基因芯片技术在分支杆菌研究中的应用

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309(100091)/R52,19852000,8001000,3001993(WHO),1998,22,,2000,4;500,200;15,11,;,46%1990,88.7%,6.4%,MOTT4.9%,MOTT15.4%,20001221,1996StevenFodor,,,1(Genechip)(Biochip)DNA(DNAchip),CPU,,cDNA,(Microarray)2,,,DNA,DNA,,,:PCR,DNARNA,,316SrRNArpoB,,,,,,[19],RNA(rpoB)14121020025ChinaJournalofModernMedicineVol.12No.10May.2002©1995-2006TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.;-(katG)(inhA)(ahpC)-(kasA);(pncA);embABC;S12(rpsL)16SrRNA(rrs);DIAA(gyrA)B(gyrB),,1998Gingeras[10]rpoB,,,,,1083rpoB16SrRNA,,rpoB705bp80,16SrRNA180bp21,rpoB705bp,14.3%()4.1%();4.2%(3.7%),,70,1/51/3,;rpoB16SrRNA13,41,,2,,rpoB16S,,;rpoB16S,;rpoB,16S13;,81,76;5,3rpoB,16SrpoB,,rpoB705bp,44RFP,41rpoB81240,53152651335%,28%25%,16,3RFP1999Troesch[11]16SrRNA(200bp)2770,:Conspicu2umInterjectumShimod2ei,,4,1;4,116SrRNA,,,rpoB(200bp)115RFP2412©1995-2006TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.,13RFP,2361999Head[12],DNA,9RFPrpoB531526,4h,,(16S),;,,;,,(01J020),,,,,,,1998H37Rv,,,,;,Raychaudhuri[13,14]DNAIS6110,,;,,,,,,1,,,.Molecularmechanismsofdrugresis2tanceinM.tuberculosisclinicalisolates.ChineseMedicalJournal,1999;112(6):5245282,,,.katGinhA.,1998;23(5):3623673,,,.PCR-KatG.,2000;18(1):9114,,,.AhpC.,1998;21(11):135,,,.pncA.,2000;23(1):40426,,,.embB.,2001;24(4):137,,,..,1996;19(6):3428,,,.16SrRNA.,1998;21(9):5969TakiffHE,SalazarL,GuerreroC,etal.Cloningandnucleotidese2quenceoftheMycobacteriumtuberculosisgyrAandgyrBgenesanddetectionofquinoloneresistancemechanisms.AntimicrobAgentsChemother,1994;38:77310GingerasTR,GhandourG,WangE,etal.SimultaneousgenotypingandspeciesidentificationusinghybridizationpatternrecognitionanalysisofgenericmycobacteriumDNAarrays.GenomeRes,1998;8:43544811TroeschA,NguyemH,MiyadaCG,etal.Mycobacteriumspeciesi2dentificationandrifampinresistancetestingwithhight-densityDNAprobearrays.JClinMicrobiol,1999;37(1):495512HeadSR,ParikhK,RogersYH,etal.Solid-phasesequencescan2ningfordrugresistancedetectionintuberculosis.MolecularandCellularProbes,1999;13:818713RaychaudhuriS,StuartJM,LiuX,etal.Patternrecognitionofge2nomicfeatureswithmicroarrays:sitetypingofMycobateriumtu2berculosisstrains.ProcIntConfIntellSystMOlBiol,2000;8:28629614,,,.16000(rPA16).,2000;10(12):4142(2001-09-27):1271063410:©1995-2006TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.

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