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1Corporationobtainingapproval,thenameofitsrepresentative,andtheaddressofitsmainofficeName:DowChemicalJapanLtd.5Applicant:MichiroKurita,RepresentativeDirectorAddress:2-24HigashiShinagawa2-chome,Shinagawa-ku,Tokyo10ApprovedType1UseRegulationsNamesofTypesofLivingModifiedOrganismsMaizeresistanttoLepidopterapests,andtoleranttoglufosinate,glyphosateandaryloxyalkanoateherbicides(cry1A.105,modifiedcry2Ab2,modifiedcry1F,pat,modifiedcp4epsps,modifiedaad-1,Zeamayssubsp.mays(L.)Iltis)(MON89034×B.t.Cry1Fmaizeline1507×NK603×DAS40278,OECDUI:MON-89Ø34-3×DAS-Ø15Ø7-1×MON-ØØ6Ø3-6×DAS-4Ø278-9)(includingtheprogenylineswhichareisolatedfromthemaizelines,MON89034,B.t.Cry1Fmaizeline1507,NK603andDAS40278,thatcontainacombinationoftheirrespectivetransferredgenes(exceptthosealreadygrantedanapprovalregardingTypeIUseRegulation))ContentofType1UseofLivingModifiedOrganismsProvisionasfood,provisionasfeed,cultivation,processing,storage,transportation,disposalandactsincidentaltothemMethodofType1UseofLivingModifiedOrganisms―152OutlineoftheBiologicalDiversityRiskAssessmentReportI.InformationcollectedpriortoassessingAdverseEffectsonBiologicalDiversity1.Informationconcerningpreparationoflivingmodifiedorganisms5MaizeresistanttoLepidopterapests,andtoleranttoglufosinate,glyphosateandaryloxyalkanoateherbicides(cry1A.105,modifiedcry2Ab2,modifiedcry1F,modifiedaad-1,pat,modifiedcp4epsps,Zeamayssubsp.mays(L.)Iltis)(MON89034×B.t.Cry1Fmaizeline1507×NK603×DAS40278,OECDUI:MON-89Ø34-3×10DAS-Ø15Ø7-1×MON-ØØ6Ø3-6×DAS-4Ø278-9)(hereinafterreferredtoas“thisstackedmaizeline”)istheprogenylinecreatedbyinterbreedingwiththefollowingfourmodifiedmaizelinesusingtheconventionalcrossing.ThisstackedmaizelinewillbecommercializedasaF1hybridline(F1).Duetothe15geneticsegregationtheseedsharvestedfromthisstackedmaizelineincludethestackedmaizelineoracombinationofthegenestransferredintherespectiveparentlinesofthisstackedmaizeline.MaizeresistanttoLepidopterapests(cry1A.105,modifiedcry2Ab2,Zeamays20subsp.mays(L.)Iltis)(MON89034,OECDUI:MON-89Ø34-3)(hereinafterreferredtoas“MON89034”)MaizeresistanttoLepidopterapestsandtoleranttoglufosinateherbicide(modifiedcry1F,pat,Zeamayssubsp.mays(L.)Iltis)(B.t.Cry1Fmaizeline1507,OECDUI:DAS-Ø15Ø7-1)(hereinafterreferredtoas“Cry1Fline1507”)25Maizetoleranttoglyphosateherbicide(modifiedcp4epsps,Zeamayssubsp.mays(L.)Iltis)(NK603,OECDUI:MON-ØØ6Ø3-6)(hereinafterreferredtoas“NK603”)Maizetoleranttoaryloxyalkanoateherbicide(modifiedaad-1,Zeamayssubsp.mays(L.)Iltis)(DAS40278,OECDUIDAS-4Ø278-9)(hereinafterreferredtoas30“DAS40278”)ThesummaryoftheinformationconcerningpreparationofMON89034,Cry1Fline1507,NK603,andDAS40278isdescribedbelow.35(1)Informationconcerningdonornucleicacid1)Compositionandoriginsofcomponentelements3ThecompositionofdonornucleicacidsandtheoriginsofcomponentelementsusedforeachdevelopmentofMON89034,Cry1Fline1507,NK603,andDAS40278isshowninTables1-4(p.4-8).4Table1.OriginsandfunctionsofthecomponentelementsofPV-ZMIR245usedforthedevelopmentofMON89034ComponentelementsOriginandfunctionT-DNAIregionBNote1-RightBorderDNAfragmentcontainingtherightbordersequenceofnopalinetypeT-DNAregionderivedfromAgrobacteriumtumefaciens.TherightbordersequenceisusedastheinitiationpointofT-DNAtransferfromA.tumefacienstotheplantgenome(Depickeretal.,1982;Zambryskietal.,1982).PNote2-e35SCauliflowermosaicvirus(CaMV)35SRNA(Odelletal.,1985)promoterand9bpleadersequence,containingdoubleenhancerregions(Kayetal.,1987).Involvedintheconstantexpressionofthetargetgenesinentiretissuesoftheplantbody.LNote3-Cab5’-terminaluntranslatedleaderregionofwheatchlorophylla/bbindingproteinderivedfromwheat.Itactivatestheexpressionoftargetgenes(Lamppaetal.,1985).INote4-Ract1Intronoftheactingenefromrice(Oryzasativa)(McElroyetal.,1991).Itinvolvestheregulationofexpressionoftargetgenes.CSNote5-cry1A.105GenecodingfortheCry1A.105protein.TheCry1A.105proteinencodedbythecry1A.105gene,whichisusedtodevelopMON89034,isthesyntheticBtproteincomposedofthedomainsIandIIoftheCry1Abprotein,thedomainIIIoftheCry1Fprotein,andtheC-terminaldomainoftheCry1Acprotein.ItisdevelopedtoenhanceinsecticidalactivityagainstthetargetLepidopterapestsbycombinationofdomainsofdifferentBtproteins.TNote6-Hsp173’-terminaluntranslatedregionofthewheatheatshockprotein17.3.Itterminatestranscriptionandinducespolyadenylation(McElwainandSpiker,1989).P-FMV35SpromoterderivedfromtheFigwortMosaicVirus(Rogers,2000).Itinducestheconstitutiveexpressionofthetargetgenesinalltissuesoftheplantbody.I-Hsp70Firstintronofthemaizeheatshockprotein70gene(BrownandSantino,1995).Itactivatestheexpressionoftargetgenes.TSNote7-Rbcs(Zm)Transitpeptideofthesmallsubunitofribulose-1,5-bisphosphatecarboxylaseofmaize.Itcontainsthefirstintronsequence(Matsuokaetal.,1987).Ittransferstheproteinsconnectedwiththedownstreamregiontotheplastids.CS-modifiedcry2Ab2GenecodingforthemodifiedCry2Ab2proteinderivedfromBacillusthuringiensis(WidnerandWhiteley,1989).Inordertoaddarestrictionendonucleasecleavagesite,whichisusedincloning,anasparticacidisinsertedafterthemethionineattheN-terminal,comparedtothewild
本文标题:DAS-40278等4种转基因玉米资料
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