1分子生物学BYKAI

MolecularBiology《分子生物学》主讲：开国银教授姓名：开国银出生年月：1977年3月最高学历：理学博士技术职务：教授毕业学校：上海交通大学专业：生物化学与分子生物学研究方向：植物基因工程个人简介本人的科研背景近期发表植物基因工程领域文章100多篇，其中SCI收录60多篇。在国际基因库登录新基因30多条，并申请中国发明专利24项！主持国家及省部级科研项目10多项。SCI论文发表情况1.GuoyinKai,ZhiqiMiao,LeiZhang,DongliZhao,ZhihuaLiao,XiaofenSun,LingxiaZhao,KexuanTang.Molecularcloningandexpressionanalysesofanewgeneencoding3-hydroxy-3-methylglutaryl-CoAsynthasefromTaxusxmedia.BiologiaPlantarum2006,50(3):359-3662.GuoyinKai,JihongJiang,DongliZhao,LingxiaZhao,LeiZhang,ZhugangLi,BinhuiGuo,XiaofenSun,ZhiqiMiao,KexuanTang.IsolationandexpressionprofileanalysisofanewcDNAencoding5-alpha-taxadienol-10-beta-hydroxylasefromTaxusmedia.JournalofPlantBiochemistryandBiotechnology2006,15(1):1-53.GuoyinKai,LingxiaZhao,LeiZhang,ZhugangLi,BinhuiGuo,DongliZhao,XiaofenSun,ZhiqiMiao,KexuanTang.CharacterizationandexpressionprofileanalysisofanewcDNAencodingtaxadienesynthasefromTaxusmedia.JournalofBiochemistryandMolecularBiology2005,38(6):675-6854.GuoyinKai,ZhiqiMiao,ChengxiangQiu,LeiZhang,LingxiaZhao,ZhugangLi,TiefengXu,LidaZhang,YifuGong,DongliZhao,DonghuiLiu,XiaofenSun,KexuanTang.MolecularcloningandcharacterizationofataxadienolacetyltransferasecDNAfromTaxusmedia.Plantscience,2004,167(4):759-7645.GuoyinKai,LingxiaZhao,JinguiZheng,LeiZhang,ZhiqiMiao,XiaofenSun,KexuanTang.Isolationandcharacterizationofanewmannose-bindinglectingenefromTaxusmedia.JournalofBiosciences,2004,29(4):399-4076.GuoyinKai,JinguiZheng,LeiZhang,YongzhengPang,ZhihuaLiao,ZhugangLi,LingxiaZhao,XiaofenSun,KexuanTang.MolecularCloningofaNewLectinGenefromZ.grandiflora.DNASequence,2003,14(4):335-3387.GuoyinKai,LeiZhang,HongyuZhang,TiefengXu,KexuanTang,HanmingZhang.Markerfree:anoveltendencyoftransgenicplants.ActaBotanicaSinica.2002,44(8):883–8888.JihongJiang,GuoyinKai,XiaoyinCao,FengmeiChen,DongningHe,QunLiu.MolecularcloningandfunctionidentificationofanewHMG-CoAreductasegenefromEucommiaulmoidesOlive.Biosciencereports2006(inpress)9.LeiZhang,GuoyinKai,BeibeiLu,HanmingZhang,KexuanTang,JihongJiang,WanshengChen.MetabolicEngineeringofTropaneAlkaloidBiosynthesisinPlants.JournalofIntegrativePlantBiology2005,47(2):136-1410.ZhonghaiChen,GuoyinKai,XiaojunLiu,JuanLin,XiaofenSun,KexuanTang.Molecularcloningandcharacterizationofamannose-bindinglectingenefromZingiberofficinaleRoscoe(ginger)rhizomesJournalofBiosciences2005,30(2):213-22011.LeiZhang,GuoyinKai,TiefengXu,YanPi,HanmingZhang,XiaofenSun,KexuanTang.EfficientregenerationoftetraploidIsatisindigoticaplantsviaadventitiousorganogenesisfromhypocotylexplants.