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油溶性維他命C衍生物NIKKOLVC-IPasanActiveIngredientforAnti-AgingandWhiteningCosmeticsNIKKOCHEMICALSCO,LTD功祥貿易有限公司維他命C的功效促進膠原蛋白的合成抑制黑色素細胞抗氧化抗老化作用的效果非常不安定其他維他命C的衍生物水溶性衍生物油溶性衍生物磷酸鎂鹽維他命C(VitaminCMagnesiumPhosphate)葡萄糖苷維他命C(VitaminCGlucoside)2-棕櫚酸維他命C(VitaminCDipalmitate)Penetration&VitaminCenrichingareimportant!液態且油溶性維他命C衍生物對光和熱很安定因為是油溶性所以經皮吸收效果很好在皮膚中轉變為維他命C，呈現出維他命C的活性易溶於油中，有利於化妝品的使用C15H31-COOOCO-C15H31OCO-C15H31CHCH2OCO-C15H31OOVC-IP：VitaminCtetra-isopalmitateAscorbyltetrahexyldecanoate經皮吸收在皮膚細胞中轉變為維他命C抗氧化‧防止皮脂過氧化‧防止DNA被破壞‧防止細胞被紫外線破壞代謝活性*活化細胞*加速膠原蛋白的合成美白抗老化美白效果*抑制酪胺酸酶的活性*抑制黑色素細胞維他命C的活性VC-IPROSUVStresssignalrelease（IL、TNF-alpha）保護活性(皺紋)破壞免疫力老化發炎癌症細胞死亡防曬抗氧化消炎作用VC-IP提高免疫力COMETAssayIdeaATTTTTAAAACCGGStrongalkaliTTTAAGGAAACTCTWhatisCometAssay?Thecometassay,alsocalledthe'SingleCellGelAssay',isthetechniquetodetectDNAdamageandrepairatthelevelofsinglecells.ThecometassayorsinglecellgelelectrophoresisassayisbasedonthealkalinelysisoflabileDNAatsitesofdamage.'CometAssay'isoneofthemostpopulartestsofDNAdamagedetection(e.g.,single-anddouble-strandbreaks,oxidative-inducedbasedamage,andDNA-DNA/DNA-proteincrosslinking)byelectrophoresis,developedinrecentyears.MeritsOfCometAssay:•VeryhighsensitivitytodetectDNAdamage•Rapidandeasytohandle•Littleamountofcellsamplesneeded•Appliedtomosteukaryoticcells2-chainDNAdamagedbyUVirradiationDNAlosesonechainanddisintegratesUV1%oflowmeltingagaroseSlideglassElectrophoresismethodthatseparatesmacromolecules-eithernucleicacidsorproteins-onthebasisofsize,electriccharge,andotherphysicalpropertiesStainingofDNADNAMicroscopeobservationEpidermiscells(withVC-IP)Lysis(decompositionofcells)NeutralizationTestmethodtail(brokenDNA)headtailcellnucleuswithintactDNAControlUVB100mJ/cm2+VC-IP(100mM)UVB100mJ/cm2防止DNA被UVB破壞DNAdamagewasevaluatedbythecometassay.HaCaTkeratinocyteswhichweretreatedwithVCderivativesfor24h,wereexposedtoUVBat100mJ/cm2.Cellsarestainedwithetidiumbromide.406080100UV-blankUV+blankVC-IPVCVC-PMgVC‐GluDNAmigration(mm)BlankBlankVC-IPVCVC-PMgVC-GluUVB100mJ/cm2*****防止DNA被UVB破壞DNAdamagewasevaluatedbythecometassay.HaCaTkeratinocyteswhichweretreatedwithVCderivetivesfor24h,wereexposedtoUVBat100mJ/cm2.60.070.080.090.0100.0ControlVCNaVCPMgVCGluVCIPViability(%)**保護細胞不被UVB破壞HaCaTkeratinocytesweretreatedwithvarious100mMofvariousVitaminCderivativesfor24h.After24hfromUVBirradiation,cellsurvivalwasestimated.Significance:*p0.05,**p0.01.VC-IP防止DNA被UVA破壞VC-IP(-)VC-IP(+)00.5136（hr）8-OHdG:8-HydroxyguanosineDNAinjuryinducedbyactiveoxygenspeciesHaCaTcellsweretreatedwith80mMVC-IP.AfterUVAirradiation,8-OHdGwasdetectedimmunohistochemicallyusinganti-8-OHdGantibody.10019.851.3VC-IP(-)UVAirradiatedVC-IP(+)Control保護細胞不被UVA破壞Cellsurvival(%)HaCaTkeratinocytesweretreatedwith80mMofVC-IPfor24h.After24hfromUVAirradiation,cellswereobservedwithelectronmicroscopy.美白效果*抑制酪胺酸酶的活性*抑制黑色素細胞抗氧化*防止皮脂過氧化*防止DNA被破壞*防止細胞被紫外線破壞代謝活性*活化細胞*加速膠原蛋白的合成結論VC-IP美白抗老化1.程序:應用在凝膠的VC-IP含量為10%2.來源:10位女性(平均年齡在16-45)3.治療:一天2次治療2~10個月對於粉刺的臨床研究10%VC-IPGelBeforeuse3monthlaterBeforeuse3monthlater10%VC-IPGelBeforeuse10%VC-IPGelAfteruseVC-IP對於治療粉刺的效果10%VC-IPGelGoodExcellentNoChange防止黑色素形成促進膠原蛋白合成防止DNA被破壞skinVC-IP結論美白抗老化抗粉刺滋養NIKKOLVC-IP