SCI修改稿回答审稿人意见范文模板

DearEditorsandReviewers:Thankyouforyourletterandforthereviewers’commentsconcerningourmanuscriptentitled“RealtimeMonitoringofXylitolFermentationbyMicro-RamanSpectroscopy”(LANL-2014-0001).Thosecommentsareallvaluableandveryhelpfulforrevisingandimprovingourpaper,aswellastheimportantguidingsignificancetoourresearches.Wehavestudiedcommentscarefullyandhavemadecorrectionwhichwehopemeetwithapproval.Revisedportionaremarkedinredinthepaper.Themaincorrectionsinthepaperandtherespondstothereviewer’scommentsareasflowing:Respondstothereviewer’scomments:1.Responsetocomment(Reviewer1):(Theproposedmethodwasestablishedwiththelackoftheprocessoftheoptimization.Thecurvewasadoptedtoillustratethechangesofabsorptionpeakintheprocessoffermentation,buttheabsorptionpeakcouldn’tbeconfirmedtheidenticaltothereferencepeakofxylitol.Itneedsmoredatatoprovethereliabilityofthemethod)Response:ConsideringtheReviewer’ssuggestion,theexperimentofhighperformanceliquidchromatography(HPLC)isaddedtoverifythereliabilityoftheRamandata.Ramanpaekisakindofinelasticscatteringpaek（notabsorptionpeak）,Ramanspectrumofthestandardxylitolandxylosesolutionswasuesdtoselectethemostappropriatecharacteristicbandsofxylitolandxyloseforourexperimentbasedontheircomparativestrengthwithoutoverlapwithotherbands.2.Responsetocomment(Reviewer1):(Theliteraturedata(table1)wasusedinthispaper,butdifferentshiftsandstrengthwouldbeobtainedusingdifferentRamanspectrometers,sodatashallbecarriedoutaccordingtotheexperimentalresultsinthesupportofliterature.)Response:differentexperimentalconditionshasagreatinfluenceonthepeakintensity.butlittleeffectonthepeakramanshift,ThisisthebasisoftheRamanqualitative.3.Responsetocomment(Reviewer1):(Thereferencesshowsdifferentstyles.)Response:Theformatofthereferencehasbeencorrected.4.Responsetocomment(Reviewer2):(Themediumisacomplexmixtureandincludesyeastextract.YeastextractwillcontainmanyofthecharacteristicRamanpeaksthattheauthorsascribetotheyeastcells.)Response:everysampleofyeastcellspallethadbeenwashedtwice（suspensionandcentrifugation）toensureimpurityelimination,inaddition,yeastextractisobtainedafterplasmolysisandcompletehydrolaseautolyzed,removethecellwallandinsolubleproductsaccordingtotheproductionprocessofyeastextract.sotheprocessforpurifingtheyeastcellspalletisreasonable.5.Responsetocomment(Reviewer2):(Thespectralresolutionisstatedtobe1.2cm-1;however,thespectraaresmoothed....Nodetailsofthesmoothingalgorithmusedareprovided.Thisaffectsthespectralresolution..)Response:weaddedtheprocessingmethodsofRamanspectrainsectionofDataanalysis.6.Responsetocomment(Reviewer2):(InFigure3,thepeakat866cm-1.....intoxyluloseviaxylitolasintermediate.(page7lines21to32))Response:Wetrytocorrectthedeficienciesinthispassage.WethinkthisisnecessarytotheoreticallyverifytheRamanconsistentwiththefactthatweknow.Alsoralatetothediscussionontheby-products.8.Responsetocomment(Reviewer3):(Itissaidthatinorganicsaltisanimportantfactorforthefermentationprocess(Page3,line36),butthereisnodiscussorexplorationaboutthis)Response:inorganicsaltisanimportantfactorforthefermentationprocess，buttheeffectofinorganicsaltisnotthefocusofthearticle，theconcentrationofinorganicsaltisadjustedtothemostsuitableconditionsforthisstrainaccordingtothereference.9.Responsetocomment(Reviewer3):(Figure4wasusedtoillustratethechangesofbiologicalmacromolecules,butalltheRamanpeaksherewerenotclear)Response:FigureinthefirstdraftofthepaperhasaproblemintheexportsizebyOrigin8.5，andwehavemodifiedthesizeofresubmittedfiguretoensurebetterviewofpeak.10.Responsetocomment(Reviewer3):(Theassignmentofbandsintable1alldependedonlectures.)Response:TheRamancharacteristicbandsofbiologicalmacromoleculararecommon-senseconclusioninthisfield,infact,almostallarticlesinvolvedbiologicalRamanbandsofBiologicalmacromoleculehavedirectquotedtheseconclusions.threeexampleswerelistasbelow:SchusterKC,UrlaubEandGapesJR.2000.Single-cellanalysisofbacteriabyRamanmicroscopy:spectralinformationonthechemicalcompositionofcellsandontheheterogeneityinaculture.Journalofmicrobiologicalmethods.42(1):29-38.BaşarG,KınS.2008.MonitoringofspectroscopicchangesofasingletrappedfissionyeastcellbyusingaRamantweezersset-up.OpticsCommunications.281(19):4998-5003.XieC,LiY.2003.Confocalmicro-Ramanspectroscopyofsinglebiologicalcellsusingopticaltrappingandshiftedexcitationdifferencetechniques.JournalofAppliedPhysics.93(5):2982-2986.Wetriedourbesttoimprovethemanuscriptandmadesomechangesinthemanuscript.Thesechangeswillnotinfluencethecontentandframeworkofthepaper.Andherewedidnotlistthechangesbutmarkedinrevisedpaper.WeappreciateforEditors/Reviewers’warmworkearnestly,andhopethatthecorrectionwillmeetwithapproval.Onceagain,thankyouverymuchforyourcommentsandsuggestions.YoursSincerelyZhenHuang