生物化学与分子生物学PPT素材可根据需要选择取舍Metabolismof5-fluorouracil(5-FU)5-FUCarac,Efudex5-Fluorouridine5-FUMP5-FdUMPUridinephosphorylase5-FluorodeoxyuridineThymidinephosphorylaseOrotatephosphoribosyltransferaseThymidinekinase5-FUTP5-FdUTPRNADNA5’-nucleotidase5’-nucleotidaseUridinekinaseATPADPATPADPPRPPWesternBlotting1.ResolveproteinsamplesonSDS-PAGE6.IncubatetheblotwithchemiluminescentHRPsubstrateandexposetofilm2.ElectrophoreticallytransferfractionatedproteinsfromgelontoPVDFmembrane3.Blockthemembranewithneutralprotein(BSAormilkcasein)4.Incubatethemembranewithprimaryantibodyspecifictotargetprotein5.IncubatethemembranewithHRP-labeledsecondaryantibodyspecifictoprimaryantibodyCholesterolSterolToolkitPregnenolonProgesteronAldosteronCortisolCorticosteroneAndrostendioneTestosteronEstriol17-Estradiol1,25-DihydroxycholecalciferolCholicacidCovalentmodificationofproteinsPoly(ADPribosyl)Polymerase(PARP)Poly(ADPribosyl)polymerase(PARP)NADNicotinamidePoly(ADPribosyl)polymerase(PARP)Poly(ADPribosyl)chainelongationandbranchingNicotinamideNADNADDNApolymeraseDNApolymeraseDNApolymeraseOligosacharyltransferaseN-glycosylationpathwayinERMann-P-DolicholtransferasesFlippaseDolicholPPDolicholPPDolicholPPDolicholPPGlcNAc-PtransferaseGlcNActransferasePDolicholPPDolicholPPGDP-MannosyltransferasesGlc-P-DolicholtransferasesDolicholPDolichol-pyrophofphataseCytosolER40S60SUTPCTPTMPPyrimidineNucleotideInterconversionsUMPATPADPH2OUDPNucleosidemonophosphatekinaseATPADPNucleosidediphosphatekinaseATPADPCTPsynthaseRibonucleotidereductasedeoxyUDPNucleosidediphosphatephosphohydrolasedeoxyUMPThymidylatesynthaseN5,N10-MethyleneTHFDHFTTPNucleosidemonophosphatekinaseNucleosidediphosphatekinaseMyo-Inositol-3-phosphateIns(3)P1BiosynthesisandmetabolismofphosphoinositidesGlucose6-pgosphateIns(3)P1synthaseIns(3)P1phosphataseMyo-InositolInsPtdInssynthasePtdInsMyo-inositolkinasePtdIns-4-PPtdIns4-kinasePtdIns5-kinasePtdIns-4,5-P2PI3KPtdIns-3,4,5-P3PLCPLCPI3KPtdIns-3,4-P2Ins-1,3,4-P3Ins-1,3,4,5-P4Ins-1,4,5-P3PLCPTENPTENIns-1,4-P3PTENPTENFattyacidsynthase-ketoacylACP-synthaseFattyacidssynthesisAcetylCoAcarboxylaseATPADP+PiAcetylCoAMalonylCoACitrateOxaloacetateATPADP+PiCitratelyaseAcetyltransacylaseMalonyltransacylasePantetheine-ketoacylACP-reductasePantetheine-hydroxyacylACP-dehydratasePantetheineEnoyl-ACPreductasePantetheineNADPHNADPNADPHNADPCholesteroldenovosynthesisIIsoprenylformationAcetoacetyl-CoAThiolaseHMG-CoAsynthaseMevalonate3-Hydroxy-3-methylglutaryl-CoAMevalonatekinasePhosphomevalonatekinaseMevalonate