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Version2.2August2004PurdueUniversityCytometryLaboratoriesIntroductiontoWinMDI2.8(J.Trotter1993-1998)fortheAnalysisofFlowCytometryListmodeDataFilesObjectivesofthistutorial:-Openalistmodedatafile-Createhistograms(single,dual,and3Dhistograms)-Performgating-Performoriginalanalysis-DisplaystatisticsGéraldGREGORI,Ph.D.KathyRAGHEB,M.T.(A.S.C.P.)J.PaulROBINSON,Ph.D.PurdueUniversityCytometryLaboratoriesHansenLifeSciencesResearchBuilding,B050201S.UniversityStreet,WestLafayette,IN47907-2064,USAe-mail:gregori@flowcyt.cyto.purdue.edu….........................................................................................................23Whatwedislike….....................................................................................................24QuickStartin10steps....................................................................................................251.TostartWinMDI2.8.................................................................................................252.Todisplaya2-parameterhistogram.........................................................................253.Tocreatea3Dplot....................................................................................................254.Torotatea3Dgraph.................................................................................................265.Todisplayanewsingle-parameterhistogram..........................................................266.Tocreatearegion…..................................................................................................267.Tocreategates..........................................................................................................278.Todisplaystatistics…...............................................................................................279.Tocreatehistogramoverlay......................................................................................2810.Tocreate3Dviewofhistograms............................................................................28Version2.1June20041I.HowtoInstallWinMDI2.8TheWindowsMultipleDocumentInterfacesoftware(WinMDI)isfreewaredesignedbyJ.Trotter(1993-1998;trotter@scripps.edu)toanalyzeflowcytometriclistmodedatafiles.YoucandownloaditfromtheURL