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281Vol128,No11JIANGXICOTTON20062Feb1,2006,,,(,415101):,,:;;DNA:S562103512:A:1006-0499(2006)01-0003-03,()mRNAcDNA,,,,,1111,DNA,DNA(400/cm2)()(mNRAcDNADNA),,cDNADNA:,,DNA;DNA,DNA,DNA,112,,,;,,,,;,,::RNA,DNA,,3:2005-11-072211,cDNA,,,AharoniA1701cDNA,480,,GuteramanADNASekiMetal,()cDNA,1300cDNA,cDNA,,1300cDNA44,19,3010,12DREB1,6[14]212,DNA,DNADNA,BevanM4119Mb,418Kb,54%GenBank,56%GenBankEST,20%,5:DNADNA,,19%,,,213,NorthernReal-timePCRmRNAcDNADNASchenaetal45cDNA(14,31EST),26,CAB1500WangYh,HammondJP,YuLX/,MichaeLSchaffer115211d6h11%,,,,,cDNAShinozaki7000cDNAABA,22ABA,ABA;,5341,9DREDRE,[36]214,,StandfordDavisDNAORFORF,ORF20ORF,4vol128,No11:20062DNA,ORF,ORFDNA,[5]215()EST,,SuzukiM35S:VP1353VP1/ABA73%VP1ABA,ABAVP1AbeHrd22MYCMYBrd22Sinvany-VillaloboG70-mer[7]3,,,RNA,,,,,,:,cDNARNA(l0ugRNA5OOngmRNA),RNARNA,;,;,,,,,,,,DNA,,[1],,,1[J]1,2004,2:9121[2],,,1[J]1,2003,29(1):1591[3],1[J]1,2002(4):30351[4],1[J]1,2004,14(6):70721[5],,,1[J]12001,18(5):121[6],,1[J]1,2003,7(2):44491[7],,1[J]1,2002,31(1):2661ResearchProgressandprospectsofusinggenepermutationtechniqueincropsZHANGZhi-gang,YANGXiao-ping,MEIZheng-ding,LIUKai-zhi(Hunanprovincecottonscienceresearchinstitute1,Changde415101,China)Abstract:Thisarticlesummarizedtechniqueprincipleanddesigntheoryofgenepermutationtechnique.Italsointerducedchinesegenepermutationtechniqueresearchprogressfromfiveaspects.Theseincludingfindingnewgene.determiningenegrouporder.determininggeneexpresslevelandtransgenefarmproducts.atthesametimelookforwardchinesegenepermuta2tionresearchpracticeprospects.Keywords:genepermutation;crop;DNA5vol128,No11:20062