-5-pyrophosphodecarboxylase5-Phosphomevalonate5-Pyrophosphomevalonate3-Isopentenylpyrophosphate3,3-DimethylalylpyrophosphateIsomeraseHMG-CoAreductase2NADPH2NADPATPADPADPATPADPATPCholesterolBiosynthesisofbileacids7-HydroxycholesterolSterol7hydroxylaseCYP7A17-Hydroxy-4-cholesten-3-one3-HydroxystroiddehydrogenaseHSD3B77,12-Dihydroxy-4-cholesten-3-oneSterol-12-hydroxylaseCYP8B15-Cholestan-3,7,12-TriolOxosteroid-5-reductaseAKR1D1Sterol27-hydroxylaseCYP27A13,7,12-Trihydroxy-5-cholestanoicacidCholicacidAKR1D1AKR1C45-Cholestan-3,7-diol12-Deoxycholicacid3-Hydroxy-steroiddehydrogenaseAKR1C4BiosynthesisofRNARNAPolymeraseBiosynthesisofDNADNAPolymeraseβ-oxidationMitochondrialmatrixIntermembranespacePalmitoylcarnitinePalmitoylcarnitinePalmitoylCoAPalmitoylCoATransΔ2enolopalmitoylCoA3-HydroxypalmitoylCoA3-KetopalmitoylCoAMyristoylCoAAcetyl-CoAPalmitoylCoAPalmitatePalmitoylCoAligaseATPAMPCarnitineacyltransferaseICarnitineCarnitineacyltransferaseIIAcylCoAdehydrogenaseEnoylCoAhydratase3-HydroxyacylCoAdehydrogenaseCitricAcidCycleFADFADH2NADNADHCytoplasmH2OMyo-Inositol-3-phosphateIns(3)P1Phospho-myo-inositolToolkit2Myo-Inositol-3,4-diphosphateIns(3,4)P2Myo-Inositol-3,6-diphosphateIns(3,6)P2Myo-Inositol-3,4,6-triphosphateIns(3,4,6)P3Myo-Inositol-1,3,4-triphosphateIns(1,3,4,)P3Myo-Inositol-3,4,5,6-tetraphosphateIns(3,4,5,6)P4Myo-Inositol-1,4,5-triphosphateIns(1,4,5)P3Myo-Inositol-1,3,4,6-tetraphosphateIns(1,3,4,6)P4Myo-Inositol-1,3,4,5-tetraphosphateIns(1,3,4,5)P4Myo-Inositol-1,4,5,6-tetraphosphateIns(1,4,5,6)P4Myo-Inositol-1,2,3,4,6-pentaphosphateIns(1,2,3,4,6)P5Myo-Inositol-1,3,4,5,6-pentaphosphateIns(1,3,4,5,6)P5Myo-Inositol-1,2,3,4,5-pentaphosphateIns(1,2,3,4,5)P5Myo-Inositol-1,2,4,5,6-pentaphosphateIns(1,2,4,5,6)P5Myo-Inositol-1,2,3,4,5,6-hexaphosphateIns(1,2,3,4,5,6)P6(Phyticacid)Myo-Inositol-3-phosphateIns(3)P1Phospho-myo-inositolToolkitMyo-Inositol-3,4-diphosphateIns(3,4)P2Myo-Inositol-3,6-diphosphateIns(3,6)P2Myo-Inositol-3,4,6-triphosphateIns(3,4,6)P3Myo-Inositol-1,3,4-triphosphateIns(1,3,4,)P3Myo-Inositol-3,4,5,6-tetraphosphateIns(3,4,5,6)P4Myo-Inositol-1,4,5-triphosphateIns(1,4,5)P3Myo-Inositol-1,3,4,6-tetraphosphateIns(1,3,4,6)P4Myo-Inositol-1,3,4,5-tetraphosphateIns(1,3,4,5)P4Myo-Inositol-1,4,5,6-tetraphosphateIns(1,4,5,6)P4Myo-Inositol-1,2,3,4,6-pentaphosphateIns(1,2,3,4,6)P5Myo-Inositol-1,3,4,5,6-pentaphosphateIns(1,3,4,5,6)P5Myo-Inositol-1,2,3,4,5-pentaphosphateIns(1,2,3,4,5)P5Myo-Inositol-1,2,4,5,6-pentaphosphateIns(1,2,4,5,6)P5Myo-Inositol-1,2,