搜索
毕业设计(论文)中文题目：铀矿石冶金菌优势菌株的研究学生姓名：郭勤学号：060552203指导教师：李江专业：生物技术二零一零年六月Graduationproject(paperS)Englishtitle：UraniumminemetallurgyfungussuperioritystrainresearchName:GuoqinNumber:06055203Instructor:LijiangMajor:BiotechnologyJune，2010I摘要嗜酸性微生物是一类生活在极端酸性环境下的微生物，已被广泛用于生物冶金。对取自江西某铀矿堆场的嗜酸性微生物群落优势菌进行了分离和初步的分析与探讨。对采自江西某矿堆浸矿堆的六个样品：S1，S2，S3，S4，S5，S6，以9K+S+Fe液体培养基对其混合菌群氧化铁的效率进行分析，结果发现S3氧化铁的效率最佳。运用双层固体平板培养技术，以琼脂糖为凝固剂，以SJH为底层添加菌，分离S3样品中的微生物，得到6种嗜酸性氧化亚铁硫杆菌（暂定名为：A1，A2，A3，A4，A5，A6），1株氧化硫硫杆菌（暂定名为：B1），3株异养菌（暂定名为：C1，C2，C3）。根据各菌株在不同平板表面的菌落形态特征，镜下观察结果，并结合16SrRNA基因扩增技术对所获得的菌种进行了初步鉴定。群落中占优势的嗜酸性微生物为氧化亚铁硫杆菌(Acidithiobacillusferrooxidans，简称A.f)。对其中一株氧化亚铁硫杆菌（A6）的培养温度、接种培养基初始pH值及接种量对其生长状况的影响做了单因素实验，结果表明，A6氧化Fe2+最快的培养温度为35℃、最适初始pH值为2.3。最佳接种量为10%。初步研究了底物S、Na2S2O3和Na2SO3对氧化硫硫杆菌B1的生长的影响，结果表明最适合B1的底物为Na2S2O3，其次为单质S。关键词：嗜酸性微生物；双层固体平板；氧化亚铁铁杆菌；氧化亚铁硫杆菌东华理工大学毕业设计AbstractIIAbstractTheacidophiliamicroorganismisakindoflifeundertheviolentacidicenvironmentmicroorganism,haswidelyusedinthebiologicalmetallurgy.HascarriedontheseparationandthepreliminaryresearchandthediscussiontotheJiangxisomeuraniummineacidophiliamicroflorasuperiorityfungus.thisarticletopickspilesfromJiangxisomeoresoaksexperiment'ssixsamples:S1,S2,S3,S4,S5,S6carriesontheanalysisinthe9K+S+Feliquidmediatoitsmixbacteriacolonyferricoxide'sefficiency,finallydiscoversS3,theferricoxideefficiencyisbest.Utilizesthedouble-deckedsolidplatingtechnique,taketheagaroseasthepeptizer,takeSJHasthefirstfloorincreasefungus,separatesintheS3samplethemicroorganism,obtains6kindofacidophiliaferrousoxidethiobacilli(A1,A2,A3,A4,A5,A6),1sulphuroxidethiobacillus(B1),3heterotrophicbacterias(C1,C2,C3).Accordingtothedifferentfungusinthedifferentdullsurface'scolonialmorphologycharacteristic,themicrostructure,andunifiesthe16SrRNAgenetoincreasethetechnicalappraisalmoldmushroomspawn.Getstheadvantagetheacidophiliamicroorganismfortheferrousoxidethiobacillus(Acidithiobacillusferrooxidans,iscalledA.f).Toferrousoxidethiobacillus(A6)theraisetemperature,initialPH,thevaccinationquantityhascarriedonthesinglefactordiscussiontoitsgrowthcondition'sinfluence,finallyindicatedthatA6theoptimumtemperaturefor35℃,mostsuitablepHis2.3,themostreasonablevaccinationquantityis10%.Tosulphuroxidethiobacillus(B1)thedifferentsubstratehasdonethepreliminarystudytoitsgrowth'sinfluence,finallyindicatedthattomostsuitsB1thesubstrateistheNa2S2O3nextforS.KeyWords:Acidophiliamicroorganism;Double-deckedsolidplate;16srDNA;Singlefactor东华理工大学毕业设计目录目录摘要....................................................................................................................................................IAbstract...................................................................................................................................................II绪论...........................................................................................................................................................1(1)国内外研究现状...........................................................................................................................1(2)生物冶金发展趋势及前景...........................................................................................................1(3)冶金微生物...................................................................................................................................2(4)浸矿体系中的微生物...................................................................................................................2(5)冶金微生物的多样性...................................................................................................................3(6)环境微生物多样性的研究方法...................................................................................................4(7)双层固体平板法...........................................................................................................................4(8)本文的研究目的和意义...............................................................................................................41试验材料与仪器....................................................................................................................................51.1菌株来源：.................................................................................................................................51.2主要仪器.....................................................................................................................................51.3培养基.........................................................................................................................................51.3.1液体培养基......................................................................................................................51.3.2固体培养基......................................................................................................................62试验方法...............................................................................................................................................72.1活性培养................................................................................................