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©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:2008212223;:2009204210:(06ZD1105):(19712),,,,Email:heping@swust.edu.cn:100421656(2009)0720937208DNA,3,,,(,621010):DNA,,DNA:;DNA;;:O657.1;O657.3:A,[1,2],,DNA[3],RNA,DNA,,DNA1953WatsonDNA[4],DNA,DNA,,[528],DNA,[9]DNA1DNA[1,10]1.1(1)[11](1):DNA,;(2):DNA;(3):DNA,DNA1DNAFig.1ThebindingmodeofthepharmaceuticalmoleculesandDNA,,DNA,©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[11,12]1.3,DNADNADNADNADNADNA[13][14]2DNADNA,2[14,15],,2,,,DNA,,,pH,DNA[16219]3DNA,DNA,,,DNA[20227]3.1,DNA,,,[28233],DNADNADNA[34240],DNA,,2[41246]DNA[47,48]2DNA,DNA(af)DNADNA,2DNAFig.2Cyclicvoltammogramanddifferentialpulsevoltammogram(insert)oftheinteractionbetweenethylferulateandDNA.Bard[49]DNA839©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[50]DNA(CT2DNA)CT2DNA,,,CT2DNA,DNA,DNA,DNADNA,DNA,,DNA,Tian[51]DNADNA,DNA,[52],DNA,,[53,54],DNA3.2DNADNA,,,[55278]3.2.1DNA,DNA,260nm,DNALong[55],DNA,DNA,[56258],[59262];DNA,,[63,64][65]2Cu2+DNA,pH7.4Cu2+DNA2Cu2+,2Cu2+DNALin[66]DNA(HS2DNA),HS2DNA,,HS2DNA,3.2.2,DNA,[67],DNA,,[68,69];,DNA[70272]DNADNA,DNA,,DNA[73](AO)B2RDNA(HS2DNA)pH7.25,HS2DNA,HS2DNA524nm,493nm,HS2DNA,ScatchardHS2DNA,HS2DNAZhou[74]2,(AO),DNA(CT2DNA),CT2DNA939©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[75]Scatchard:r/c=K(n2r):r;c;n;Kr/crDNADNADNA,,,[11]3.2.3[76],,DNA,DNA[77,78],XDNA[79282]DNA,DNA3.3DNA[83,84],DNADNA[85,86]Fiel[87]2DNA,,DNA3.4DNA[88,89]DNA,DNADNA,DNA;DNA,DNA;DNA,DNA,,DNA,,,DNA4,DNA,,DNA,,DNA,DNA,;DNA,,DNA;DNA,,,:[1],.DNA[J].,2005,32(5):3112314.[2]WeiX,HaoQL,ZhouQ,etal.InteractionbetweenpromethazinehydrochlorideandDNAanditsapplicationinelectrochemicaldetectionofDNAhybridization[J].Electrochim.Acta,2008,53(24):733827343.[3],,.DNA[J].,2005,16(4):1012104.[4]WatsonJD,CrickF.Molecularstructureofnucleicacids:astructurefordeoxynucleicacids[J].Nature,1953,171:7372738.[5]PengH,ZhangLJ,SoellerC,etal.Conductingpolymers049©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[J].Biomaterials,2009,30(11):213222148.[6]WuJK,HuangCH,ChengGF,etal.Electrochemicallyactive2inactiveswitchingmolecularbeaconfordirectdetectionofDNAinhomogenoussolution[J].Electrochem.Commun.,2009,11(1):1772180.[7]LiF,ChenW,ZhangSS.DevelopmentofDNAelectrochemicalbiosensorbasedoncovalentimmobilizationofprobeDNAbydirectcouplingofsol2gelandself2assemblytechnologies[J].Biosens.Bioelectron.,2008,24(4):7812786.[8]HassenWM,ChaixC,AbdelghaniA.AnimpedimetricDNAsensorbasedonfunctionalizedmagneticnanoparticlesforHIVandHBVdetection[J].Sensor.Actuat.B:Chem.,2008,134(2):7552760.[9],,,.()Fe()SchiffDNA[J].,1998,56(2):1782183.[10],.DNA[J].,2003,66(5):3172322.[11]KumarCV,AsumcionEH.DNAbindingstudiesandsiteselectivefluorescencesensitizationofananthylprobe[J].J.Am.Chem.Soc.,1993,115(19):854728553.[12]LiF,ChenW,TangCF,etal.RecentdevelopmentofinteractionoftransitionmetalcomplexeswithDNAbasedonbiosensoranditsapplications[J].Talanta,2008,77(1):128.[13],.Ni/GC[J].,2000,35(2):1282130.[14].[J].,1998,10(1):45254.[15],,,.[J].,2006,34(12):180121806.[16]UtsunoK,MaedaY,TsuboiM.Howandhowmuchcanhoechst33258causeunwindinginaDNAduplex[J].Chem.Pharm.Bull.,1999,47(10):136321368.[17],,,.DNA2[J].,2001,18(12):9942997.[18],,,.DNA[J].,2007,26(1):59261.[19]GirousiST,GherghiIC,KaravaMK.DNA2modifiedcarbonpasteelectrodeappliedtothestudyofinteractionbetweenrifampicin(RIF)andDNAinsolutionandattheelectrodesurface[J].J.Pharm.Biomed.Anal.,2004,36(4):8512858.[20]NahidS,SoheilaK,AzadehF.Fluorescencespectroscopy,UV2visspectrophotometric,andvoltametricstudiesofthecompetitiveinteractionoftris(bathophenanthrolin)nickel(II)complexandneutralredwithDNA[J].J.Biotechnol.,2008,136:S62.[21]SahooBK,GhoshKS,BeraR,etal.Studiesontheinteractionofdiacetylcurcuminwithcalfthymus2DNA[J].Chem.Phys.,2008,351(123):1632169.[22]TemerkYM,IbrahimMS,KotbM.Voltammetricandspectroscopicstudiesonbindingofantitumormorin,morin2Cucomplexandmorin22cyclodextrinwithDNA[J].Spectrochim.ActaA,2009,71(5):183021836.[23]ShiXL,ChenF,YuJP,etal.Studyofinteractionbetweensmad7andDNAbysingle2moleculeforcespectroscopy[J].Biochem.Bioph.Res.Co.,2008,377(4):128421287.[24]LingX,ZhongWY,HuangQ,etal.SpectroscopicstudiesontheinteractionofpazufloxacinwithcalfthymusDNA[J].J.Photochem.Photobio.B,2008,93(3):1722176.[25]YangZS,ZhangDP,LongHY,etal.ElectrochemicalbehaviorofgallicacidinteractionwithDNAanddetectionofdamagetoDNA[J].J.Electroanal.Chem.,2008,624(122):91296.[26]WangRY,JiMN,WangRQ,etal.Stopped2flowkineticfluorimetricstudiesoftheinteractionofRu(II)complexwithDNAanditsanalyticalapplication[J].Spectrochim.ActaA,2008,71(3):104221048.[27]CorduneanuO,Chiorcea2PaquimAM,GarnettM,etal.Lipoicacid2palladiumcomplexinteractionwithDNA,voltammetricandAFMcharacterization[J].Tal