橡胶树棒孢霉落叶病病原菌的生物学特性

2010,36(2):98-101PlantProtection:2009-03-24:2009-04-17:(2008XJ01)*Tel:[phone];Email:[e-mail]张春霞*,何明霞,李加智,王进强,蒋桂芝,侯建勇(,666100)通过对橡胶多主棒孢进行生物学特性研究,结果表明:菌丝生长的适宜温度范围为25~30,最适温度为30;最适pH范围为4~8;菌丝在以麦芽糖为碳源和以KNO3为氮源的培养基上生长最好分生孢子萌发的适宜温度范围为20~35,最适温度为25;水滴是孢子萌发的必要条件;在不同的pH条件下分生孢子萌发无明显差异;分生孢子在果糖中萌发率最低橡胶树;多主棒孢;生物学特性:S435.76:ADOI:10.3969/j.issn.0529-1542.2010.02.021BiologicalcharacteristicsofCorynesporacassiicolacausingCorynesporaleaffalldiseaseZhangChunxia,HeMingxia,LiJiazhi,WangJinqiang,JiangGuizhi,HouJianyong(YunnanTropicalCropsResearchInstitute,Jinghong666100,China)AbstractThebiologicalcharacteristicsofCorynesporacassiicolawasreportedinthispaper.Theresultsshowedthattheoptimumtemperatureformycelialgrowthis25-30,but30isthemostsuitabletemperature.TheoptimumpHis4-8.ThemyceliumofCorynesporacassiicolagrewwellonthemediawithmaltoseasthecarbonsources.KNO3isthebestnitrogensource.Theoptimumtemperatureforconidialgerminationis20-35,but25isthemostsuitabletemperature.Waterdropisnecessaryforconidialgermination.ConidialgerminationhadnoobviouseffectsbetweendifferentpHmedia.Conidiagerminatedatalowerrateonthemediausingfructoseasthecarbonsources.Keywordsrubbertree;Corynesporacassiicola;biologicalcharacteristics[Corynesporacassiicola(Berk.&Curt.)Wei],,,[1]2007,[2],,20076,,,,34.07hm2,,,200711,57.73hm2,43.13hm2,1.6hm2,13hm2,94%[3-4],,11.1,,[5],[6],362:4mm,,,25,3105/mL,25,,,,1.21.2.1不同温度对菌丝生长的影响4mmCzapek(),51015202528303538406d,31.2.2不同pH对菌丝生长的影响Czapek,55,0.1mol/LHCL0.1mol/LNaOHpH,pH3~106d,31.2.3不同光照对菌丝生长的影响4mmCzapek,,12h3,6d,31.2.4不同碳源对菌丝生长的影响Czapek,,,,1.2.5不同氮源对菌丝生长的影响Czapek,KNO3NaNO3,,,1.31.3.1温度对分生孢子萌发的影响Czapek,20~30,,510152025303540,7h31.3.2pH对分生孢子萌发的影响0.1mol/LHCL0.1mol/LNaOHpH3~10,,,307h31.3.3碳源对分生孢子萌发的影响2%2%2%2%2%2%,,7h1.3.4湿度对分生孢子萌发的影响,,(10%25%50%75%90%),257h22.14d,!∀!∀,,2.22.2.1温度对橡胶多主棒孢菌丝生长的影响1,10~38,25~30,30,1038,54012.2.2pH对橡胶多主棒孢菌丝生长的影响2,,pH3~10pH4~8pH3,pH8,#99#20102pH2.2.3光照对橡胶多主棒孢菌丝生长的影响3,,,,46.93mm32.2.4碳源对橡胶多主棒孢菌丝生长的影响4,,,,,,,3,42.2.5氮源对橡胶多主棒孢菌丝生长的影响5,KNO3NaNO3,KNO3,252.32.3.1温度对分生孢子萌发的影响6,10~40,20~35,252035,562.3.2pH对分生孢子萌发的影响7,pH3~10,pHpHpH4,92.7%7pH#100#362:2.3.3碳源对分生孢子萌发的影响8,,,19.3%,82.3.4湿度对分生孢子萌发的影响7h,10%25%50%75%90%,3,10~38,30,pH4~8,[Corynesporacassiicola(Berk.&Curt.)Wei][6],,,;KNO3,25,pH474%,,,10~38,[1],,,.[J].,2007,21(6):372-373.[2]PuJinji,ZhangXin,QiYangxian,etal.FirstrecordofCorynesporaleaffalldiseaseofHevearubbertreeinChina[J].AustralasianPlantDiseaseNotes,2007,2:35-36.[3],,,.[J].,2008(1):54-55.[4],,.[J].,2007(6):26-30.[5].[M].3.:,1998.[6],,,.[J].,2007,28(4):73-77.[7],,,.[J].,2003(4):17-18.(97)[1],,,.[J].,2005(1):29.[2]珽,李林,李长松,等.番茄茎腐病病原鉴定及防治研究[J].,2004,34(3):286-288.[3],,.[J].,2007(5):48-49.[4].[M].:,2002:60-363.[5],.[M].:,2002:206-210.[6],,,.[J].,2005,21(2):262-264.[7].[M].:,1998.[8],,,.[J].,2002,7,23(3):33-35.[9],,,.[J].,2006,25(2):128-131.[10],,,.[J].,1999(1):37-39.[11].:[M].:,1996:438.#101#