搜索
32Vol.3No.219996LifeScienceResearchJun.1999:100727847(1999)0220092204a,(,410078):,LL:;;;;:Q78:ANovelGeneticEngineeringDrugsTANGDong2sheng,XIAJia2hui(NationalLaboratoryofMedicalGenetics,HunanMedicalUniversity,Changsha410078,Hunan,China)Abstract:Theauthorintroducedtheproducingmethodsandapplicationsofgeneticengi2neeringvaccinesanddrugsproducedbytransgenicplantsortransgenicanimalmammarybioreator,andanalyzedtheadvantagesandproblemsofthegeneticengineeringdrugs.Theauthoralsoreviewedtheclinicaltrailandapprovalofthegeneticengineeringdrugsproducedbymicrobialfermentationsandanimalcellcultures.Keywords:geneticengineeringdrug;transgenicplant;vaccine;transgenicanimal;mam2marybioreactor,,40LL,L1,,,,a:1998209221;:1999201224:(39830200)L:(19632),,,,,:;(19372),,,,,,:L©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.,,1990L,,,,L1.1:LT2DNA,,[1],,L[2]LLL1.2,[3]:1),;,;,L2),,L3),,,,L,L4),,LLL,:1)L,[4]L2)L(),L,L3),L1.3L1.3.1:1,(1990)SpaA,AmtzenL1992Mason[4]L[2]L1.3.2:1995Mason,,LAmtzen,[5]L,,,,L,1995L1.3.3:,[6]L1.3.4:4,[7]L392:©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.2,,,L2.1,,,,L2.2,:1),,,L2),L3),,L4)L,,:1),L2),[8]L2.31988,:C,,IXC,CIX[9]L3L,199548,199760,20%,1982,40,L12,10,8L1997I7,II18,III10,39(),,IL(interleukin,)25IL215IL28IL28L491999©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.1Table1ListofgeneticengineeringdrugsinclinicaltrailandapprovalclassficationÉclinicalphaseÉÊclinicalphaseÊËclinicalphaseËapprovalÏÑÐSCFTPOG2CSFM2CSFGM2CSFEPOSODEGFFGFEGFHGH9IFNA1b,A2a,A2b,An3,B,B1b,C,C1bMAb6tPAIL21AIL21BIL23IL26IL22IL24IL212IL211CEAHSVLymeHBVHCVIGF21HböÉTNFSCF:stemcellcolonyfactor,TPO:thrombopoietin,G2CSF:gran2ulocytecolonystimulatingfactor,M2CSF:macrophagecolonystimulatingfactor,EPO:erythropoietin,SOD:superoxidedismutase,EGF:epidermalgrowthfactor,FGF:fibroblastgrowthfactor,lHGH:humangrowthhormone,IFN:interferon,MAb:monoclonalantibody,tPA:tissueplasminogenhactivator,CEA:carcinoembryonicantigen,HSV:herpessimplexvirus,HBV:hepatitislBvirus,HCV:hepatitisCvirus,IGF:insulin2likegrowthfactor,Hb:hemoglobin,TNF:tumornecrosisfactor1:[1]FULLNERKJ,LARAJC,NESTEREW,etal.PilusassemblybyagrobacteriumT2DNAtrans2fergenes[J].Science,1996,273:110721109.[2]TURPEUTH,REINLSJ,CHAROENVITY,etal.Malarialepitopesexpressedonthesurfaceofrecombinanttobaccomosaicvirus[J].BioöTechnology,1995,13:53257.(101)592:©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.HPRTgenearenecessaryforitsexpressioninembryonicstemcells[J].ProcNatlAcadSci.USA,1990,87:429924303.[8]ASKEWGR,DOETSCHMANT,LINGRELJB.Site2directedpointmutationsinembryonicstemcells:agenetargetingtag2and2exchangestrategy[J].MolCellBiol,1993,13:411524124.[9]WUH,LIUX,JAENISCHR.Doublereplacement:strategyforefficientintroductionofsubtlemu2tationsintothemurinecolla21genebyhomologousrecombinationinembryonicstemcells[J].ProcNatlAcadSciUSA,1994,91:281922823.[10]GUH,ZOUYR,RajewskyK.IndependentcontrolofimmunoglobulinswitchrecombinationatindividualswitchregionsevidencedthroughCre2loxP2mediatedgenetargeting[J].Cell,1993,73:115521164.[11]JUNGS,RAJEWSKYK,RADBRUCHA.Shutdownofclassswitchrecombinationbydeletionofaswitchregioncontrolelement[J].Science,1993,259:9842987.[12]ALADJEMMI,RODEWALDLW,KOLMANJL,etal.Science,1998,281(5379):100521009.[13]ORBANPC,CHUID,MARTHJD.Tissue2andsite2specificDNArecombinationintransgenicmice[J].ProcNatlAcadSciUSA,1992,89:686126865.[14]LAKSOM,SAUERB,MOSINGERBJr,etal.Targetedoncogeneactivationbysite-specificre2combinationintransgenicmice[J].ProcNatlAcadSciUSA,1992,89:623226236.(95)[3]GARYHT.Plantbiotechnologyanddevelopingcounties[J].TrendsinBiotechnology,1995,13:4042409.[4]MASONHS,LANDMK,ARNTZENCJ.ExpressionofhepatitisBsurfaceantigenintransgenicplants[J].ProcNatlAcadSciUSA,1992,89:11745211749.[5]HAQTA,MASONHS,CLEMENTSJD,etal.Oralimmunizationwitharecombinantbacterialantigen?producedintransgenicplants[J].Science,1995,268:7142716.[6]McGARVEYPB,HAMMONDJ,DIENELTMM,etal.Expressionoftherabiesvirusglycopro2teinintransgenictomatoes[J].BioöTechnology,1995,13:148421487[7]MAYGD,AFZAR,MASONHS,etal.Generationoftransgenicbanana(musaacuminata)plantsviaagrobacterium2mediatedtransformation[J].BioöTechnology,1995,13:4862492.[8]RIJNKELSM,KOOIMANPM,KRIMPENFORTPJA,etal.Expressionanalysisoftheindivid2ualbovineB2,As22andJ2caseingenesintransgenicmice[J].BiochemJ,1995,311:9292937.[9].[J].,1997,17(6):2216.1012:©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.