17免疫组化技术应用中的影响因素分析

免疫组化技术应用中的影响因素分析冯静洁,吴玉玉,齐晓薇,张绪森　分析免疫组化操作过程中的影响因素、探讨操作及改进方法。　选取我院免疫组化病例1238例,总结分析操作过程及各种影响因素。　染色结果良好,与临床病理相符或基本相符1126例(90.9%);脱片42例(3.3%);非特异染色37例(2.9%);染色弱29例(2.2%),阴性者5例(0.4%)。　影响免疫组化结果的因素很多,需注意各个环节的操作规范才能获得满意的免疫组化结果。免疫组织化学;染色;抗原修复;质量控制R446.6A1008-7044(2006)04-0284-02AclinicalanalysisofthetechnicalskillofimmunohistochemistyFENGJing-jie,WUYu-yu,QIXiao-wei,etal.(DepartmentofPathology,WuxiFourthPeoplesHospital,Jiangsu214062,China)AbstractObjectiveTostudyinfluentialfactorsduringoperatingimmunohistochemistryandimprovementmethods.Methods1238casesofimmunohistochemistryweresummarizedandanalyzed.ResultsTherateofgood-stainingwas90.9%(1126/1238),non-specialstainingwere37caseswiththerateof2.9%,weak-statiningwere28casesand5casesnagtive.ConclusionManyfactorscaninfluencetheresultofimmunohistochemistry.Attentionshouldbepaidtoeachprocesswhenimmunohis-tochemistryisoperated.KeywordsImmunohistochemistry;Staining;Antigenrestore;Qualitycontrol,,,,,1999,20021200510,214062(1970-),,,,1238,11.1　材料　1.2　方法　(1):,,,(APES)(2):586024h(3),(4):9810010min,10min(0.01%,pH6.0)(5),PBS3min3(6),4(7)PBS:3min3(8),,[4],,,MRI[4],(1)(2)(3),(2448h)[5](4),,,,(5)CX,(6),,,5%,,[1],,.[M].:,1984.29.[2],,,.[J].,2000,10(3):164.[3],,,.[J].,2000,10(3):137.[4],,.[J].,2003,8(4):222.[5],,.[M].:,2001.978.(收稿日期:2005-10-27)28420067244JHuaihaiMed,Jul.2006,Vol.24,No.430min,PBS3min3(9)DAB,(10),,,,,21238,,1126,90.9%;423.3%,1,();,;37,2.9%,;28,2.2%,;5,0.4%,33.1　取材、脱水、浸蜡、固定　1cm1cm0.2cm,,,,,10%,,15min,824h,,3.2　切片与烘片　,,,,,,,,34m,,,,586024h,3.3　抗原修复方法　0.01%(pH6.0),,ERPRKi-67EDTA,9810010min10min20min,CD10CD15CD56TDTCD23CD5,5min,CD4,,,3.4　规范的操作规程及娴熟的技术和高度责任心　,,,,,,3min3,,,(,):(1)41h,(2)30min,37,,,,,,3.5　显色系统　DAB,,,,,DAB,,5mg/ml,510min,,,,,,,,DAB,,,,,,[1],,,.()[J].,2003,10(5):310-311.[2],,,.()[J].,2003,10(6):371-372.[3].[J].,2005,29(4):241-243.[4],,.[J].,2001,23(6):392-393.[5],.[M].:,1997.129-160.[6],.[M].:,2001.354-505.(收稿日期:2006-02-05)28520067244JHuaihaiMed,Jul.2006,Vol.24,No.4