组织工程用水凝胶材料

Vol.17200412JournalofFunetionalPolymersl).J””,,(,,20043:,.,,:;;;;:063:A:189357(24)468,9,.,,,3,,.,l1.1,,,,,,,,,__[6],;,,.8,,,ABA,BYamamot,:20040722:(No.29825109No.20174006No.20221402No.2()374015)l.};973(No.G199905430603)863(No.2004AA21517):(1971),,,,:En1;lilzllw_ley0Ucom:,,,,:Email:,ddlnfud.d,.,690,,1.2,,,,,2[1s,,,,3,n,1.3,(GAG),laiurcichitosanFig.1Chemiealstruetureofhyaluronieaeid,ehitosan,andsodiumalginate[lv,3,31,3,5[z0.11.4,,,,,,,,2;CDPGYIGSR,1.5N,40%ND,1,;,,,,,,,Matthew,,;1.6,1,[sz,33CaZM+BaZ+Sr+,,,,,,,,,Mooney[ssRGD(ArgGlyAsp),CohenCa,+,,24h90%,,4d,,,,22.12(PHEMA)37,2:CH3}CHZC!C))HZCHZOHPHEMACH3CHZC}C0}NHCH3CHCH3PNIPAAmHOCHZCHZOHPEOHeH:CHZHeHZmeHZCHZHCHZCHPE(~PP)PEOFig.2ChemiealstruetureofPHEMA,PNIPAAm,PEO,PEO-PPPEO,andPVAPHEMA,PHEMA,[39PHEMA,Meyvis[#0PHEMA,ParkPHEMAN(PNIPAAm),2,PNIPAAm(LCST),LCST,,PNIPAAmLCST32,,PNIPAAmPNIPAAm,PNIPAAm,PNIPAAm,,43N,,6PNIPAAm,,HuhPNIPAAm,,2.2(PEO),(FDA),,,2,PEO,,PEO,PEO;PEO,PEO(DCC),PEOAla--PrGly-Leu,PEO,PEO,PEO,,[slPEG,MannRGDPEG,,RGD,PEO,PEOPPO()(PEPPPEO),PluroniPoloxamers,PEO,,,,;,,,,PEPPPEO,,,PEGPPGPEO,,5,,,PEOPLA,[34,5,;,[602.3(PVA),,2PvA[sl[62,PVA,PVA,PVAPVA,2,,RGDS(Arg-Gly--AsPSer)PVA,KobayashiRGDSPVA2.4,[66a-,,,(/)[6s(/)[60(/):,pH2.5,,,CapPenDNA,,,,Tirrell,,Gly()Ala(),pHGly(Val()Pro()Gl),,,3[8l,sz][8s[s,,,,,;,,,,,,,,,,,;,,,;pH,pH,,[ss,LCSTLCST,,LCST,,LCST,,(LCST),[8s88],,,,[3Langer[ssPEO,PEO,,:;1.5mm,1005,PEO80%;PEO,,3minPEO,7,,(GAG)HubbeU[s0,5%;RGD,RGD1.0102mol/mZ,50%,1.ox10mol/m,,RDG,4,.694,,,,,,;,,,,,,:LangerR,VaeantiJP.TissueengineeringJ.Scienee,1993,260:920926.LangerR.Ideasarelikehildren[J.TeehnologyReview,1999,102:7477.,,.[.,,1999,19.,.M.:,2003.4969.Raultl,Freiv,HucA,etal.Evaluationofdifferentchemiealmethodsforroslinkingollagengel,filmsandsponges[J.JMaterSciMaterMed,1996,7:215221.ChevallayB,AbdulMalakN,HerbageD.Mousefibroblastsinlontermeulturewithineollagenthree-dirnensionalseaffolds:In-ueneeofcrosslinkingthdiphylphosphory]azideonmatxreorganizatson,growth,andbiosthetieandproteolytieaetivities[J.JBiomedMaterRes,2000,49:448459.AugerFA,RouabhiaM,GerrnainL,etal.Tissue--engineeredhumanskinsubstitutesdevelopedfromeollagepopulatedhydratedgels:eliniealandfundamentalapplieatlonsJ.MedBiolEnginComput,1998,36:801812.SeliktarD,BlaekRA,NeremRM,etal.Dynamiemeehaniealconditioningofollage,gelbloodvesseleonstruetsindueesremode-linginvitro[J].AnnBiomedEng,2000,25:351362.KaufmannPM,HeimrathS,MooneyDJ,etal.Highlyporouspolymermatrieesasathree--dimensionaleulturesystemforhepato-eytesJ.CellTransplantation,997,6:463465.VoytiHarbinSL,BrightmanAO,LamarCH,etal.smallintestinalsubmueosa:Atissuderivedextraeellularmatrixthatpromotestissue-speeificgrowthanddifferentiationofcellsinvitroJ,TissueEng,1998,4:157174.YamamotoM,TabataY,IkadaY.GrowthfaetorreleasefromgelatinhydrogelfortissueineeringJ.JBioaetCompatPolvm,1999,14:474489.PerkaC,SpitzerRS,SchultzO,etal.Matrimixedeulture:Newmethodologyforehondroeyteeultureandpreparationofearti-lagetransplantsJ].JBiomedMaterRe:,2000,49:305311.YeQ,HubbellJA,Tu6naM,etal.FibngelasathreedimensionalmatrixineardiovaseulartissueengineeriJ.EurJCar-dioThoraeSu,2000,17:587591.IkariY,FujikawaK,SchwartzsM,et1.^lpha()proteinaseinhibitor,alpha()antiehymotrypsin,oralpha(2)maeroglobulin15requiredforvaseularsmoothmuseleeellspreadinginthredimensionalfibringel[J].JBiolChem,2000,275:1279912805.MeinhartJ,FusseneggerM,HoblingW.Stabilizationoffibrin-ehondroevteconstruetsforcartilagreeonstruetionJ.AnnplastSurg,1999,42:673678,,,.nC.,,2002,178180.VereruysseKP,MarecakDM,PrestehGD,etal.Synthesisandinvitrodegradationofnewpolyvalenthrazideerosslinkedhydrogel:ofhluroniaeid[J.BioeonjugateChem,1997,8;686694.PouyaniT,HarbisonGS,PrestehGD.Novelhrogelsofhyaluronieaeid:synthesis,surfaeemorphology,andsolidstateNMRJ.JAmChemSoe,1994,116:75157522.ChoiYS,HongSR,NamYS,etal.Studiesongelatin-eontainingartifieialskin:11.preparationandeharaeterizationofeross-linkedgelatin-hyaluronate,ponge[J.JBiomedMaterRes,1999,48:631639.RadomskyM,SwainL,PoserJ,etal.LoealadministrationofbasiefibroblastgrohfaetorinahyaluronieaeidgelaeeeleratesfraeturehealingJ.JBOneMineralRes,1996,11:M667M667.GjrottoD,UrbaniS,AbatangdoG,eral.ssue-speeifiegeneexpressionjnchondroeytesgrownthree-dimensionalhyaluronieaeidseaffoldsJ.Biomaterials,2003,24:32653275.YalpaniM.Polysaeeharides:Synthesis,modifieations,andstrueture/propert,esrelations[M.1988,Elsevier:Amsterdam.IlonGP,YuXJ,BellamkondaRV,etal.TheinflueneeofPhysiealstruetureandehargeonneuriteextensionina3DhydrogelJJ,10r.JL.L.L[4][56JR„rIJLl..n”11O1,ILLL.LJ11,j1”.rLlesL,..JdŽ”3Žb1,...rwetrlLL,IJ,.J6.,lLILJ,JQ†0,l,rLL21[2223)695seaffoldJ.JBiomaterSciPolymEd,2995,9:10491069.24putnamAJ,MooneyDJ.TissueengineeriDgusingsyntheticextraeellularmatrieesJ.NatMed,1996,2:824826.[25BOrkenhagenM,elemeneeJF,AebiseherP,etal.Tbree-dimensionalextraeellularmatrixengineedinthenervoussystem[J.JBiomedMaterRes,1998,40:392400.[26Cheite^,ChaputC,wangD,etal.Anovelinjeetableneutrax,olutionsofchitosanformbiodegradablegelsinsitu[J.Bioma-terials,2000,21:21552161.27MiFL,Kuaney,ChaSF,etalThestudyofgelationkinetiesand