Vitrobot应用指南 (1)

VitrobotVitrificationofsuspensionsAPPLICATIONINSTRUCTIONSApril5,2002Semi-automaticfastfreezingofthinfilmsofsuspensionsundercontrolledtemperatureandhumidity.Switchingthesystemon.1Makesuretheliquidnitrogen/ethanevesselisnotontheliftbeforeyouswitchthesystemon.2Openvalveonthewallforairpressuretooperatetheblottingmechanism.3SwitchPCon.Waittillthestartupprocesshasfinished.4DoubleclickontheVRicontostartthevitrobotsoftware.5Thesystemwillinitializeandputthetweezerdown6Press“placenewgrid”(plungerwillmovetomountingpositionforthetweezer)7Mountagridinbetweenthetweezertipsandslidetweezerwithlittlespringpointedatyouoverthev-shapedendoftheplunger.SeeFig2forpositionoftheslideronthetweezer.Putsliderinthefirstslitonthetweezer.8Adjusttothetemperatureyouneed(rangefrom6°Cto50°C)9Setyourdesiredvaluesfortheparameters,orloadyourstartsettings.10ToggleOFFthe“autoraiseethanelift”11Adjusthumidityto100%,buttoggleONthe“humidityoffduringblotting”12StartfillingtheouterringandethanevesselwithliquidNitrogentocooldown.ThiswilltakesometimebeforetheliquidNitrogensettles.Thenremovetheliquidfromtheinnerspaceandstartfillingitwithethanegas.13Onceethaneliquifiesraisetheflowandfillthespacejustabout2mmbelowtheedge.Figure1Figure2VitrificationofsuspensionsAPPLICATIONINSTRUCTIONS14Takeup3to5µlsampleinapipet;starttheapplicationprocessandapplythesampleoncethetweezerisdowninthechamber,throughtheopeningintheleftsideofthevitrobot.15Hitthefootpedalagaintostarttheblottingandautomaticplunging.16Removethetweezerfromtheplungerandtransferthegridtothegrid-boxsittingintheliquidNitrogenouterringoftheethanevessel.17Takeupanewgridandstartagainatstep7.Switchingthesystemoff.1Afterlastblottingprocedureputthetweezerbackandbringitinthechamber.2Pressthexboxupperrightcorner(endsVRprogram)3switchcomputeroff(donotleaveitonovernight!!)4closeairpressurevalveonthewall