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191Vol119No1120042JournalofYunnanAgriculturalUniversityFeb.2004X,,,(,650106):,,:;;;:TQ45;S572:A:1004-390X(2004)01-0110-06AdvanceandApplicationofBiologicalAgentinTobaccoXIAZhen2yuan,ZHUMing2liang,YANGShu2jun,LIYun2hua(KeyLaboratoryofBiotechnology,YunnanAcademyofTobaccoScience,Kunming650106,China)Abstract:Applicationofbiologicalagentisimportantensuringmeasurement.Theconceptofbiologicala2gent,surveyofstudiesandapplicationofbiologicalagentintobaccowerereviewedinthisarticle,existmat2tersandsuggestionwerealsodiscussed.Keywords:tobacco;biologicalagent;study;application,,68,200[1],,,,,,R(Resistance,Resurgence,Residue),2001[2],,,,;;;2090,10%20%21(),,X:2003-05-15:(2001NG08);(01A06);(110200101010):(1971-),,,1,,,,;,,,,;;()[35],,,,,,,,,4:,,,,50008000;,,,;,,,;,,,,,,,2211,,,,4:Royal300(,;Royal350,;Bioact,;DiTer,(Myrotheciumverrucaria),[3],,3111%,12%1[4],,34%,[5]2,93104%98192%.[6]9018%.,011%,5317%.[7]2090[814]1111,:;,,6316%212,,,(),NPV,NPV,GV,CPV,Bt315%,,,,[15][16]3,1%,,1992,[17],[18],BT70%[19](Avmerctins)(Streptomycesavermitilis)16,1981,1994AVMB1[20],,,7334hm2,1500200090%.213,Secharomycescerevisiae,S.ludwigii,Gandidaspp.,Kloeckeraapicu2lata,[21]50,,,,,,300[22][23],80%NF9TC3,()[24]1932,Weindling(Trichodermalignorum),,,,18TV-1,,,,,21119214,PGPR,VA[25],15d414%3010%.[25]18,,TR13[26],(Trichodermalongibrachiatum),9716%,67%,Piperbetle310%8414%8615%,(Clerodendroninerme)(Lantanacamara)Nicotiananudicaulis310%55%6217%.1998,[27,28]2753,187,60%14,(Pseudomonasspp.)(Enterobacterspp.)(Agrobacteriumspp.)(Bacillusspp.)(Bacilluslicheniformis)RB42.80%,215,,B-9605,B-9605;100%,;5710%8418%,[29]23,10HSU[30][31],[32],,AQB-46AQB-33,,230d5412%6417%.[33](Bacillusspp.),830d.,,,,80%.3,20d,215kg/hm2,719,112hm2,6316%,,2hm21110%200090%TV-119993313hm2,60153%,2000320hm2,54164%.199820013111,:,31107hm2.1000,912kg/hm2,,80%,3B-9605,5710%8418%,0S,0S,,,,,EB-82,[19]OS-,4,,,,,,:,,,;,,,,,,,,,,,,,,,WTO,,,,,,,,,;,,,,,,,[][1].().2001.2[2],.[J].,2001,(12):3-5.[3],,,.[J].,1998,16(2):231-233.[4],,.[J].,1998,27(2):196-199.[5],,.[J].,1991,21(4):304-306.[6],.[J].,1994,10(1):7-10.[7].[D].,1997.[8],,,.[J].,1999,(3):16-19.[9],,,.[J].,2002,24(3):238-240.[10],,,.[J].,2002,(1):30-33.41119[11],,,.[J].,2002,29(2):4-8.[12],,,.[J].(),2002,22(5):369-372.[13],,,.[J].,2001,23(3):247-248.[14],,,.[J].,2001,(3):79-82.[15].3.5%[J].,1999,(3):20-22.[16].[J].,2001,7(4):33-35.[17].[J].,1998,(6):45-46.[18].[J].,2000,6(3):32-36.[19],.BT[J].,1997,(3):38-39.[20],.[J].,2000,10:43-44.[21].[J].,2000,(3):47-48.[22].[J].,1999,27(1):71-72.[23],,,.[J].,2000,(4):31-37.[24],,,.[J].,2001,(1):10-12.[25].[J].,1994,9(2):95-100.[26],,.[J].(),2001,(3):222-226.[27],,,.[J].,2001,(2):93-95,98.[28],,.[J].,2001,(3):244-246.[29],.[J].,1997,23(3):256-260.[30]HSUST,CHANGML.Effectofsoilamendmentsonsur2vivalofPseudomonassolanacearum[J].PlantProt.Bull,1989,31:21-23.[31],,.[J].,1994,10(3):106-109.[32],,,.[J].,1999,(4):16-19.[33],.[J].,1999,(2):94-95.[34]..1999(http:),2003-8-23.5111,: