-2,Tap,4ANX5,,,。2IL-2IL-2,。,、、、-2,*,(,063001):2009-12-05:2010-01-06*,E-mail:wanghaoqing1230@sina.com:-2(IL-2),。,IL-2,。。:;-2;;41--20103213ChinaPoultryVol.32,No.13.2010,,IL-2,IL-2[4]。Lowenthal[5],IL-2IgA。2.1IL-2(Marek′sdisease,MD)MD,MD、、、。,MD。,IL-1βIL-2LaSota[6]。2.2IL-2(infectiousbursaldisease,IBD)IB,。IL-2,,。[7]IL-2IBD,,IL-2。[8]IL-2PCI-IL-2ZJ111(PCI-IL-2),(IBDV)DNA,,IL-2IBDVDNA,IBDVDNA,TB。[9]IL-2IBDV(VP2/VP4/VP/3)pCICMV,DNA,,IBDVDNA,。2.3IL-2(Newcastledisease,ND)(aavianinfluenza,AI)[10]IL-2NDD26F,IL-2NDF,,,IL-2NDF。IL-2ND22,IL-2HI,。[11]IL-2IL-15,AI,。2.4IL-2Lillehoj[12]IL-2,。,IL-2,,。[13]TA4、IL-2、TA4-IL-2,17、24,、、。,,;:2~4,;:、。,,。2.5IL-2IL-2TIL-1[14]。,T,IL-2T。[15]Qwin,A(SIgA),IL-2SIgA,,IL-2SIgA,。,SPF,、,、,,,TIL-2[16]。3,42--20103213ChinaPoultryVol.32,No.13.2010,、、、,。IL-2、。,,。,,。,IL-2,,。:1KogutM,RothWL,KaiserP.Differentialeffectsofageonchickenheterophilfunctionalactivationbyrecombinantchickeninterleukin-2[J].DevCompImmunol,2002,26(9):817-830.2SundickRS,Gill-DixonC.Aclonedchickenlym-phokinehomologoustobothmammalianIL-2andIL-15[J].JImmunol,1997,159(2):720-725.3KaisserP,MarianiP.Promotersequence,exon:intronstructure,andsyntenyofgeneticlocationshowthatachickencytokinewithT-cellproliferationactivityisIL-2andnotIL-15[J].Immunogenetics,1999,49:264RavasAL,FabricantJ.Indicationsofimmunodepres-sioninchickeninfectedwithvariousstainsofmarekdiseasevirus[J].AvianDisease,1988,32:1-8.5LowenthalTW,ConnickTE,ObranovichTD,etal.DevelopmentofTcellimmuneresponsivenesinyoungchickens[J].AvianImmunologyinProgress,1993,113-1186,,,.IL-1βIL-2cMGFLaSota[J].,2006,36(8):617-620.7,,.IL-2[J].,2005,41(6):16-17.8,,,.2DNA[J].(),2007,33(3):237-242.9,,,.2DNA[J].,2001,17(6):652-657.10,,,.FIL-2[J].,2001,23(2):81-83.11,,,.IL-2IL-15[J].,2006,36(10):805-810.12LillehojHS,KangSY,Ke11erL,etal.EimeriatenellaandE.acervulina:LymphokinesseceretedbyanavianTcelllymphomaorbyaporozoit-stulatedimmuneTlmphocytesprotectchickensagainstaviancoccidiosis[J].ExpParasitol,1987,69:54-6413,,,.-2[J].,2005,33(2):290-293.14,,.2(IL-2)[J].,2005,27(19):17-20.15,,.2[J].,2007,12:112-116.16,.SPF[J].,1999,20(3):50-54.“”,3,60%、80%“”。“”、、,,。“”,,,“”。,“”60%~90%、10%~15%、50%~70%,。,100~150,1.0~1.5,、。“”,3,、300。●●43--

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