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doi:103969/jissn1003-6245201112011唐保晖:(HIV)HIV,HIV,HIV,HIV,(ELISA)HIV,WB,HIVHIV,HIV,:;HIV;:R51291:A:1003-6245(2011)12-1108-03:,547000(HIV),,HIV,HIV,HIV,HIV,,HIV,HIV1HIVHIV()[1]11筛查试验111(ELISA),HIVELISA,,,,(),ELISA,ELISAELISAP24HIV-1/2HIV-1/2,4~9d,[2]112,,HIV,HIV,,113(RT),10~30min1131(PA)HIVHIV,HIV,,PA2:HIV-1HIV-2HIV-1HIV-21132ELISA(),,HIV,10min1133,HIV,(),HIV-1MO[3][4],12HIV抗体确证方法(WB)(RIPA)(IFA)121(WB)HIV,(2009),,HIV,,,[2],,,,,WBHIV,,,2%[5]4%~20%HIV,WBP24[6]122,,11082011122712JMedPestControl,Dec2011,Vol27No12123(IFA)HIV,,,,IFA,FDAWB,,,124(RIPA)HIV,,,,,IgGA(),HIV,HIVSDS-PAGE,HIV-11601206651~5541312417kDHIV,,,2HIVHIV-1HIVP24HIV,P24RNA,,,,P24,HIV,HIVP24ELISA,,HIV-1P24HIV,,HIV,,50%~80%ELISAP24HIV-1,P24P24(immunecomplexdisassociate,ICD),ELISA(signalamplificationboostedELISA),(linealimmunoenzymaticassay,LIA)(ultrasensitiveenzymeimmunoassay,UEI),(immunosorbentelectronmicroscopicalmethod,ISEM)(nanoparticlebasedbiobarcodeamplificationassay,BCA),ICD90%[7],LIA001pg/mL[8],3HIVHIV2,PCRRT-PCR,HIVgag/pol/env/LTRRNA,HIV,HIV,HIV,HIV;HIV,,HIVHIV,HIVHIVRNAPCR(RT-PCR)(NASBA)DNA(bDNA)PCR,,PCRHIV,,,,,,,4HIVHIV2HIV,;HIV,(IC50IC90),,HIV,PCR(RT-PCR)HIVHIV5CD4+CD8+TCD4+CD8+T2,,CD4+THIV,;;HIV;;CD8+T/T,HIVCD8+T,CD4/CD8,,CD4T/CD8T2,1,[9]6HIV(HIV,PBMC)(PBMC),PBMC,,HIVHIV,HIVHIVHIVHIV;HIVHIV;HIV7HIV-1HIV,,HIV(下转1111页)11092011122712JMedPestControl,Dec2011,Vol27No123,///(GAVI)33,2004,-HBs1~6,-HBs7667%,-HBs4857%,(85%),:,,,,,,,,,,,,,,,,,,,,[1][S]2005[2],,,[J],2006,22(2):88-90[3][J],1998,6(2):67-69(:2011-06-17)(上接1109页),BED-EIAHIV-1EIA(BED-CEIA),HIVIgGIgGBED-CEIAHIV,,BED,BED,,,FDABED-CEIA,,,,,,,,,,,,,,,[1][S]2009[2]-[M]2009:361-376[3]BernardMBranson,MDRapidTestforHIVAntiboby[J]AIDSReviews,2000,2:76-83[4]HIV1+2[J],2003,9(5):280-281[5]NguyenKA,BuschMPEvolvingstrategiesfordiagnosinghumanimmunodeficiencyvirusinfection[J]AmJMed,2000,109(7):595-597[6]CelumCL,CoombsRW,JonesM,etalRiskfactorsforrepeatedlyreactiveHIV-1EIAandindeterminatewesternblotsApopulationbasedcase-controlstudy[J]ArchInternMed,1994,154(10):1129-1137[7],,HIVP24[J]:,2003,10(4):111-114[8],HIV[J]:,2003,10(3):65-69[9],,,HIV/AIDSCD4+CD8+T[J],2004,10(2):83-85(:2011-05-17)11112011122712JMedPestControl,Dec2011,Vol27No12