pCDH-CMV-MCS-EF1-GreenPuro-CD513B-1

LentiStarterKit™AnEasySamplerforOptimalLentiviralPackaging,Concentration&TransductionSystemBiosciences’LentiStarterKitprovidesallyouneedtoexperiencesuccessfulpackagingofyourlentiviralconstructsintoVSV-Gpseudoviralparticles.Lentivectorconstructsareco-transfectedwiththepPACK™PackagingMixinto293TNProducerCellsforrobustvirusproduction.PseudoviralparticlesareharvestedfromthecellculturemediumandconcentratedusingSBI’sone-stepvirusconcentrationsolution,PEG-itTargetcellsarethentransducedwithhightiterviralparticlesusingLentiBreneforstableintegrationandexpression.™.LentiviralTechnologyHighlightsesamplekittono-ni-llatneinevnoC•maketransduciblelentiviralparticlesnoitarenegdr3htiwelbitapmoC•lentiviralconstructsdr3ynafoyreviledtneicffieylhgiH•generationlentivectorconstructrotcevitnelsserpxeylbatS•constructsinawiderangeofmammaliancells)lm/UFI9^01(delbaneretithgiH•—suitableforin-vivoapplicationssalsoitcudorphcae—ytilibixelF•availableindividually™TransductionMixpPACK™PackagingMixYourLentivectorConstructpVSV-GPackagingPlasmid(s)PseudoviralParticles5xPEG-it™SolutionCellCultureMediumContainingViralParticlesastrocytes(GFP)PhasecontrastGFPHumanembryonickidneycells(RFP)HumanEmbryonicH9Cellsdirectlytocells132ComponentAmount1pPACKH1-PlamidPackagingMix40μl2PEG-it5ml3LentiBrene(500x)24μlLentiStarterKit(cat#LV050A-1)HumanPrimaryNeurons(GFP)AnimalModelsMouseCarotidartery(GFP)PrimaryCellsDownloadLentiStarterKitProtocol(pdf)LentivectorExpressionFormats©2009SystemBiosciences,Inc.Allrightsreserved.SystemBiosciencesandtheSystemBioscienceslogoaretrademarksofSystemBiosciences,Inc.SystemBiosciences,Inc.1616NorthShorelineBlvd.MountainView,CA94043TollFree:888.266.5066Fax:[phone]Email:[e-mail]•Strongandubiquitousexpressionofthegeneofinterest•Singleordoubleexpressioncassettewithchoiceofreportergene•TargetgeneexpressedfromCMV,EF1,orMSCVpromoter•ChoosefromFIV-orHIV-basedvectorsMCSAmpR3’∆LTRWPRESV40poly-AcPPTSV40ORIpUCORIRSVCMVEF1α5’LTRMCS5’XbaINheIEcoRIBstBISwaIBamHINotI3’MarkerpCDH-CMV-MCS-EF1LentivectorSeriesMarkerOptionsMarkerCatalog#PuroCD510B-1GFPCD511B-1RFPCD512A-1NeoCD514B-1HygroCD515B-1GFP+PuroCD513B-1MCS5’XbaINheIEcoRIBstBISwaIBamHINotI3’RFPGFP-or-pCDH-EF1-MCS-IRESCat.#CD530A-1(RFP)Cat.#CD531A-1(GFP)IRESseriesIRESMCS5’XbaINheIEcoRIBstBISwaIBamHINotI3’PuroGFP-or-pCDH-EF1-MCS-T2ACat.#CD520A-1(Puro)Cat.#CD521A-1(GFP)T2AseriesT2AcopGFPcPPTAmpRWPRERSVpUCORISV40poly-A5’LTRSV40ORI3’∆LTRH1CMVPuroT2ApeptidepGreenPuro™shRNAVectorcat.#SI505A-1~7.8kbPuropSIH1-H1/pSIF-H1shRNAVectors~7.2kbcopGFPH1PermanentRNAKnockdownwithshRNAlentivectors•StableexpressionoftheshRNAtargetingyourgene•Choiceofsingleordoublemarkers•Dissectsignalingpathways•DiscovernewdrugtargetsshRNAExpressionOptionsSystemBiosciencesoffersawide-rangeofcustomservicestosupportyourresearch,allowingyoutospendlesstimemakingtools,andmoretimemakingdiscoveries.Tolearnmore,visitourwebsiteat