20112分子生物学试题A

1卷号：（A）（2011年12月）绝密湖北师范学院普通本科期末考试试卷分子生物学考试范围全部章节命题人王友如系部生命科学学院考试形式闭卷课程类别必修学期20112专业生物技术大题号一二三四五六七八九十班级题分162410202010学号得分姓名阅卷人总分———————————————————————————————————一、名词解释（翻译并简单解释，每小题2分，共16分）1.tRNAi2.RNAi3.intein4.intron5.codingregion;6.codingstrand;7.operon8.operator;二、单项选择题(选择正确答案，每小题1.5分，共24分)9.WhichenzymedoesnotplayaroleinDNAreplication?()(A)DNApolymerase(B)Helicase(C)Single-strandedbindingprotein(D)Guanyltransferase10.Splicingistheprocessthatdoeswhichofthefollowing?()(A)Removeintronsandconserveexons(B)Removeexonsandconserveintrons(C)RemovemutatedregionsofprimarytranscriptRNA(D)AddmultipleadenosinebasestotheendofaprimaryRNAtranscript11.The5'cappingprocesscreateswhattypeoflinkage?()(A)5'-3'(B)3'-5'(C)5'-5'(D)3'-3'12.Codonsthatcodeforthesameaminoacidarecalled:()(A)Synonyms(B)Similar(C)Degenerates(D)Complements本题得分本题得分213.Whichvectorwouldyouchoosetoexpressaforeigngeneinaplant?DAabaculovirusvector.Baretroviralvector.CaYepvector.DaT-DNAvector.14.Whatsignalstheendoftranscription?()(A)Stopcodon(B)Terminator(C)TheendoftheDNAchain(D)RNApolymeraserunsout15.Whatisthenameofthemoleculethatisaddedtothe5'endofeukaryoticRNAtranscripts?()(A)ATP(B)SnRNP(C)GTP(D)Phosphate16.Accordingtothewobblehypothesis,aUinthe5'positionoftheanitcodoncanpairwith:()(A)UorC(B)AorG(C)A,C,orG(D)OnlyA17.Whichtwoofthefollowingstatementsabouttranscriptionfactorsaretrue?DAthehelix-turn-helixdomainisatranscriptionalactivationdomain.Bdimerizationoftranscriptionfactorsoccursthroughthebasicdomain.CleucinezippersbindtoDNA.DitisoftenpossibletogetfunctionaltranscriptionfactorswhenDNAbindingdomainsandactivationdomainsfromseparatetranscriptionfactorsarefusedtogether.18.Whichofthefollowingisnotcharacteristicofprokaryoticterminators?()(A)Rho-independentterminators(B)Rho-dependentterminators(C)TheTATAbox(D)Hairpinloops19.WhatistheenzymeresponsibleforstitchingtogetherOkazakifragments?()(A)Helicase(B)Single-strandedbindingprotein(C)Primase(D)Ligase20.DenaturationofdoublestrandedDNAinvolves.Apreakageintoshortdouble-strandedfragments.Bseparationintosinglestrands.ChydrolysisoftheDNAbackbone.Dcleavageofthebasesfromthesugar-phosphatebackbone.21.Inprokaryotes,thelaggingstrandprimersareremovedby...A3'to5'exonuclease.BDNAligase.CDNApolymeraseI.DDNApolymeraseIII.22.Prokaryoticplasmidscanreplicateinyeastcellsiftheycontainaclonedyeast..AORC.BCDK.CARS.DRNA.23.Pernucleotideincorporated,thespontaneousmutationfrequencyinE.coliis...A1in106.B1in108.3C1in109.D1in1010.24.Whichtwoofthefollowingstatementsaboutattenuationatthetrpoperonaretrue?Aattenuationisrho-dependent.Bdeletionoftheattenuatorsequenceresultsinanincreaseinbothbasalandactivatedlevelsoftranscriptionfromthetrppromoter.Ctheattenuatorliesupstreamofthetrpoperatorsequence.Dattenuationdoesnotrequiretightcouplingbetweentranscriptionandtranslation.Epausingofaribosomeattwotryptophancodonsintheleaderpeptidewhentryptophanisinshortsupplycausesattenuation.25.Whichoneofthefollowingstatementsaboutcatabolite-regulatedoperonsisfalse?AcAMPreceptorprotein(CRP)andcataboliteactivatorprotein(CAP)aredifferentnamesforthesameprotein.BwhenglucoseispresentinthecellcAMPlevelsfall.CCRPbindstocAMPandasaresultactivatestranscription.DCRPbindstoDNAintheabsenceofcAMP.ECRPcanbendDNA,resultinginactivationoftranscription.三、填空题(每空1分，共10分)26.Thesimplestcloningvectoratleastcontains,andorielements.27.ThediagrambelowshowstwoRNAmolecules.MoleculeIisprocessedtogivemoleculeII.Answerthefollowingquestionsaboutthesetwomolecules:Wheredoesthisconversionhappen?____________________________WhattypeofRNAmoleculeismoleculeI?_________________________WhattypeofRNAmoleculeismoleculeII?_________________________Nametheregions/structuresindicatedbythearrows:A=_________________B=_______________C=_________________D=______________E=________本题得分4四、简答题（回答要点,每小题4分，共五小题，共20分）28WhenyoustudytheDNAcomponentsofphageΦΧ174,yougettheresultsasfollows:A，25%；T，33%；G，24%；C，18%.Question(1):Doesyourresultsabidebybasepairingrules?Question(2):Howdoyouexplainyourresults?29.DNAligaseplaysimportantroleintheprocessofDNAreplication,butligasedoesnotfunctionintheprocessofRNAsynthesis.Couldyouexplainit?30DoyouthinkthatrRNAandtRNAaremorestablethanmRNA?Why?31.HowdoesacodingregionDNAsequencecodedifferenttypesofprotein?32.Couldyoulist4kindsofRNAthathavetheabilitytocatalyticactivity?五、问答题（每小题10分，共20分）。33.PleasetellusthetypesofRNAthatyouknow,itwouldbebetterthatyouexplaintheirfunctioninlivingorganisms.(10points)34.Takethecaseofarabinoseoperon,Couldyouexplainwhatcis-actingelementandtrans-actingfactorare,howdotheyinteractwitheachothertoregulatethisoperon?(10points)六、分析题（每小题10分，共10分）35.AscientistgotagalpromotermutantE.coli.whichcannotusegalactose(半乳糖)inthemedium,ifhemutatedthegenecya(geneofcAMP环化酶)andcrp(cAMPAcceptorProteingene)weremutatedintocya-andcrp-respectively,thesecondmutatedE.coli.restoreditsabilitytousegalactose.Couldyouexplaintheresultsbyusingyourmolecularbiologicalknowledge?本题得分本题得分本题得分