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C2005,35(6):551~557551*****(,266003;/,100050;,100050;304,100050;,100050;301,100050),224,.,,.(r20.95),1pmol/L.,,,..52~112pmol/L,3~70pmol/L,41.9~81.4pmol/L;0.8~3pmol/L,1.2~2.5pmol/L,0.6~2.64pmol/L.,,,.,,,.2005-04-24,2005-09-27*863(:2002AA2Z2004,2002AA2Z343B2002BA711A01-11)**,E-mail:zhangjT@imm.ac.cn,dugh@imm.ac.cn,.,,().,[1~3],[4],[5~7],(leanbodymass)[8,9],SCIENCEINCHINASer.CLifeSciences552C35,,[10,11],,,,[12,13].,.,,.,,,.,,.:,,;,.,,.,[14],,,.22460~80,.11.1224(60~80).111,69.15±4.8,60~6529,66~7035,71~7540,76~807;113,58.32±5.7,60~6555,66~7037,71~7519,76~802.1.,−20.,.1.2(FITC-E)Sigma-Aldrich(:E1653,E6507,281778,T1500,E1024,USA).,(FITC-T)Panvera(:p2719,p3010,USA).1.3Dy-2003(,),Scan-3(,).1.4(1)::1%,(10mmol/L),70.()2mL,4h,37.[15].:(750pmol/L),.500µm,100µm,60ms.4,[16].(2)::5,6,300,100,30,10,3pmol/L(/[17],);,FITC-T(10pmol/L),3730min,PBST(+0.05%20)3,5min.,488nm,532nm.:6SCIENCEINCHINASer.CLifeSciences6553,3,100000,30000,3000,300,30,3pmol/L;,FITC-E(10pmol/L),.,.2,,.,.(3):3,30,300pmol/L(n=6),.1,16%,;10%,.(4):10,9,10pmol/L;1,3pmol/L10pmol/L..2.23.,,..(5):,ImageAnalyzer1.0(RFU),.,,,.Spss11.0Sigmaplot8.0.4,,,..66~75,.52~112pmol/L.3~70pmol/L,41.9~81.4pmol/L.2.325,,,.,0.8~3pmol/L.1.2~2.5pmol/L,0.6~2.64pmol/L..2.11,.,,.1a)()/pmol·L−1()/pmol·L−133030033030012.2730.36327.203.4931.64337.0821.6924.56293.951.8635.96279.9433.3728.97357.123.0020.47298.9543.2926.98300.122.3936.51287.5352.2430.66275.584.2828.02335.7164.1326.11351.881.4532.18313.60(X)2.8327.94317.642.7530.80308.80(SE)0.371.0013.490.432.429.88(CV)13.07%3.58%4.25%15.64%7.86%3.20%(Bias)b)5.67%6.87%5.88%8.33%2.67%2.93%a)3,30,300pmol/L()()(n=6);;.b)|−|/(a).5,6,5,,10pmol/LFITC-T,.(b)2(a).6,3,6,,10pmol/LFITC-E,.(b)SCIENCEINCHINASer.CLifeSciences6555310.6,10pmol/LFITC-TFITC-E,4(mean±SE)111(60~65n=29,66~70n=35,71~75n=40,76~80n=7);113(60~65n=55,66~70n=37,71~75n=19,76~80n=2).P0.05,P0.013,,,,5(mean±SE)111(60~65n=29,66~70n=35,71~75n=40,76~80n=7);113(60~65n=55,66~70n=37,71~75n=19,76~80n=2).[18].()/(SHBG).,SHBG(60~89)(60~89)(fT)(fE2)(fT)(fE2)/pmol·L−152~1120.8~33~701.2~2.5/pmol·L−128.7~192.7[18,25,26]1.1~3.9[18]3.88~30.0[20,21,27]0.12~2.04[21],.mean±SE(mean−SE~mean+SE),.,[19~21]..,.(),.,,().(12),(r20.95),(10%)(1pmol/L),,,,,.224,.,60~85,[18,22~24].,,(45),,.,(5),,,,.,.