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276200811JournalofFoodScienceandBiotechnologyVol.27No.6Nov.2008:167321689(2008)0620115206:2007211226.:(19682),,,.3:(19622),,,,,.Email:yxu@jiangnan.edu.cn1,2,1,2,1,2,31,2(1.,214122;2.,214122):(HS2SPME)(GC2MS)395,73;465250,233TIC23:;;;;;:O657.63:ADeterminationofVolatileCompoundsinFruitWinebyHeadspaceSolidPhaseMicroextraction(HS2SPME)FollowedbyGasChromatography2MassSpectrometry(GC2MS)ZHANGYing2lu1,2,FANWen2lai1,2,JIANGWen2guang1,2,XUYan31,2(1.KeyLaboratoryofIndustrialBiotechnology,MinistryofEducation,JiangnanUniversity,Wuxi21422,China;2.SchoolofBiotechnology,JiangnanUniversity,Wuxi214122,China)Abstract:Arapidandsimplemethodwasdevelopedfordeterminationofaromacomponentsoffruitwinebyheadspacesolidphasemicroextraction(HS2SPME)coupledtocapillarygaschromatographymassspectrometry(GC2MS).Atotalof95volatilesweredetectedinthreefruitwines,and73volatilecompoundswereidentifiedbyGC2MS.Ofthese,thesewere46,52,and50volatilecompoundsidentifiedinplumwine,licheewine,andmedlarwine,respectively.Furthermore,23compoundswereexistedinallfruitwinesstudiedinthismanuscript,ofwhich,ethyloctanoate,octanoicacid,and2phenylethylalcoholandsoonwerethemainvolatilecompounds.Keywords:headspacesolidphasemicroextraction(HS2SPME);fruitwine;gaschromatography2massspectrometry(GC2MS);plumwine;licheewine;medlarwine,,,12%()[1],[2],,1mg/L1g/L,1ng/L,,(HS2SPME):,[3],,,[4][2,4,5]HS2SPME(GC2MS)3,,11.11.1.1:;:;:1.1.2NaCl:,();:Sigma2Aldrich()1.1.3Agilent6890N5975(GC2MS);:DB2Wax(30m0125mm0125m);GerstelMPS:1121121110mL,20mL,3gNaCl,11212SPMECAR/DVB/PDMS,5min,5030min11213GC2MS:250,:50,2min,3/min230,10min;HeNIST05aTIC2211SPMESPME,PAPDMSPDMS/CARCAR/DVB/PDMS4,CAR/DVB/PDMS3,PA,,HS2SPME;,,,30405060,5020,30,40,60min,30min[4],30%NaClSPME:CAR/DVB/PDM,5030min,30%NaCl2121,GC2MS,NIST05a1(a)61127(b)(c)1(a),(b)(c)2Fig.1GC2MStotalionicchromatogramofvolatilecompoundsin(a)plumwine,(b)licheewine,(c)medlarwine1HS2SPMEGC2MSTab.1ThevolatilecompoundsinthreefruitwinesweredetectedbyHS2SPMEandGC2MSRId/%acids1449aceticacida,b,c0.210.400.831842hexanoicacida,b,c0.630.240.641936heptanoicacida,b,c0.04NDND2051octanoicacida,b,c0.330.964.412133sorbicacida,b4.012.310.842264decanoicacida,b,c0.140.232.322446benzenecarboxylicacida,b,c0.030.020.012488dodecanoicacida,b,c0.040.03ND2908hexadecanoicacida,b,c0.11NDNDalcohols900ethylalcohola,b42.3244.4440.7210291212propanola,b,c0.150.420.7010762221222methyl212propanola,b0.120.160.397115:2RId/%11361212butanola,b,c0.07ND0.1011792221222methyl212butanola,cNDND0.4311933221232methyl212butanola,b0.627.017.4212431212pentanola,c0.02ND0.0413481212hexanola,b,c0.060.100.451354232212(z)232hexen212ola,bNDND0.031377232212(e)232hexen212ola,bNDND0.0314832221222ethyl212hexanola,bND0.080.0915092222nonanola,cNDND0.1015471212octanola,b,cND0.070.1215642,322,32butanediola,b,cND0.070.6016531212nonanola,b,cNDND0.041860benzylalcohola,b,c0.530.030.03189822phenylethylalcohola,b,c0.222.731.76esters892ethylacetatea,b,c1.431.804.671022ethylbutyratea,b,cND0.220.22103522ethyl22methylbutyratea,b,c0.30NDND105132ethyl32methylbutyratea,b,c0.220.160.18111323232methylbutylacetatea,b,cND1.360.701263ethylhexanoatea,b,c0.301.152.931292hexylacetatea,bNDND0.071315ethylheptanoatea,b,c0.020.02ND132922ethyl22hexenoatea,cNDND0.02133122ethyl22hydroxypropanoatea,bND0.591.011411ethyloctanoatea,b,c1.567.100.0514962,42ethyl2,42hexadienoatea,b0.510.15ND1603butyrolactonea,b,cND0.02ND1629ethyldecanoatea,b,c0.581.141.031649ethylbenzoatea,b,c4.150.190.081667diethylsuccinatea,b,c0.361.121.84168592ethyl92decenoatebND0.26ND17852ethyl2phenylacetatea,b,c0.160.160.05175122methyl22hydroxybenzoatea,b0.170.030.06180522phenylethylacetatea,b,cND0.260.141878ethylbenzenepropanoatea,b,cNDND0.312133ethylcinnamatea,b,c0.090.010.062250ethylhexadecanoatea,b,c0.090.06NDaldehydesandketones1073hexanala,b,c0.11NDND1446furfurala,b,c0.310.490.841503benzaldehydea,b,c13.59NDND15872222undecanonea,bNDND0.14benzenes1245styrenea,bNDND0.10terpenesandnorisoprenoids811272RId/%1169d2d2limonenea,b,c0.030.10ND13352trans2roseoxidea,bND0.10ND15402linaloola,b,cND0.710.08168122terpinenola,b,cND0.67ND1721cadinenea,bND0.06ND1714naphthalenea,b,c0.180.08ND178622citronellola,bND1.33ND180222damascenonea,b,c0.03ND0.0318231212methylnaphthalenea,b0.03NDND183422Iononea,b0.06NDND192422iononea,b,c0.05ND0.042034nerolidol2a,bNDND0.04phenols2002phenola,b,c0.040.020.012036isoengenola,b0.23NDND21684242ethylphenola,bNDND0.4421804222methoxy242vinylphenola,b,cNDND0.0324062,422,42bis(1,12dimethylethyl)phenolb0.030.260.27sulfides900dimethylsulfidea,bND0.22NDfurans15942222furancarboxylicacidethylesterbND0.050.0619472,522,52furandicarboxaldehydea,bND0.04ND250052()22252(hydroxymethyl)222furancarboxaldehydeb0.920.08ND:d:(22);a:RI;b:94%;c:ND:1,HS2SPMEGC2MS395,73,17239114531,146,5250212111,39,3(9105%),,,21212,1,,,31222212322121226,322122,322127%(),816212133,23,310,323,,[6-7]212149115:[8-9]113,36,74,52222121554311,,13159%,[10],,,[11],12,,3HS2SPME,,,,3,73,233,12222123221212232222,42TIC,322122,3,(References):[1].[M].:,1991.[2]WangL,XuY,ZhaoG,etal.Rapidanalysisofflavorvolatilesinappl