毛细管电色谱分析碱性药物

CECPHPH7.8PH2.3PHCECCEC3.2/CECLCCECEC//CECCECCECECu-LC4-TRIS2-N--MESSigmaPoole,UKFluka(Poole,UK)AldrichPoole,UK,AZAZ4-pKa1FisherLoughborough,UKRomil(Cambridge,UK)Milli-QPlusWatersSA,StQuentin,France0.2umAerodisc,GelmanScienceLtd,Northampton,UK1CECCECHichromReading,UK,3um33cm25cm100umHichromCEC3umHypersilC18Hypersil33cm25cm100umHypersilC18Hypersil15KV815◦C810ug/ml100ug/ml1:1v/v.15KV214nm50mMEOFHP3dCEHewlettPackard,Waldbronm,GermanyCECu-LCu-LCCECPH2PHEOFPHEOFEOFCECHypersilC18EOFCECPH3PH7.820/SCXPH7.823PH2.34PH7.8PHPH7.82.3EOFPH7.82.3PHt04150mMPO4,PH2.3150mMTEA-PO4,PH2.5/25cm50mMPO4PH2.3/25cm150mMTEA-PO4PH2.517702061930519730AZ4596415365744657629121611133133842CECCEu-LCB-P-T-D-A-AZN-10mMPO4PH2.330mMTEA-PO4PH2.510mMTEOA-PO4PH2.510mMTeOA-TFAcPH2.5CECu-LCCECECCECu-LCCECECCECu-LCCECECCECu-LCCEBABBBTBBBBBBBNBNNNTDBDTNNNNDADDTDPNPTPDDDDNDNADPATANAAAPAATTTBADADADTTATT&PBAPPTNPNPNPPTPPP350MmTEOA-PO4PH2.5702010uecm2*V-1*s-13.17×10-42.56×10-4AZ2.73×10-42.87×10-43.09×10-42.95×10-4PH2.3PH2.5TEA150mMPH2.310.33mm/s0.46mm/sPH2.35CECPH2.54-EOFEOFEOF5PH2.5EOFEOFPH2.5PH2.550mM150mM2CEC60%6670%EOFEOF25KV0.45mm/s5KV0.09mm/s20KV77(TFA)TEOAPH875%80%TFA989CECECCECECEC10CE2000-26000/25cmCEC200-85000/25cmPH2.5-0.28mm/sCEt0TFACECECt0102CECCEu-LCu-LCCECCECCECCEu-LC2TFATEOACECCECPH2.5CEC