(1~2),..2,(),.,.,.,,,,,..1DunningAM,DowsettM,HealeyCS,etal.Polymorphismsas-sociatedwithcirculatingsexhormonelevelsinpostmenopausalwomen.JNatlCancerInst,2004,96(12):936~9452VermeulenA,KaufmanJM,GoemaereS,etal.Estradiolineld-erlymen.AgingMale,2002,5(2):98~1023HarmanSM,MetterEJ,JordanDT,etal.Longitudinaleffectsofagingonserumtotalandfreetestosteronelevelsinhealthymen.JClinEndocrinolMetab,2000,86(2):724~731[DOI]4SadowskyM,AntonovskyH,SobelR,etal.Sexualactivityandsexhormonelevelsinagingmen.IntPsychogeriatr,1993,5(2):181~1865MullerM,AlemanA,GrobbeeDE,etal.Endogenoussexhor-monelevelsandcognitivefunctioninagingmen:Ithereanopti-mallevel?Neurology,2005,64(5):866~8716CunninghamCJ,SinnottM,DenihanA,etal.EndogenoussexhormonelevelsinpostmenopausalwomenwithAlzheimer’sdis-ease.JClinEndocrinolMetab,200186(3):1099~1103[DOI]7HogervorstE,DeJagerC,BudgeM,etal.Serumlevelsofestra-diolandtestosteroneandperformanceindifferentcognitivedo-mainsinhealthyelderlymenandwomen.Psychoneuroendocri-SCIENCEINCHINASer.CLifeSciences6557nology.2004,29(3):405~421[DOI]8ForbesGB,ReinaJC.Adultleanbodymassdeclineswithage:Somelongitudinalobservations.Metabolism,1970,19:653~6639GallagherD,VisserM,DeMeersmanRE,etal.Appendicularskeletalmusclemass:Effectsofage,gender,andethnicity.JApplPhysiol,1997,83:229~23910MarcusR.Skeletalaging.Understandingthefunctionalandstructuralbasisofosteoporosis.TrendsEndocrinolMetab,1991,2:53~58[DOI]11StoneK,BauerDC,BlackDM,etal.Hormonalpredictorsofbonelossinelderlywomen:Aprospectivestudy.TheStudyofOsteoporoticFracturesResearchGroup.JBoneMinerRes,1998,13:1167~117412KumagaiS,KaiY,SasakiH.Relationshipbetweeninsulinresis-tance,sexhormonesandsexhormone-bindingglobulininthese-rumlipidandlipoproteinprofilesofJapanesepostmenopausalwomen.JAtherosclerThromb,2001,8(1):14~2013CouillardC,LemieuxS,MoorjaniS,etal.Associationsbetween12yearchangesinbodyfatnessandlipoprotein-lipidlevelsinmenandwomenoftheQuebecFamilyStudy.IntJObesRelatMetabDisord,1996,20:1081~108814MacBeathG,SchreiberSL.Printingproteinsasmicroarraysforhigh-throughputfunctiondetermination.Science,2000,289:1760~176315AfanassievV,HanemannV,WolflS.PreparationofDNAandproteinmicroarraysonglassslidescoatedwithanagarosefilm.NucleicAcidsRes,2000,15(28):E66[DOI]16RenaultJP,BernardA,JunckerD,etal.Fabricatingmicroarraysoffunctionalproteinsusingaffinitycontactprinting.AngewchemInhtEd,2002,41:2320~2323[DOI]17OchiY,ShiomiK,HachiyaT,etal.Dextran-coatedcharcoaltechniquetomakethehormone-freeserumasadiluentforstan-dardcurveofradioimmunoassay.EndocrinolJpn,1973,20(1):1~718BeldAW,JongFH,GrobbeeDE,etal.Measuresofbioavailableserumtestosteroneandestradiolandtheirrelationshipswithmus-clestrength,bonedensity,andbodycompositioninelderlymen.JClinEndocrinolMetab,2005,85(9):3