制备型高效液相色谱技术的研究进展

,,,(,510600)[],,,[];;TheDevelopmentofPreparativeHighPerformanceLiquidChromatographyWeiLi,ZhangKun,FangYanxiong,ZhaoShuqin(GuangdongUniversityofTechnology,Guangzhou510600,China)Abstract:Preparativehighperformanceliquidchromatographyisakindofquickandeffectiveseparativemeans.Inthispaper,thebasictheoriesandinstallationswerereviewed,andthepretreatmentofsamplesandapplicationin2stanceswereintroduced,theexistingproblemsofthepresentpreparativeHPLCwereanalyzedandthedevelopingtrendwasalsoforecasted.Keywords:preparative;highperformance;liquidchromatography[]2005-09-14[](1979-),,,,:,,,,[1]HPLC,,,,,863[2],,,,1,:1.1940Wilson[3],,;2.1941MartinSynge[4],,,;3.Amundson[5],,,;4.,;5.Funk,,,,,,[6],,10%[7],,[8]2HPLC()(),,HPLC,5200511©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(),10mL,,,,,,2.3,,,(DACTM)[9]DACHPLC,100500mm,,,,,,[10]2.4,,,,,2.5,,,,,,[11],,2.6HPLC,,HPLC33.1HPLC,,,,,,[12],,,,,,3.2,HPLC3,HPLCHPLCHPLC,11HPLC3HPLC/cm/cm/m0.52155010301050mg5207040600.11g105050100406020g/dHPLC,,3.3HPLCHPLC[13],,,HPLC,,,HPLCHPLC,,,,,;,,HPLC,[14]HPLC,HPLC,,,,3.4,,6200511©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[15],HPLC,,[16]4,25%,98%,60%[17],HPLC,83.3%,3.1%,98.61%,[18][19]YZS-3B,;[20];[21]HPLC,99%[22]Deltaprep3000[23]HPLC,6,99%[24]HPLC,4,[25]HPLCAUGPAGP3[26]5,[27],,,,,HPLC-AAS()HPLC-LC()HPLC-HPLC-HPLC-HPLC-NMR()HPLC-IR(),HPLC(),HPLC,(),,HPLC,[1],,.[J].,2001,17(1):88-96.[2]CretierG,neffatiJ,RoccaJL.preparativeLCandpreparativeSFC:twocomplementarytechniquesinthefractionationofanimpurityfronamayorcompo2nent[J].Chromatogscren,1994,32:449-454.[3]Wilson.J.N.,J.Amer[J].Soc,1940.62:1583.[4]Martin.A.J.P.,SyngeR.L.M.,Biochem.J[J].1941,35:1358.[5]AmundsonN.R.,LapidusL.,J.Phys[J].Chem.,1952,56:984.[6],,.[J].,1999,12(3):44-46.[7].[J].,1990,8(5):308.[8]R.A.Barford,B.J.Sliwinski,H.L.Rothbart.InteractionofproteinswithBondephaseIonExchanges[J].J.Chromatogr,1980,185:393.[9]JoonKoh,B.ScottBroyles,HongGuan-sajionz,etal.Consolidationandcol2umnperformanceofseveralpackingmaterialsliguidchromatographyinady2namicaxialcompressioncolumn[J].J.Chromatogr.A,813,1998,223.[10],,.[J].,2001,1:28-30.[11],,.[J].,1997,11(1):83-87.[12].-[J].,1994,18-20.[13],.[J].,2004,93.[14],,.[J].,1997,27(4):92-96.[15],.[M].,:,1998,199-200.[16]k.,A.,M..[M].2000.14.[17],,.[J].,2004,148.[18],,.[J].,2004,408.[19].YZS-3[J].,1986,4(2):98.[20],.[J].,1990,25(12):940.[21],,.[J].,1981,16(6):460.[22],,,[J].,2004,35:285-287.[23],,.Deltaprep3000[J],1998,9(2):129.[24],,.[J].,2004,1(5):359-361.[25],,,.[J].,1999,27(8):924-926.[26],,,.[J].,2004,22(2):184-186.[27],,.[J].,1997,11(5):12-17.7200511©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.