酿酒葡萄品种蛇龙珠亲缘关系分析

chenyaofeng
4 ℃
2018-01-04

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@163.com36RAPD14DNASSR41.41-2.243.163.183.32RAPDSSR1.70CABERNET1892209011214SSRCABERNET22.1RAPD141.Cot2.(Carignan)3.(Syrah)4.(PinotN)5.PinotB6.RieslingItalien7.CheninB8.RieslingB9.Cabernetsauvignon10.~15ngDNA941min45s9430s3645s722min457210minSSRVVMD20ul2.0ul10buffer,1.0mmol/LmgCl2,200umol/LdNTPs,20pmol,0.5UTaqDNA10ngDNA942min9230s5230s722min40727minVVS10ul1.0ul10buffer,1.5mmol/LmgCl2,200umol/LdNTPs,25pmol,0.625UTaqDNA25ngDNA955min941min5050s50~721min721min26727minRAPD1.2%SSR6%2.4DDSTATISTACAUPGMA33.114RAPD3.1.1RAPD36361414114137DNA9.89871.5%3-107X10213S462S347(1)88.9%3.1.2RAPD2143.32-7.483.327.48(3)6.01412343(%)S238TGGTGGCGTT10660.0S462TCGGCACGCA9888.9S347CCTCTCGACA9888.9S450TCAGAGCGCC9444.4S35TTCCGAACCC10987.0S119CTGACCAGCC9777.8S262ACCCCGCCAA12975.0S94GGATGAGACC9777.8S445CCCAGTCACT9444.4S446CCACGGGAAG10880.0X102TACCGTGGCG131076.9X101TTTGCCGCCC11654.5X105CCACACGTAG11981.8X115CGCGTTCCTG6350.0Total1379871.51.RAPDS3472.SSRVMD61PCRmarker1-14.1.12PCRmarker01-061.1Fig.1.TheampliedresultsofRAPDprimersS347Fig.2.TheampliedresultsofSSRprimerpairsVMD6Fig.1Fromlefttoright:PCRmarker1~14.Experimentalsamplenumberslistedin1.1MaterialsFig.2:Fromlefttoright:PCRmarker01~06.Experimentalsamplenumberslistedin1.1Materials5.206.006.242.533.544.555.566.5714548673132101211913.14UPGMA1.1Fig.3.TheUPGMAclusteranalysis14winegrapesSee1.1Materialsforvarietyname3.2SSR3.2.1SSR12SSR62.RAPDTable3.ThesequenceandresultsofselectedRAPDprimersprimersequence5-3NumberofamplifiedbandsNumberofpolymorphicbandsRateofPolymorphicbands(%)VMD5CTAGAGCTACGCCAATCCAATATACCAAAAATCATATTCCTAAA44100VMD6ATCTCTAACCCTAAAACCATCTGTGCTAAGACGAAGAAGA66100VMD7AGAGTTGCGGAGAACAGGATCGAACCTTCACACGCTTGAT44100VMD8TAACAAACAAGAAGAGGAATAGCACATCCACAACATAATG4250VVS1ACAATTGGAAACCGCGTGGAGCTTCTCAATGATATCTAAAACCATG2150VVS2CAGCCCGTAAATGTATCCATCAAATTCAAAATTCTAATTCAACTGG44100VVS3TGCCCTATCAATTAGTTCACCTATCGACTTTGATATATTGATGATT2150VVS4CCATCAGTGATAAAACCTAATGCCCCCACCTTGCCCTTAGATGTTA2150VVS5ATTGATTTATCAAACACCTTCTACATTAGAAAGATGGAAGGAATGGTGAT11100Total292482.8(4)3.36/3123LinkageDistance