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,,,;,,,,,,,,1.63.,1991,12(4):160.2..,1986,(6):30.3..,1999,18(4):48.4..,2002,17(1):33-35.5.20,2000,(3):17.6.168.,1982,(11):36.7.33.,1991,14(2):56.8,.81.,1996,15(8):352.9..,1986,(9):45.10..,1998,20(4):47.11,,..,1998,13(75):33-34.12,..,2002,23(3):275-276.13.1.,2002,12(4):256.14,,.50.,1999,17(11):31.15..,1994,(4):27.16,..,1995,(5):36-37.17..,1994,25(6):41.18..,2002,37(12):526.19..,1983,4:31.(:)(IRI),,0335.0mIU/L;:0mIU/LIRI,3808,10.1mIU/LIRI,30350,152.0mIU/LIRI,357399,335.0mIU/LIRI,684567:,95%105%:N=60CV5%(IRI),,,,(IRI);;;;R446.6A1680-077X(2007)11-0995-03AbstractObjectiveToexploreareliablemethodforthedeterminationofinsulin.MethodsGatherfastingagglutinatingblood,centrifugalizethemrespectivelytogettheserum,whichweredirectlysetoninstrumenttodetermine.ResultsLinearrang:0335.0mIU/L,senitivity:themeanquantumof0mIU/LIRI,10.1mIU/LIRI,152.0mIU/LIRIand335.0mIU/LIRIwere3808,30350,357399and684567.Specificity:whenthecontrolserumwasdetermined,themeasurevlaluewaswithinthestandarddeviation(SD),95%-105%.Precision:N=60CV5%.ConclusionChemiluminescenceisareliable,stablemeasurementmethodfordeterminationofinsulinwithhighsensitivity,precisionandspecificity.Keywordsinsulin;quantum;preproinsulin;proinsulin;chemiluminescence:515100,599ChineseJournalofMedicine2007711(IRI),IRIIRI,(1200)11024(9000),C-[1]IRI,IRIA21,B30;C-31IRIIRI,IRI[2,3]11.1IRI,;,,,IRI1.2:BAERACS-180()1.3,,:25l+50l375min,250l372.5min,,300l,,,1.4,0.0mIU/L138.0mIU/L,,22.1,60,IRI220.8mIU/L,CV1.8%;,10,211.2mIU/L,CV2.8%2.20mIU/LIRI,3808,10.1mIU/LIRI,30350,152.0mIU/LIRI,357399,335.0mIU/LIRI,684567IRI0335.0mIU/L2.3IRI25mIU/L1mg/L500g/LC-1mg/L1mg/L1mg/L,:100.8%,95.1%,96.6%,100.2%,101.6%2.4,,IRI:IRI0335.0mIU/LIRI335.0mIU/L,,2.5(Hb5g/L)(0.2g/L),2.6100,50,50,1955,37IRI:20.517.5mIU/L(P0.05)3IRI,,,,3,,,,,,,,,,,:,,,,2,,,(IRI)1,.,2.:,2001,256.699ChineseJournalofMedicine20077112ChevenneD,TrivinF,PorquetD.Insulinassaysandreferencevalues.DiabetesMetab,1999,25(6):459-476.3McAuleyKA,WilliamsSM,MannJI,etal.Diagnosinginsulinresistanceinthegeneralpopulation.DiabetesCare,2001,24(3):460-464.(:),,R-33A1680-077X(2007)11-0997-03(metabolicsyndrome,MS),(),MSDM5,CVD3,2,1.5[1],,11.1Sprague-Dawley(SD)100,,4970g,,,:SCXK()200500151.2,[2,3],:56%,10%,10%,10%,12%,2%,GB1445,,81211F3,11.3OlypusAu2700(),,:OSR6121YP600(),(),TDL-5():250355,22.1SD100,2:20(),80(),,,,2025,50%70%,,81(g/100g)35ð.019O.6014ð.079.8420ð.013f.224ð.013O.445Ù.02O.81Ù.009.561Ù.009.56-10“-10“-10“-12“17Ù.0214O.337Ã.9122O.6848Ù.2041O.492Ã.6419.62(kCal/g)3Ã.3249.27(%)20Ù.5013O.4121Ù.4447O.7558Ù.0638O.83799ChineseJournalofMedicine2007711

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