SDS-PAGE凝胶配制试剂盒

SDS-PAGE凝胶配制试剂盒产品编号产品名称包装P0012ASDS-PAGE凝胶配制试剂盒可制30-50块胶产品简介：碧云天生产的SDS-PAGE凝胶配制试剂盒提供了配制SDS-PAGE凝胶所需的各种试剂，用户只需自备制胶器具和蒸馏水，即可配制PAGE胶(即聚丙烯酰胺凝胶)。SDS-PAGE凝胶配制试剂盒不仅可用于配制SDS-PAGE凝胶，也可用于配制非变性(native)PAGE凝胶。本试剂盒约可配制30-50块常规大小的PAGE胶。包装清单：产品编号产品名称包装P0012A-130%Acr-Bis(29:1)100mlP0012A-21MTris-HCl,pH8.8100mlP0012A-310%SDS5mlP0012A-4Ammoniumpersulfate(过硫酸铵)0.5gP0012A-5TEMED0.5mlP0012A-61MTris-HCl,pH6.815ml—说明书1份保存条件：1MTris-HCl,pH8.8、10%SDS、Ammonniumpersulfate(过硫酸铵)和1MTris-HCl,pH6.8室温保存。30%Acr-Bis(29:1)和TEMED4℃避光保存。过硫酸铵配制成10%溶液后，分装成小管-20℃保存，通常半年内有效。注意事项：过硫酸铵配制成10%溶液后，应当-20℃保存。同时应尽量减少室温存放时间，以防失效。TEMED易挥发，使用后请盖紧瓶盖。另外凝胶凝聚的速度和温度及光照关系密切，可通过适当调节TEMED的用量，控制在不同的室内环境下凝胶凝聚的速度。为了您的安全和健康，请穿实验服并戴一次性手套操作。使用说明：1.根据目的蛋白的分子量大小选择合适的凝胶浓度，再按照下面的表格配制SDS-PAGE的分离胶(即下层胶)：不同浓度的SDS-PAGE分离胶的最佳分离范围：SDS-PAGE分离胶浓度最佳分离范围6%胶50-150kD8%胶30-90kD10%胶20-80kD12%胶12-60kD15%胶10-40kD成分配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)6%胶51015203050蒸馏水2.04.06.08.012.020.030%Acr-Bis(29:1)1.02.03.04.06.010.01MTris,pH8.81.93.85.77.611.419.010%SDS0.050.10.150.20.30.510%过硫酸铵0.050.10.150.20.30.5TEMED0.0040.0080.0120.0160.0240.04成分配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)8%胶51015203050蒸馏水1.73.356.710.016.730%Acr-Bis(29:1)1.32.74.05.38.013.31MTris,pH8.81.93.85.77.611.419.010%SDS0.050.10.150.20.30.510%过硫酸铵0.050.10.150.20.30.5TEMED0.0030.0060.0090.0120.0180.03成分配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)10%胶51015203050蒸馏水1.32.74.05.38.013.330%Acr-Bis(29:1)1.73.35.06.710.016.71MTris,pH8.81.93.85.77.611.419.010%SDS0.050.10.150.20.30.510%过硫酸铵0.050.10.150.20.30.5TEMED0.0020.0040.0060.0080.0120.02成分配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)12%胶51015203050蒸馏水1.02.03.04.06.010.030%Acr-Bis(29:1)2.04.06.08.012.020.01MTris,pH8.81.93.85.77.611.419.010%SDS0.050.10.150.20.30.510%过硫酸铵0.050.10.150.20.30.5TEMED0.0020.0040.0060.0080.0120.02成分配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)15%胶51015203050蒸馏水0.51.01.52.03.05.030%Acr-Bis(29:1)2.55.07.510.015.025.01MTris,pH8.81.93.85.77.611.419.010%SDS0.050.10.150.20.30.510%过硫酸铵0.050.10.150.20.30.5TEMED0.0020.0040.0060.0080.0120.02注：如果配制非变性胶，参考上述配方，不加10%SDS即可配制成非变性PAGE胶。2.按照如下表格配制SDS-PAGE的浓缩胶(也称堆积胶、积层胶或上层胶)：成分配制不同体积SDS-PAGE浓缩胶所需各成分的体积(毫升)5%胶2346810蒸馏水1.42.12.74.15.56.830%Acr-Bis(29:1)0.330.50.671.01.31.71MTris,pH6.80.250.380.50.751.01.2510%SDS0.020.030.040.060.080.110%过硫酸铵0.020.030.040.060.080.1TEMED0.0020.0030.0040.0060.0080.01使用本产品的文献：1.DengXQ,ChenLL,LiNX.TheexpressionofSIRT1innonalcoholicfattyliverdiseaseinducedbyhigh-fatdietinrats.LiverInt.2007Jun;27(5):708-15.2.WangPH,GuZH,HuangXD,LiuBD,DengXX,AiHS,WangJ,YinZX,WengSP,YuXQ,HeJG.Animmunedeficiencyhomologfromthewhiteshrimp,Litopenaeusvannamei,activatesantimicrobialpeptidegenes.MolImmunol.2009May;46(8-9):1897-904.3.LiangQL,WangBR,LiGH.DcR3andsurvivinarehighlyexpressedincolorectalcarcinomaandcloselycorrelatedtoitsclinicopathologicparameters.JZhejiangUnivSciB.2009;10(9):675-82.4.DengXQ,ChengJL,ZhangYP,LiNX,ChenLL.EffectsofcaloricrestrictiononSIRT1expressionandapoptosisofisletbetacellsintype2diabeticrats.SpringerVerlag.2009.5.CaoX,ZhangY,ZouL,XiaoH,ChuY,ChuX.Persistentoxygen-glucosedeprivationinducesastrocyticdeaththroughtwodifferentpathwaysandcalpain-mediatedproteolysisofcytoskeletalproteinsduringastrocyticoncosis.NeurosciLett.2010;479(2):118-22.Epub2010May21.6.CaoX,XiaoH,ZhangY,ZouL,ChuY,ChuX.1,5-Dicaffeoylquinicacid-mediatedglutathionesynthesisthroughactivationofNrf2protectsagainstOGD/reperfusion-inducedoxidativestressinastrocytes.BrainRes.2010;1347:142-8.Epub2010Jun1.7.HuangL,BiHC,LiuYH,WangYT,XueXP,HuangMCAR-mediatedup-regulationofCYP3A4expressioninLS174TcellsbyChineseherbalcompounds.DrugMetabPharmacokinet.2011;26(4):331-40.8.LiQ,LeiRX,ZhouXD,KolosovVP,PerelmanJM.RegulationofPMA-inducedMUC5ACexpressionbyheparininhumanbronchialepithelialcells.MolCellBiochem.2012Jan;360(1-2):383-91.9.LuanHF,ZhaoZB,ZhaoQH,ZhuP,XiuMY.HydrogensulfidepostconditioningprotectsisolatedratheartsagainstischemiaandreperfusioninjurymediatedbytheJAK2/STAT3survivalpathway.BrazJMedBiolRes.2012May31.