双岐杆菌RAPD基因指纹图谱与耐药谱分析比较-双岐杆菌R

WHOHAI,,,HAI,,HAI,,ICT,ICT,HAI,ICT,MacELISAMacELISA,ICTMacELISA,,,,,;5min;,;,,1;,,MacELISA,,,,,MacELISA,,,,,,,,ICT,,,,,ICT1SangCT,HoonLS,CuzzubboA,etal.Clinicalevaluationofarapidimmunochromatographictestforthediagnosisofdenguevirusinfection.ClinicalandDiagnosticLaboratoryImmunology,1998,5:4072,,11:,1995:394(2000-01-21)1004-8685(2000)03-0269-03RAPD1211111113(1116622;2;3)AP-PCR204RAPD,,4RAPD,:;;RAPDStudiesonBifidobacteriumWithRapdFingerprintingAndAntibiogramöZhangLiJuan,TanHongLi,etal(FacultyofMedicalLaboratoryofDalianUniversity;Dalian116622)AbstractFourspeciesofbifidobacterium(totalof20strains)werestudiedbyRAPDmethodandanalysisoftheantibiogram.ThroughComparativetheRAPDfingerprintingwefoundmovediversityamongthem,WhichmakeiteasyforustoidentifyBifidobacteriumfromthreeotherBifidobacterium.ButtherewerenotobviouslyrelationbetweentheRAPDfingerprintingandantibiograminthesestrains.Keywords:Bifidobacterium;Antibiogram;RAPDFingerprintingQ755ARAPDPCR,96220006103ChineseJournalofHealthLaboratoryTechnology20001Vol1101No131,DNA1Sangon199610208642RAPD,,:1111:20112DNA:20,(50mgöml82Ll1TAE)371h,20Ll012gömlK(pH718,2%SDS,40mmolTris-HCl,4mmolCaCl)5590min,0102molöLEDTACa2+,ö,,70%,TE113PCR:2Sangon,1(OPL1)17,5q-TGTTCGTGCTGTTTCGT-3q,G+3=47%,2(OPL2)10,5q-AGCGGGCCAA-3qG+C=70%25Ll:2Ll40mMMg2+;015LlTagDNA;215Llbuffer;2Ll50LmolöL,50LmolöLdNTP215Ll;25Ll,25LlPTC-1007MPCR()PCR,1%(EB015Lgöml)(5Vöcm)2h,114:22211RAPD:2(OPL2)204DNA1(OPL1)2039DNA,1411288203DNA794,478,3664,DNADNA1RAPD1114OPL1RAPDDNA81288,1202,1047,891,794,478,396,221,1416794,478,36641288,1202,1047,891,794,478,36621288,891,794,478,3661204RAPD(OPL1)MarKer1632,517,396,298,221212:20122,3220(%)-50-157010-5--75551550-40-35-545-202585-10045305510090551005203201RSRSSSRRRRRR2RmRmRRRRmSmR3RmRRSRRRRRSS4RSRRSRRmmRSS5mSRmSRRRRRSS6mmRmRmRRmRmm7RSRRSmRRRRmR8RSRRSmRRRRSS1RSRSSRRRRRSS2RmRRSRRRRRSm3RSRRRRRRRRSm4RSRSSSRSmRSS5mSRRSRRRRRSR6RmRmSmRRmRSS1RmRmRmRRmRSS2RmRmRmRRRRSS3RmRRSRRRmRSS4RSRmSmRRmRSm1RmRRSRRRRRSS2RmRmRRRRmRmm07220006103ChineseJournalofHealthLaboratoryTechnology20001Vol1101No1313311RAPD:2042DNA,23DNA,4,3DNA,,,DNA,RAPDAp-PCR312:,G,3.4313RAPD:,4RAPD,,204RAPD,RAPD,,511DNA1,1998,19(3):118-220211:,1994:72-873,11,1996,8(5):4-64,1()1,1994,6(5):59511,1988:443(1999-11-03)1004-8685(2000)03-0271-02112(1471003;2450052),2%,ELISAHBsAg2%30min,HBsAg100%2%:;;;HBsAgStudingthesterilizingeffectofGlutaraldehydeonthedentalinstrumentsincommonuseöHuangMin,ZhangHuimin,MinYueqin(AffiliatedHospitalofHenanProvinceAcupuncture&MoxibustionandMassageschool,Luoyang471003)AbstractObjectiveTofindoutarapidandeffectivesterilizingmethodtocutoffthechannelsofcrossinfectionofcommon-useddentalinstrument.MethodsStudingthesterilizingeffectonthedentalinstrumentsincommonuseafterimmersionwith2%glutaraldehyde.ResultsAfter30minutesimmersionwith2%glutaraldehyde,inactivationrateofHBsAgis100%.Conclusionsusing2%glutaraldehydeasadisinfectanttosoakinstrumentisarapid,idealandeffectivemethod.Keywords:dentalinstrument;sterilizing;glutaraldehyde;HBsAgQ93-334A,(HBsAg),,17220006103ChineseJournalofHealthLaboratoryTechnology20001Vol1101No131