配制0.2M磷酸缓冲液的方法

0.2M磷酸缓冲液磷酸缓冲液液PH5.7--8.0(0.2M)甲液：NaH2PO4·2H2O：Mr=156.03，0.2mol/L溶液为31.21g/L，NaH2PO4·H2O：Mr=138.01，0.2mol/L溶液为27.6g/L。乙液：Na2HPO4·2H2O：Mr=178.05，0.2mol/L溶液为35.61g/L，Na2HPO4·7H2O：Mr=268.13，0.2mol/L溶液为53.624g/L，Na2HPO4·12H2O：Mr=358.22，0.2mol/L溶液为71.64g/L。磷酸缓冲液液PH5.7--8.0(0.2M)PH甲液X(ml)乙液Y(ml)PH甲液X(ml)乙液Y(ml)5.793.56.56.945555.8928739615.990107.13367687.712.37.228726.185157.323776.281.518.57.419816.377.522.57.516846.473.526.57.613876.568.531.57.71090.56.662.537.57.88.591.56.756.543.57.97936.8514985.394.7根据要求的PH值,取X毫升甲液与Y毫升乙液,混合均匀即得.