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3220045(FENXIHUAXUE)ChineseJournalofAnalyticalChemistry555756011221,3311(,116023)2(,116023)3(,)3(polymethalmethacrylate,PMMA)1.13810-40.127910-4-0.852010-4cm2v-1s-1,,,,2003206203;2003211210(No.20275039)(No.20035010)(No.20299035)11,,2345,6,7(CE)89,,,10CE,,,2090,1115,,,,,CE,,Ichiki4,:3,;22.1(),(C23H19ClF3NO3,),(),0.2mol/LNaH2PO40.2mol/LNa2HPO4,4.75mLNaH2PO420.25mLNa2HPO4,0.85gNaCl,100mL,1mol/LNaOHpH7.4,50mmol/LPBS(phosphatebufferedsaline)0.4m1g100mL0.85%,1%42.2XSB21A3()10,CCD(Mintron,),CCDSDK2000(Tuma)K81C235(KILOVAC)(30kV,),VisualBasic(Microsoft)4.5cm,100m,50m,0.4cm,0.1cm2.3,3(1):1mL1%,5mL,,,04,3000r/min,20min,,,pH7.4PBS(10mmol/L),04,1500r/min,5min,,2pH7.4PBS(10mmol/L),108/mL13Table1ThethreegroupsofcarptreatedwithdifferentmethodsGroupnumberPreparation1Group1Thecarpwasfeedinawatertankwithwaterclarifier2Group2,6g/kgThecarpwasfeedinawatertankwithwaterclarifierandwasinjected6g/kgLambda2cyhalothrinbintomuscle3Group3,6g/kgThecarpwasfeedinawatertankandwasinjected6g/kgLambda2cyhalothrinbintomuscle2.413L,,13L,,,133,3Fig.1Histogramofvelocityofredbloodcellextractedfromdifferentgroups.Insetistherelationshipofvelocityvs.appliedelectricfields1.1(group1);2.2(group2);3.3(group3),,,CCDCCD25/s,,,PHO2TOSHOP,2533.1,280V/cm,,,,280V/cm1610mmol/LPBSPMMA(polymethylmethacrylate)1.837910-4cm2/(Vs)3.213,,VonSmoluchowski1785532,ep=e,ep,,e,,,13,,32F,(25)12323Table2Apparentmobilityandelectrophoreticmobilityofredbloodcell(incm2V-1s-1)Groupnumber(10-4)Electroosmoticmobility(10-4)Apparentmobility(10-4)Electrophoreticmobility1Group.10.7001.1382Group.21.8381.710.12793Group.32.69-0.852(Group2,Group3),12.43.8,3,,,,,,N2,23(A)(B)Fig.2Cellsdifferentiationbyelectrophoreticmobilityandmorphologyobservation(A)andtoppanelshowsthreepeaksfittedwithnormalfunction(B)A.1232B123(panelBarethephotographsofthreegroupscells.number1,2,3correspondtogroup1,2,3)AEP,,23,3.3,(),,,2A3313,22B2B12,,312,,,9555:4:(1),;(2)References1FanJingyu(),WeiZhimei(),LiuZhi().ProgressinBiochemistryandBiophysics(),1983,2(4):40462SimionescuM,SimionescuN.CellBiol.Rev.,1991,25:5803VanOssCJ.in:BauerJ.(Ed.).CellElectrophoresis,CRC2Press,BocaRaton,FL,1994,Chapter114TakanoriI,TatekazuU,SatomiS,TomokoO.Electrophoresis,2002,23:202920345LinKechun().Biophysics().Beijing():PeoplesEducationPress(),1981:2672826HashimotoN,FujitaS,YokoyamaT,OzawaY,KingstsuI,KurosakaD,SabolovicD,SchuettW.Electrophoresis,1998,19:122712307LiuJian(),LiAnli().ChineseJournalofBiomedicalEngineering(),1990,9(3):1611918ArmstrongDW,GirodM,HeL,RodriguezMA,WeiW,ZhengJ,YeungES.Anal.Chem.,2002,74:552355309MaoHuisheng(),HuLandian(),MaQiang(),LiAnzhi().ChineseJournalofBiomedicalEngineering(),1994,13(2):17117410YanZongyi(),ShanHuixian().ChineseJournalofHermorheology(),2001,11(1):1411ReysesDR,IossifidisD,AurouxPA,ManzA.Anal.Chem.,2002,74:2623263712AurouxPA,ReyesDR,IossifidisD,ManzA.Anal.Chem.,2002,74:2637265213InoueI,WakamotoY,MoriguchiH,OkanoK,YasudaY.Lab.OnaChip,2001,1:505514ChengJ,SheldonEL,WuL,HellerMJ,ConnellJP.Anal.Chem,1998,70:2321232615GascoyneP,MahidolC,RuchirawatM,SatayavivadJ,WatcharasitP,BeckerF.Lab.OnaChip,2002,2:707516LaurieELocascio,CatherineEPerso,ChengSLee.JournalofChromatographyA,1999,857:27528417VonSmoluchowskiM.in:GreatzL.(Ed.).HandbuchderElektrizittunddesMagnetismus,Barth,Leipzig,1921,2:366ElectrophoreticMobilityofCarpErythrocyteonMicrofluidicChipYuLinfen1,GaiHongwei1,QiHongli2,GuiYuanming2,MaYinfa1,3,LinBingcheng311(DalianInstituteofChemicalPhysics,Dalian116023)2(DalianFisheriesUniversity,Dalian116023)3(UniversityofMissouriatRolla,US)AbstractTheelectrophoreticmobilityofthreegroupsofcommoncarperythrocyteonpolymethalmethacrylatechiphasbeenstudiedbysinge2cellimaging.Thevaluesofcellelectrophoreticmobilitywere1.13810-4,0.127910-4,-0.852010-4cm2V-1s-1respectively.Itisdemonstratedthatthevaria2tionofthecellmigrationvelocityisoriginatedfromthechangeofwaterqualityandLambda2cyhalothrin.Thecellelectrophoreticmobilitybeingusedasaparameterforcellclassificationhavealsobeendiscussed.KeywordsMicrofluidicchip,cellelectrophoresis,carperythrocyte(Received3June2003;accepted10November2003)06532