搜索
1,2,3(1.,537000;2.,210037;3.,530004)[][],,[](F=82.99,Pr0.0001),13.63mg/g,20.09%;(F=33.48,Pr0.0001),6.86mg/g,25.82%;(F=11.63,Pr0.0001),4.81g,19.45%;(F=14.46,Pr0.0001),20.25mg,34.14%8[];;;S664.3A0517-6611(2007)25-07878-03ResearchonVariationofMedicineQualityIndicesofGinkgobilobaL.CrossingGenerationMOZhao-zhanetal(YulinNormalCollege,Yulin,Guangxi537000)Abstract[Objective]ThestudyaimedtochooseGinkgobilobaL.familylineswithhighflavoneandterpenelactonescontentsaccurately.[Method]With1-year-oldhybridfilialgenerationseedlingsofginkgobeingcontrolledpollinationastestmaterials,theflavoneandlactonescontentsofhybridfilialgenerationweredeterminedbyHPLCandthevariationofmedicinequalityindicesofginkgohybridfilialgenerationwasstudiedthroughanalysisofvari-ance.[Result]Thetotalflavonecontentinleaveshadextremelysignificantdifferenceamongfamilylines(F=82.99,Pr0.0001),withtheaverageof13.63mg/gandvariationcoefficientof20.09%.Thetotallactonescontentinleaveshadextremelysignificantdifferenceamongfamilylines(F=33.48,Pr0.0001),withtheaverageof6.86mg/gandvariationcoefficientof25.82%.Thebiomassperplanthadextremelysignificantdifferencea-mongfamilylines(F=11.63,Pr0.0001),withtheaverageof4.81gandvariationcoefficientof19.45%.Thevalideconomicoutputofleavesperplanthadextremelysignificantdifferenceamongfamilylines(F=14.46,Pr0.0001),theaveragewas20.25mgandvariationcoefficientwas34.14%.8goodleaffamilylineswerescreenedbyclusteringanalysispreliminary.[Conclusion]Theecologicalfactorsanddifferentseed-resources,vari-eties,timesofleafpicking,treeages,positionsofleafpickingandprocessingmethodinfluencedflavoneandlactonescontentsinginkgoleaves.KeywordsGinkgobilobaL.;Hybridprogeny;Flavone;Lactones(Ginkgobiloba),,20(),,[1],[2-3],,,,11.14,23,1234,5;5,,ABCDE,20,(CK),,,100/,31,93,15,(BG2004314)(1974-),,,,,2007-04-285048h,,,3,=2.51(++);=(A+B+C+),315,,80,,=(+)1.21.2.1Waters;Waters510;996;AlltechELSn2000;Millipore-QA(GA)B(GB)C(GC)(BB);100200();;1.2.2:AlltimaC18(4.6mm250mm,5m);:-0.3%(4357);1.0ml/min;360nm;35;20l;:AlltimaC18(4.6mm250mm,5m);:(201275);0.9ml/min;ELSD100;2.8L/min;20l;1.2.3:0.8g,(,JournalofAnhuiAgri.Sci.2007,35(25):7878-788020001IXH1),,100ml,25%=41,25ml,,,1h,,,,,,,0.45m,:0.9g,(20001H1),,,(3060)100ml,701h,,,,80ml3h,,2ml,5ml(100200,5g,10mm),20ml,,10ml,,,0.45m,1.3,EXCELSAS22.1,(F=82.99,Pr0.0001)13.630mg/g,20.09%,,51E1C3CK4A2E,38.1%35.4%31.2%29.7%26.7%54E2A2C3E3D,25.7%26.7%36.5%38.2%38.8%(1)12.2,(F=33.48,Pr0.0001)6.863mg/g,25.82%,,52B2E4E1E4CK,46.7%37.3%30.1%21.7%18.4%51D1A4C2A2C,20.2%26.0%38.2%40.1%57.3%(2)2.3,(F=11.63,Pr0.0001),244.814g,19.45%,,53C3A4A4B1A,43.6%26.0%22.2%13.8%13.2%51CK4E2E4CK2D,19.4%20.3%22.3%22.8%24.0%(3)204.937g,44.196g,117.6%,232.4,(F=14.46,Pr0.0001)20.252mg,34.14%,,54A3B3A1C1E,58.64%53.98%46.98%40.22%34.02%51D2C4CK2A4E,32.15%32.50%37.25%37.84%52.18%(4)42.52.5.11,13.63mg/g,6.86mg/g,20.49mg/g[2-5],,,97873525,,,51E2E1C3CK4B;53D3E1A2A2C4A3B3A1C1E;1D2C4CK2A4E,,20.09%25.82%28.41%1mg/gmg/gmg/ggg1A10.875.0815.951.2219.431B12.355.6618.011.2923.201C18.457.6726.121.0928.401D11.915.4817.390.7913.741E18.828.3527.171.0027.141CK12.866.2919.150.8115.612A10.004.1114.110.8912.592B11.8210.0721.890.6714.702C8.662.9311.591.1713.672D12.757.6320.390.7815.962E17.289.4226.700.6617.642CK15.956.8322.780.6615.033A16.356.9323.281.2829.773B14.527.9922.501.3831.183C12.936.2219.151.4026.823D8.347.8916.231.1318.263E8.427.7916.211.0717.403CK17.887.3325.210.8922.474A17.686.0123.691.3532.134B16.707.9924.691.0225.214C15.184.2419.411.0119.654D14.855.7520.611.1523.644E10.128.9319.050.519.684CK12.458.1320.570.6212.7113.636.8620.490.9920.252.5.2,2,,24,0.90,5,1:254132314235133CK;2:454CK2CK242233443534121CK144311;3:232153(1),,[6-8][9-11][12-13][14-16](2),,,,,,,,,,,,,,(3),2,,24,31,8,24,;2,14;3,2,,24[1].[M].:,1999.[2],,,.[J].,2000,36(4):26-31.[3],,,.[J].,2002,29(10):928-935.[4],,,.[J].,2003,23(3):195-198.[5],,,.[J].,2002,33(11):5201-5203.[6],,,.[J].,1992,23(3):122-124.[7],,,.[J].,1997,22(2):106.[8].[J].,1997,24(3):215-219.[9],,.[J].,1997,28(4):211-212.[10].[J].,1997,17(3):283-285.[11],,,.[J].,2000,31(2):88.[12],.[J].,2000,22(5):368.[13],.[J].,1997,28(3):342-346.[14].[J].,1998,7(3):1-7.[15],,.[J].,2000,22(5):46-48.[16].[D].:,2003.08872007