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27320085JournalofFoodScienceandBiotechnologyVol.27No.3May.2008:167321689(2008)0320120205:2007204209.:(19832),,,.3:(19622),,,,,,Email:bioch@163.com1,32,1,2(1.,214122;2.,214122):(Aspergillusniger)E2562,:240g/L(),120U/g,508h,(DP)118119:2;;;:Q53;Q556.2:AStudyonHydrolyticConditionsofKonjakGlucomannanby2MannanaseXUChun2mei1,WUMin2chen32,LIJian2fang1,DENG2chao2(1.SchoolofFoodScienceandTechnology,JiangnanUniversity,Wuxi214122,China;2.SchoolofMedicine,JiangnanUniversity,Wuxi214122,China)Abstract:Thehydrolyticconditionsofkonjakgumforproducingglucomannanoligosaccharideswerestudiedwithhighactivity2mannanasefromAspergillusnigerE256.Basedonsinglefactortest,theoptimumconditionswereobtainedbyorthogonaltest.Theoptimumhydrolyticconditionswereasfollows:konjakgumsolution240g/L(withdeionizedwater),hydrolytictemperature50,2mannanasedosage120U/gkonjakgum,andhydrolytictime8h.Withtheseconditions,therangeofaveragepolymerizationdegree(DP)was1.81.9inhydrolysates.Keywords:2mannanase;konjakgum;glucomannanoligosaccharides;orthogonaltest(Konjakglucomannan,KGM)D2D211161117,21,421,3[1]2KGM,210(konjakglucomannanoli2gosaccharides,KGMOS)[2]KGMOS,,,,[326]KGMOS2,[7];,40g/L,[8]1030g/L,,[9],E2562(),(,1520min2),1030g/L240g/L,,11112Aspergillusni2gerE256,,3284h,242000U,2,12000U/mLpH70315,70pH215810,Al3+Ca2+,Ag+112:;:Sigma;(PK04,40,KGM70%416%011%015%12%SO2013%310%):;113113112[10]:214mL510g/L(pH4182)011mL,(50012)15min;DNS,1mol()12(U)113121)KGM()01100g,0101mol/L2(pH312)40mL,4h50mL,4000r/min20min,KGM4mL25mL,015mL6mol/LH2SO4,,115h,NaOH10mL015mLDNS,(1):=(g)(g)100%(1)2)01250g,250mL,70mL2h100mL,4000r/min20min,215mLDNS,(2):=(g)(g)100%(2)3)KGM019(),(3):=(g)-(g)(g)019100%(3)113132,,DNS()(4):=(g)-(g)(g)100%(4)11314(DP)1,,118119[7](DP)=(g)(g)(5)2211,1[11],=0146,CV=0157;=010939,CV=212,1213:80119%,4130%,KGM68130%1Tab.1Determinationofthetotalsugarandfreereducingsugar/%/%KGM/%180.304.1468.54280.764.2568.86379.544.4167.62480.614.3568.63580.614.4168.58679.704.2267.93779.854.3167.99880.154.2868.2880.194.3068.3021221211pH80U506h,pH(80g/L)(2)2,pH515615(47%);(pH610)pH310510(50%),pH3152pH315[12],,[8,13]2pHTab.2EffectofthepHofkonjakgumsolutiononhydrolyticratepH/(g/L)/%3.038.430.2754.550.413.540.010.4257.010.664.038.580.5454.770.854.537.880.8553.690.545.036.390.6551.360.8035.810.3250.460.505.533.580.5846.980.916.031.350.4943.510.776.527.030.5936.770.912121280g/L506h,(DP)(3),,,,[8],DP118119,7080U3DPTab.3EffectofthedosageofenzymeonreducingsugarandDPofhydrolysates/(U/g)/(g/L)DP603114521047033176119080351421181903610411781003715211711103811211682121380g/L50406080U/g,DP(1)1,DP,DP,DP,,;,DP118119,406080U/g,1012h79h56h1Fig.1EffectofhydrolytictimeontheDPofkonjakhydrolysates21214DP(4),4060,DP,,DP118119,221274090U,507080U,6060U4DPTab.4EffectoftemperatureonthereducingsugarandDPofkonjakhydrolysates//(U/g)/(g/L)DP408033.241.939035.641.8010035.841.79507035.051.838035.641.809036.451.76606035.441.817036.871.748038.881.6521215506h,DP(2)2,,DP,200g/L,DP119,100U50,46810h(3)3,DP118119,8h2Fig.2EffectofkonjaksolutionconcentrationontheDPofhydrolysates213213113,L9(34)(5)5,R=0117,R=0106DP118119,,:120U/g,240g/L508h3Fig.3EffectofhydrolytictimeontheDPofkonjakhydrolysates5Tab.5DataandResultsofL9(34)orthogonaltest/(U/g)/(g/L)/hDP11(80)1(220)3(10)1.8822(100)11(6)1.9733(120)12(8)1.77412(240)21.9252231.9063211.85713(260)12.1182321.9093331.77K15.915.625.93K25.775.675.59K35.395.785.55k11.971.871.98k21.921.891.86k31.801.931.85R0.170.060.1321312,3(6)6,3DP118119,6Tab.6Verificationoftheoptimumhydrolyticconditions/(U/g)/(g/L)/hDP112024081.85212024081.87312024081.863213:3(Aspergillusniger)E2562,,:240g/L(),50120U,8h(References):[1]WilliamsPA,FosterTJ,MartinDR,etal.Amoleculardescriptionofthegelationmechanismofkonjacmannan[J].Biomacromolecules,2000,1(3):440-450.[2],,,.2[J].,2005,14(6):115-118.XUMu2dan,KELei,ZENGQiao,etal.Investigatedproductionofmannan2oligosaccharidesusingkonjakpowderby2mannanmannohydrolasefromAspergillusniger[J].ActaAgriculturaeBoreali2occidentalisSinica,2005,14(6):115-118.(inChinese)[3],,,.[J].,2002,23(6):41-44.WUYong2jun,WANGJia2fu,CAIJin2teng,etal.Thegrowthpromotingeffectsonbifidobacteriamfromextractsandproductsofkonjacglucomannanoligosaccharides[J].FoodScience,2002,23(6):41-44.(inChinese)[4],,,.[J].,2001,32(2):142-144.YANGYan2yan,LIXiao2ming,LIShun2yi,etal.Effectofkonjacoligosaccharideonbloodsugarandantioxidantabilityofmice[J].ChineseTraditionalandHerbalDrugs,2001,32(2):142-144.(inChinese)[5],,.[J].,2002,23(4):181-182.CHENLi,YANGYan2yan,YANDa2zhong.Theeffectsofkonjacoligosaccharidesondecreasingbloodlipid[J].ChineseJournalofBiochemicalPharmaceutics,2002,23(4):181-182.(inChinese)[6].ICR[J].,1998(4):3-4.LIXiao2ning.RegulatingeffectsofsyrupusKGMOSonthefemaleICRmiceimmunologicfunction[J].JiangsuPreventiveMedicine,1998(4):3-4.(inChinese)[7],,,.2[J].,2007,33(1):21-24.LIJian2fang,WUMin2chen,CHENGKe,etal.Studyonthepreparationofoligo2glucomannanusingkonjakgumby2mannanase[J].FoodandFermentationIndustries,2007,33(1):21-24.(inChinese)[8],,,.2[J].,1996,22(1):14-17.YANGWen2bo,TONGShu2min,SHIWei,etal.Astudyonenzy