间作药材与接种内生真菌对连作花生土壤微生物区系及产量的影响

20103082105—2111ActaEcologicaSinicahttp//*[e-mail]112*1221.2100462.210008B3Archishypogaea。B3、SOD。Atractylodeslancea/Euphorbiapekinensis9%－22%B324%。/B3B330%4%。B3P59%B3PB328%B3PEB313%B3PB3。B3B3。TheeffectsofintercroppingwithmedicinalplantsandadditionofendophyticfungionsoilmicrofloraandpeanutyieldDAIChuanchao1XIEHui1WANGXingxiang2*LIPeidong1LIYilin2ZHANGTaolin21CollegeofLifeScienceNanjingNormalUniversityNanjing210046China2KeyLaboratoryofSoilEnvironmentandPollutionRemediationInstituteofSoilScienceChineseAcademyofSciencesNanjing210008ChinaAbstractThedeteriorationofsoilmicrofloraandthedeclineofenzymeactivityhavebeenregardedastwokeyfactorsofthereductioninpeanutyieldinlong-termcontinualpeanutcroppingsystem.Tounderstandsoilbiologicalfunctionsinpeanutcroppingsystemasurfacelayer0－20cmindepthofredsoilwassampledfroma15-year-oldcontinuouspeanutcroppinguplandattheEcologicalExperimentalStationofRedSoilChineseAcademyofScienceslocatedinYingtanJiangxiprovinceN28°13′E116°55′withanaltitudeof45mASLandpotexperimentswereconductedtoinvestigatetheeffectsofintercroppingwithmedicinalplantsandaddingendophyticfungiB3Phomopsissp.onsoilmicroflorasaccharaseureaseactivitiesandpeanutsuperoxidedismutaseSODactivityandpeanutyield.TheresultsshowedthatintercroppingwithmedicinalplantsandaddingendophyticfungiB3treatmentssignificantlyreducedsoilmouldcolonyformingunitsCFUbutenhancedbacteriumCFUandsaccharaseactivity.ThebacteriaincreaseandmouldreductionfollowedtheorderofAtractylodeslanceaintercroppingpeanutwithaddingB3treatmentPAB3peanutmonocroppingwithaddingB3treatmentPB3EuphorbiapekinensisintercroppingpeanutwithaddingB3treatmentPEB3A.lanceaintercroppingpeanuttreatmentPAE.pekinensisintercroppingpeanuttreatmentPEcontrolpeanutmonocroppingP.ComparedtothecontrolPsoilsaccharaseactivitywassignificantlyincreasedatfloweringpeggingstageinthePAandPEtreatments.ItsincreasewasgreaterafteraddingB3.ThehighestwasobservedinthePAB3http//、PAPEP.ComparedtothecontrolPthePAandPEtreatmentsrosethepeanutyieldby9%－22%whileanincreaseof24%wasinPB3treatment.AddingB3increasedtheyieldby30%and4%inPAB3andPEB3treatmentsthanPAandPEtreatmentsrespectively.CombiningtheintercroppingandB3addingincreasedtheyieldby59%greaterinthePAB3thaninthecontrolPbutonly13%increasewasfoundinthePEB3treatment.TheseresultssuggestedthatsomesynergismsmighttakeplacebetweentheintercroppingA.lanceaandadditionofB3forimprovingpeanutyieldwhilethepresenceofE.pekinensismightinhibitB3activitytosomedegreeanditsmechanismneedmoreinvestigation.KeyWordspeanutmedicinalplantintercropcontinuousmonocroppingobstaclesendophyticfungi、1。2-3。4-8。59。、310-12。9、Atractylodeslancea、Ophiopogonplatyphyllum、Euphorbiapekinensis、PinelliaternateDiosorenzingiberebsis556。+/31%18%39%35%13。B314-17。B3。11.128°13′116°55′10—20cm15a。13.1g·kg－1pH4.961∶2.5H2ON0.76g·kg－12.48mg·kg－1POlsen-P14.65mg·kg－1K258.40mg·kg－1CEC10.34cmolkg－1。Archishypogaea5。A.lanceaE.pekinensis。B3Phomopsissp.14Bischofiapolycarpa。PDAPDA25℃7dPD250mL50mLPD25℃150r/min6d2。1.2610。P、B3、601230http//、B3、B3PAB3PE、B3、B3PEB3。20064321。。23cm、23cm8.0kg1g、2gCa3PO422gK2SO4。20073。4233。523B310g。823。113060℃。、、20d10dSOD80d7、、、、。1.3、、。118。35-19-21。mg·g－137℃24hNH3-Nmg·g－137℃24h。SODSOD22。1.4SPSS13.0One-WayANOVADuncanP=0.05。22.1B3PPA、PEB3PB31。PA、PEB3PAB3、PEB3。B3。B3PAB3B3B3PEB3B3PB3。B3。B3。。1B3Table1EffectsofmedicinalplantsandendophyticfungusB3onsoilmicrobialcommunityTreatmentBacteria/×106CFU·g－1DMⅠⅡActinomycete/×106CFU·g－1DMⅠⅡMould/×104CFU·g－1DMⅠⅡYeast/×104CFU·g－1DMⅠⅡP7.61±0.60a5.70±0.24a3.72±0.20a0.77±0.03ab8.63±0.99c5.68±0.28c0.57±0.63a0.32±0.12aPA8.10±0.25a7.71±0.45b4.11±0.12a0.98±0.02b3.73±0.11a4.48±0.12b0.95±0.59a0.51±0.04aPE9.04±0.08b6.83±0.24b3.98±0.48a0.72±0.11a4.92±0.25b5.44±0.28c0.86±0.95a0.32±0.12aPB315.4±0.28d11.0±0.74c4.61±0.54ab0.85±0.04ab4.00±0.49a4.72±0.24b0.77±0.52a0.47±0.16aPAB316.7±0.36e13.5±0.52d5.62±0.49b0.77±0.05ab3.64±0.13a3.63±0.21a1.87±0.43b0.95±0.21bPEB314.3±0.28c12.1±0.36c4.72±0.16ab0.72±0.07a3.80±0.05a4.27±0.24b0.58±0.71a0.55±0.12aPpeanutAA.lanceaEE.PekinensisB3forEndophytestrainⅠFloweringpeggingstageⅡPodfillingstage0.0570128http//。2.2B3PPAPEB3PB324%33%B3PAB3PEB3B3PA、PE21%14%27%13%。B3、。B3PAB3B3B3PEB3B3PB3。B3PAB3、PEB3B3。2B3Table2EffectsofmedicinalplantsandendophyticfungusB3onsoilenzymeactivitiesTreatmentSaccharase/mg·g－1d－1ⅠⅡUrease/mg·g－1d－1ⅠⅡP3.23±0.07a5.78±0.79a0.13±0.01b0.09±0.01aPA3.51±0.13b6.76±0.65b0.11±0.01a0.10±0.01abPE3.36±0.04a6.53±0.87b0.13±0.01b0.09±0.01aPB34.00±0.17d7.66±0.33c0.15±0.01c0.10±0.01bcPAB34.25±0.12e8.56±0.43d0.18±0.01e0.12