运动对非酒精性脂肪肝大鼠血清酶活性和肝脏抗氧化能力的影响

29520099JournalofGuangzhouSportUniversityV0129No5Sept2009(226001)SD34CHFHE3ASTALTLDHSODMDAcHFASTLDHALTMDASODHFHEASTA1LDHMDASODG8005A1007323X(2009)05010503EffectsofAerobicExerciseandtheSupplementofSoyPolypeptideontheFreeRadicalMetabolizeandUltrastructureofHighfatdietRatsWANGWei-jun(ScienceCollegeofPENantongUniversityNantong226007JiangsuChina)AbstractObjectiveToexploretheeffectsofaerobicexerciseandthesupplementofsoypolypeptideonthefreeradicalmetab-olizeanduhrastructureofhishfatdietratsMethods60SDratsweredividedrandomlyintofivegroupsCgroupHFgroupHEgroupHSgroupHSEgroupTheaetivityofASTALTandLDHinserumandthecontentsofMDAandSODintheliverweredetectedattheendofexperimentwhilethemorphologicalchangesofthelivertissueswereobservedundertransmissionelectronmicroscopeResultsComparedwithCgroupTheactivityofASTandLDHinserumofHFgroupincreasedsig-nificantlytheactivityofASTinserumvendupwardsbutnotobviouslyTheconcentrationofMDAincreaseddramaticallyTheSODactivityintheliverdecreasedsignificantlyComparedwithHFgrouptheactivityofASTALTandLDHinserumofHEgroupHSgroupandHESgroupdecreasedsignificantlyTheconcentrationofMDAdecreaseddramaticallyTheSODactivityintheliverincreasedsignificantlyConclusionsTheresearchresultsindicatedbothaerobicexerciseandthesupplementofSPIcouldpromotetheclearanceoffreeradicalsandimprovetheultrastructuredamagesofhighfhtdietratsThroughthedoublefactorvarianceanalysisexerciseandthesupplementofSPIdisplayedsignificantinteractioninincreasingtheSODactivityandloweringt}IeconcentrationofASTandLDHinserumandMDAinhishfatdietsratsliverKeywordsaerobicexercisefattyliverdiseasesoypolypeptidefreeradical20090620(1974)(IK040192)10529(NAFLD)}12JlNAFLDNAFLD'4JNAFLD111SD(tl,)34514016093C(10)HF(14)HE(10)3525-I-3C551560min71HE1128014205313HE60min615minlO20min160min10100cm70cmX60cm50cm321oC1412h50mgKgBW2lOml2h40C4000rpmtl,lOmin20ASTALTLDH1cm40391060863ml103500rpmtl,10min20CSODMDA15151ASTALTLDH7150152SODMDASODMDAuV245016SPSSl304-(XS)tP005P0012211ASTALTLDHO05O01HFP005}+PO011CHFALT(P005)HFASTLDHASTALTLDH22SODMDA2SODMDAc+P005}+PO01HFP005P0012CSOD(P001)MDA(P001)HESOD(P001)MDA29(P005)SODMDA(P001)3NAFLD6JASTALTLDHSODMDA31ASTALTLDHLDHHJALTAST1ALTLDH1LDHALTNAFLDASTLDH(P001)ALT(P005)HEASTALTLDH32SODMDANAFLDNAFLD3JHFSODMDASODMDA(P001)o111214JHFMDA441ASTALTLDHMDASOD42[1]PaulAnguloNonalcoholicFattyLiverDiseaseNEnslJMed200234612211231[2][J]20029(5)273275[3]JanardanKReddyMSambasivaRaoLipidMetabolismandLiverInflammation11FattyliverdiseaseandfattyacidoxidationAmJPhysiolGastrointestLiverPhysiol2006290852858[4]YoosooChangSeunghoRyuEunjuSungeta1HigherCon-centrationsofAlanineAminotransferasewithintheReferenceIntervalPredictNonalcoholicFattyLiverDiseaseClinicalChemistry200753686692[5]ChoudhuryJArunJInsulinresistanceandthepathogencsisofnonacholicfattyliverdiseaseClinLiverDis20048(3)575[6]LoonAAdamsPaulAnguloKeithDLindorNonalcoholicfattyliverdiseaseCMAJ200529(7)172[7][M]200069[8]BurgertTSTaksaliSEJamesDziuraeta1AlanineAminotransferaseLevelsandFattyLiverinChildhoodObesityAsso-ciationswithInsulinResistanceAdiponectinandVisceralFatJClinEndoefinolMetab20069142874294[9]DamI,arsenSFranzmannMAndersenIBeta1LongtermprognosisoffattyliverriskofchronicliverdiseaseanddeathGut200453750755[10]GoreMFiebigRHollanderJEndurancetrainingaltersan-tioxidantenzymegeneexpressioninratskeletalmuscleCanJPhysiolPharmacol199876(12)1139145(114)1072933l8(LBW)(PO01)6150min34150min456[1][J]1996(2)[2][M]20008[3][J]1989[4][M]19916[5]M]1985[6][M]19895+-++++++-+-+-++-----+--+--+-+++-+-+-+-+-+-+-+-+-+-+-+-+-+--+-++---6--(107)[11][J]200625(3)282285[12][J]200018(4)7678[13]114[J]20022l(6)609[14]DamianMBYoungISMcEnenyJeta1Regulationoffreeradicaloutflowfromanisolatedmusclebedinexercisinghu-mallsAmJPhysiotHeartCircPhysiol200428716891699