NameEmail3/7/2012JournalClubH2BubiquitinationmediatedbyRad6/Bre1H2AubiquitinationmediatedbyPRC1KnockoutBmi-1resultsinsignificantlossofH2AubiquitylationandupregulationofHoxc13expression,whereasEZH2-mediatedH3-K27methylationisnotaffected.OurresultssuggestthatEZH2-mediatedH3-K27methylationfunctionsupstreamofPRC1andestablishesacriticalroleforBmi-1andRing1AinH2AubiquitylationandHoxgenesilencing.Thestuctureof2A-HUBnuclearreceptorco-repressor1NR:nuclearreceptorsGPS2:Gproteinpathwaysuppressor2TBL1:transducinbetalike1N-CoR:nuclearreceptorco-repressor1InteractionbetweenNCoR/HDAC1/3componentsand2A-HUB2A-HUBmediatedtranscriptionrepression2A-HUBmonoubiquitinateshistoneH2Ainvitro2A-HUBmonoubiquitinateshistoneH2Ainvitro2A-HUBmonoubiquitinateshistoneH2AinvivoConclusion12A-HUBfunctionsasatranscriptionalrepressor,andcanspecificallymonoubiquitinateH2Ainvitroandinvivo.2A-HUBandN-CoRregulateasubsetofchemokinegeneexpression2A-HUBandN-CoRregulateasubsetofchemokinegeneexpression2A-HUBandN-CoRregulateasubsetofchemokinegeneexpressionTherecruitmentof2A-HUBanduH2AonthetargetgenepromotersConclusion22A-HUBandN-CoRregulateasubsetofchemokinegeneexpression.CoimmunoprecipitationofSpt16andH2A/H2BChIPanalysisoftheendogenousRANTESpromoterChIPanalysisoftheendogenousRANTESpromoterModelConclusion3H2Aubiquitinationmediatedby2A-HUBnegativelyregulatestranscriptionalelongationbypreventingFACTrecruitment.Summary2A-HUBfunctionsasatranscriptionalrepressor,andcanspecificallymonoubiquitinatesH2Ainvitroandinvivo.2A-HUBandN-CoRregulateasubsetofchemokinegeneexpression.H2Aubiquitinationmediatedby2A-HUBnegativelyregulatestranscriptionalelongationbypreventingFACTrecruitment.Thanksforyourattention!