BiologiaPlantarum,2004,48(1):121-12412.ZhihuaLiao,YifuGong,GuoyinKai,KaijinZuo,MinChen,QiuminTan,YaminWei,LiangGuo,FengTan,XiaofenSun,KexuanTang.AnIntron-freeMethylJasmonateInducibleGeranylgeranylDiphosphateSynthaseGenefromTaxusmediaanditsfunctionalidentificationinyeast.MolecularBiology.2005，39(1):14-20.13.ZhugangLi,LingxiaZhao,GuoyinKai,ShunwuYu,YoufangCao,YongzhenPang,XiaofenSun,KexuanTang.Cloningandexpressionanalysisofawaterstress-inducedgenefromBrassicaoleracea.Plantphysiologyandbiochemistry.2004,42(10):789-79414.ZhangLei,HuangBeiBei,KaiGuoYin,GuoMeiLi.Analysisofinter-populationgeneticvariationinChineseCarthamustinctoriusL.usingAFLPmarkers.ActaPharmaceuticaSinica2006,41(1):91-9615.ZhugangLi,ChongshiCui,LingxiaZhao,GuoyinKai,LidaZhang,XiaofenSun,KexuanTang.Molecularcloningandcharacterizationofananti-boltingrelatedgene(BcpFLC3)fromBrassicacampestrisssp.Pekinensis.PlantScience2005,168:407-41316.LingxiaZhao,ChengxiangQiu,JingfuLi,YourongChai,GuoyinKai,ZhugangLi,XiaofenSun,KexuanTang.InvestigationofdiseaseresistanceandcoldtoleranceofSolanumlycopersicoidesfortomatoimprovement.HortScience2005,40(1):43-4617.YifuGong,ZhihuaLiao,MinChen,KaijinZuo,LiangGuo,QiuminTan,ZhushiHuang,GuoyinKai,XiaofenSun,FengTan,KexuanTang.Molecularcloningandcharacterizationofa1-deoxy-D-xylulose5-phosphatereductoisomerasegenefromGinkgobiloba.DNASequence2005,16(2):111-12018.LeiZhang,RuxianDing,YourongChai,MercedesBonfill,M.TeresaPiñol,TiefengXu,YanPi,ZinanWang,HanmingZhang,GuoyinKai,ZhihuaLiao,XiaofenSun,KexuanTang.EngineeringtropanebiosyntheticpathwayinHyoscyamusnigerhairyrootcultures.ProcNatlAcadSciUSA，2004,101(17):6786-6791.1.开国银、苗志奇、唐克轩.红豆杉3-羟基-3-甲基戊二酰辅酶A合成酶蛋白编码序列。中国专利发明申请号:200410024891.0；公开号CN1584033A2.唐克轩、开国银、苗志奇、李柱刚、赵凌侠。红豆杉烷C13-侧链－N－苯甲酰转运酶蛋白编码序列。中国专利发明申请号:200410016769.9；公开号CN1560255A3.唐克轩、开国银、苗志奇、李柱刚、赵凌侠。红豆杉烷凝集素蛋白编码序列。中国专利发明申请号:200410016709.7；公开号CN1560250A4.唐克轩、开国银、孙小芬、庞永珍、姚剑虹、郑金贵。一种韭莲凝集素蛋白及其编码序列。中国专利发明申请号：02111817.5；公开号CN1459503A5.唐克轩,李柱刚，赵凌侠，孙小芬，开国银。大白菜BcpFLC3蛋白编码序列。中国专利申请号：03116982.1；公开号CN1472320A（授权专利号为ZL03116982.1）6.蒋继宏、曹小迎、开国银、陈风美。杜仲3-羟基-3-甲基戊二酰辅酶A还原酶蛋白编码序列。中国专利发明申请号:200410065000.67.蒋继宏、曹小迎、开国银、陈凤美、卢芳。南京椴3-羟基-3-甲基戊二酰辅酶A还原酶蛋白编码序列。中国专利申请号：200410016709.7专利申请1.上海市自然科学基金项目（05ZR14093):利用基因工程技术提高三分三中东莨菪碱含量的研究（2005.9-2007.12）主持执行顺利2.上海市青年科技启明星计划（06QA14038）:喜树碱生物合成的代谢工程研究（2006.6-2008.12）主持执行顺利3.上海市教委面上项目：利用毛状根转化技术获得三分三莨菪生物碱的研究（2006.6-2008.12）主持执行顺利4.上海市科委西部开发科技合作项目（065458022）：云南省石斛种质资源的收集保护与开发利用（2006.8-2008.12）主持执行顺利5.国家“863”项目（NO.2002AA212191)：利用基因工程技术提高红豆杉中紫杉醇及其前体含量的研究（2002.12-2005